Low-intensity pulsed ultrasound induces multifaced alterations in chromosome segregation, cytoskeletal filaments and cell junctions
Abstract Low-intensity pulsed ultrasound (LIPUS) is a widely used non-invasive approach with therapeutic purposes since it provides physical stimulation with minimal thermal effects. The skin epithelium is the first barrier of the human body that interfaces with LIPUS and is subjected to the highest...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-02-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-025-88569-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850067290466287616 |
|---|---|
| author | Ion Udroiu Federica Todaro Alessandra Vitaliti Damiano Palmieri Eugenia Guida Giulia Perilli Leonardo Duranti Cadia D’Ottavi Maurizio Mattei Susanna Dolci Gaio Paradossi Angelico Bedini Ida Silvestri Antonella Sgura Fabio Domenici |
| author_facet | Ion Udroiu Federica Todaro Alessandra Vitaliti Damiano Palmieri Eugenia Guida Giulia Perilli Leonardo Duranti Cadia D’Ottavi Maurizio Mattei Susanna Dolci Gaio Paradossi Angelico Bedini Ida Silvestri Antonella Sgura Fabio Domenici |
| author_sort | Ion Udroiu |
| collection | DOAJ |
| description | Abstract Low-intensity pulsed ultrasound (LIPUS) is a widely used non-invasive approach with therapeutic purposes since it provides physical stimulation with minimal thermal effects. The skin epithelium is the first barrier of the human body that interfaces with LIPUS and is subjected to the highest intensity. Little is known about the impact of LIPUS on the skin surface. This work investigates the biological effects of one-hour exposure to 1 MHz LIPUS on human keratinocytes HaCaT and tumoral SK-MEL-28 skin cells. Specifically, we evaluated the cellular state immediately after LIPUS treatment by analyzing cytogenetic endpoints and the response of cytoskeleton and cell junction proteins. Herein we demonstrate that LIPUS induces genomic damage as shown by an increase of chromosome malsegregation and a consequent decrease of cellular proliferation. The mechanical stimulus produced by LIPUS is also transmitted to the cytoskeletal compartment, inducing the expression and re-organization of junction proteins (i.e., E-cadherin and Desmosomes) and intermediate filaments (i.e., F-actin and Cytokeratins) with impact on cell morphology and cell adhesion. These in vitro results highlight the different outcomes following the cytogenetic damage and the resilience response exerted by the cytoskeleton upon mechanical stress, laying the foundation for future in vivo investigations. |
| format | Article |
| id | doaj-art-ac8cf8a11ba74fd09e6eda42dd8ddca9 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-ac8cf8a11ba74fd09e6eda42dd8ddca92025-08-20T02:48:22ZengNature PortfolioScientific Reports2045-23222025-02-0115111810.1038/s41598-025-88569-1Low-intensity pulsed ultrasound induces multifaced alterations in chromosome segregation, cytoskeletal filaments and cell junctionsIon Udroiu0Federica Todaro1Alessandra Vitaliti2Damiano Palmieri3Eugenia Guida4Giulia Perilli5Leonardo Duranti6Cadia D’Ottavi7Maurizio Mattei8Susanna Dolci9Gaio Paradossi10Angelico Bedini11Ida Silvestri12Antonella Sgura13Fabio Domenici14Department of Sciences, Università Roma TreSection of Endocrinology and Metabolic Diseases, Department of Systems Medicine, University of Rome “Tor Vergata”Department of Chemical Science and Technologies, University of Rome “Tor Vergata”Medical Physics Unit, Bambino Gesù Children’s Hospital, IRCCSDepartment of Biomedicine and Prevention, University Hospital of Rome “Tor Vergata”Department of Chemical Science and Technologies, University of Rome “Tor Vergata”Department of Chemical Science and Technologies, University of Rome “Tor Vergata”Department of Chemical Science and Technologies, University of Rome “Tor Vergata”Interdepartmental Center for Comparative Medicine, Alternative Techniques and Aquaculture (CIMETA), University of Rome “Tor Vergata”Department of Biomedicine and Prevention, University Hospital of Rome “Tor Vergata”Department of Chemical