Changes in Soil Properties, Content of Cd, and Cd-Resistant Bacterial Community with Biochar After One-Time Addition Under Soybean Continuous Cropping and Crop Rotation Patterns
In recent years, Cadmium (Cd) pollution in soybean farmland is severe. Therefore, this study focused on whether biochar influences soil physiochemical properties, the Cd content in soil and soybean grains, and the abundance and community structure of the <i>czcA</i> gene. Four doses of r...
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2025-04-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/15/5/1084 |
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| author | Qin Yao Gege He Shubo Yan Jie Song Yuetong Zhang Xiaoyu Zhang Yan Sun Yibo Wang Yongjuan Li Yu Tian Changjiang Zhao Yongxia Guo |
| author_facet | Qin Yao Gege He Shubo Yan Jie Song Yuetong Zhang Xiaoyu Zhang Yan Sun Yibo Wang Yongjuan Li Yu Tian Changjiang Zhao Yongxia Guo |
| author_sort | Qin Yao |
| collection | DOAJ |
| description | In recent years, Cadmium (Cd) pollution in soybean farmland is severe. Therefore, this study focused on whether biochar influences soil physiochemical properties, the Cd content in soil and soybean grains, and the abundance and community structure of the <i>czcA</i> gene. Four doses of rice husk biochar (0, 5, 15, and 25 t·ha<sup>−1</sup>) were applied under continuous cropping and crop rotation systems, and soil samples were collected after four years of one-time addition. The results indicated that biochar addition significantly increased soil available nitrogen, phosphorus, and soil organic carbon contents under continuous cropping and rotation. Biochar application significantly reduced the total Cd content of soil samples and soybean grains. Additionally, biochar application reduced <i>czcA</i> gene abundance in soybean soils by 14.26–37.88% and 35.96–48.71%, respectively. Correlation analysis revealed that Cd content and the abundance of the <i>czcA</i> gene significantly correlated with soil nutrients and pH. High-throughput sequencing revealed that the relative abundances of several Cd-resistant microorganisms were decreased by biochar addition. In addition, adding biochar significantly affected the Cd-resistant microbial community structure and diversity by influencing soil properties and Cd content. Therefore, this study has important practical significance for improving the soil environment and ensuring the quality and safety of agricultural products. |
| format | Article |
| id | doaj-art-ac9d1291238c4dfbafdd21a23a8e7ac9 |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-ac9d1291238c4dfbafdd21a23a8e7ac92025-08-20T03:47:48ZengMDPI AGAgronomy2073-43952025-04-01155108410.3390/agronomy15051084Changes in Soil Properties, Content of Cd, and Cd-Resistant Bacterial Community with Biochar After One-Time Addition Under Soybean Continuous Cropping and Crop Rotation PatternsQin Yao0Gege He1Shubo Yan2Jie Song3Yuetong Zhang4Xiaoyu Zhang5Yan Sun6Yibo Wang7Yongjuan Li8Yu Tian9Changjiang Zhao10Yongxia Guo11College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163000, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163000, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163000, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163000, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163000, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163000, ChinaSchool of Resources and Environment, Northeast Agricultural University, Harbin 150030, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163000, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163000, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163000, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163000, ChinaCollege of Agriculture, Heilongjiang Bayi Agricultural University, Daqing 163000, ChinaIn recent years, Cadmium (Cd) pollution in soybean farmland is severe. Therefore, this study focused on whether biochar influences soil physiochemical properties, the Cd content in soil and soybean grains, and the abundance and community structure of the <i>czcA</i> gene. Four doses of rice husk biochar (0, 5, 15, and 25 t·ha<sup>−1</sup>) were applied under continuous cropping and crop rotation systems, and soil samples were collected after four years of one-time addition. The results indicated that biochar addition significantly increased soil available nitrogen, phosphorus, and soil organic carbon contents under continuous cropping and rotation. Biochar application significantly reduced the total Cd content of soil samples and soybean grains. Additionally, biochar application reduced <i>czcA</i> gene abundance in soybean soils by 14.26–37.88% and 35.96–48.71%, respectively. Correlation analysis revealed that Cd content and the abundance of the <i>czcA</i> gene significantly correlated with soil nutrients and pH. High-throughput sequencing revealed that the relative abundances of several Cd-resistant microorganisms were decreased by biochar addition. In addition, adding biochar significantly affected the Cd-resistant microbial community structure and diversity by influencing soil properties and Cd content. Therefore, this study has important practical significance for improving the soil environment and ensuring the quality and safety of agricultural products.https://www.mdpi.com/2073-4395/15/5/1084biocharCd resistanthigh-throughput sequencingmicrobial diversity |
| spellingShingle | Qin Yao Gege He Shubo Yan Jie Song Yuetong Zhang Xiaoyu Zhang Yan Sun Yibo Wang Yongjuan Li Yu Tian Changjiang Zhao Yongxia Guo Changes in Soil Properties, Content of Cd, and Cd-Resistant Bacterial Community with Biochar After One-Time Addition Under Soybean Continuous Cropping and Crop Rotation Patterns Agronomy biochar Cd resistant high-throughput sequencing microbial diversity |
| title | Changes in Soil Properties, Content of Cd, and Cd-Resistant Bacterial Community with Biochar After One-Time Addition Under Soybean Continuous Cropping and Crop Rotation Patterns |
| title_full | Changes in Soil Properties, Content of Cd, and Cd-Resistant Bacterial Community with Biochar After One-Time Addition Under Soybean Continuous Cropping and Crop Rotation Patterns |
| title_fullStr | Changes in Soil Properties, Content of Cd, and Cd-Resistant Bacterial Community with Biochar After One-Time Addition Under Soybean Continuous Cropping and Crop Rotation Patterns |
| title_full_unstemmed | Changes in Soil Properties, Content of Cd, and Cd-Resistant Bacterial Community with Biochar After One-Time Addition Under Soybean Continuous Cropping and Crop Rotation Patterns |
| title_short | Changes in Soil Properties, Content of Cd, and Cd-Resistant Bacterial Community with Biochar After One-Time Addition Under Soybean Continuous Cropping and Crop Rotation Patterns |
| title_sort | changes in soil properties content of cd and cd resistant bacterial community with biochar after one time addition under soybean continuous cropping and crop rotation patterns |
| topic | biochar Cd resistant high-throughput sequencing microbial diversity |
| url | https://www.mdpi.com/2073-4395/15/5/1084 |
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