Science and Technologies, University of Rome “Tor Vergata”Department of Technological Innovations and Safety of Plants, Products and Anthropic Settlements (DIT), Italian National Institute for Insurance against Accidents at WorkDepartment of Molecular Medicine, Sapienza UniversityDepartment of Sciences, Università Roma TreDepartment of Chemical Science and Technologies, University of Rome “Tor Vergata”Abstract Low-intensity pulsed ultrasound (LIPUS) is a widely used non-invasive approach with therapeutic purposes since it provides physical stimulation with minimal thermal effects. The skin epithelium is the first barrier of the human body that interfaces with LIPUS and is subjected to the highest intensity. Little is known about the impact of LIPUS on the skin surface. This work investigates the biological effects of one-hour exposure to 1 MHz LIPUS on human keratinocytes HaCaT and tumoral SK-MEL-28 skin cells. Specifically, we evaluated the cellular state immediately after LIPUS treatment by analyzing cytogenetic endpoints and the response of cytoskeleton and cell junction proteins. Herein we demonstrate that LIPUS induces genomic damage as shown by an increase of chromosome malsegregation and a consequent decrease of cellular proliferation. The mechanical stimulus produced by LIPUS is also transmitted to the cytoskeletal compartment, inducing the expression and re-organization of junction proteins (i.e., E-cadherin and Desmosomes) and intermediate filaments (i.e., F-actin and Cytokeratins) with impact on cell morphology and cell adhesion. These in vitro results highlight the different outcomes following the cytogenetic damage and the resilience response exerted by the cytoskeleton upon mechanical stress, laying the foundation for future in vivo investigations.https://doi.org/10.1038/s41598-025-88569-1 |
| spellingShingle | Ion Udroiu Federica Todaro Alessandra Vitaliti Damiano Palmieri Eugenia Guida Giulia Perilli Leonardo Duranti Cadia D’Ottavi Maurizio Mattei Susanna Dolci Gaio Paradossi Angelico Bedini Ida Silvestri Antonella Sgura Fabio Domenici Low-intensity pulsed ultrasound induces multifaced alterations in chromosome segregation, cytoskeletal filaments and cell junctions Scientific Reports |
| title | Low-intensity pulsed ultrasound induces multifaced alterations in chromosome segregation, cytoskeletal filaments and cell junctions |
| title_full | Low-intensity pulsed ultrasound induces multifaced alterations in chromosome segregation, cytoskeletal filaments and cell junctions |
| title_fullStr | Low-intensity pulsed ultrasound induces multifaced alterations in chromosome segregation, cytoskeletal filaments and cell junctions |
| title_full_unstemmed | Low-intensity pulsed ultrasound induces multifaced alterations in chromosome segregation, cytoskeletal filaments and cell junctions |
| title_short | Low-intensity pulsed ultrasound induces multifaced alterations in chromosome segregation, cytoskeletal filaments and cell junctions |
| title_sort | low intensity pulsed ultrasound induces multifaced alterations in chromosome segregation cytoskeletal filaments and cell junctions |
| url | https://doi.org/10.1038/s41598-025-88569-1 |
| work_keys_str_mv | AT ionudroiu lowintensitypulsedultrasoundinducesmultifacedalterationsinchromosomesegregationcytoskeletalfilamentsandcelljunctions AT federicatodaro lowintensitypulsedultrasoundinducesmultifacedalterationsinchromosomesegregationcytoskeletalfilamentsandcelljunctions AT alessandravitaliti lowintensitypulsedultrasoundinducesmultifacedalterationsinchromosomesegregationcytoskeletalfilamentsandcelljunctions AT damianopalmieri lowintensitypulsedultrasoundinducesmultifacedalterationsinchromosomesegregationcytoskeletalfilamentsandcelljunctions AT eugeniaguida lowintensitypulsedultrasoundinducesmultifacedalterationsinchromosomesegregationcytoskeletalfilamentsandcelljunctions AT giuliaperilli lowintensitypulsedultrasoundinducesmultifacedalterationsinchromosomesegregationcytoskeletalfilamentsandcelljunctions AT leonardoduranti lowintensitypulsedultrasoundinducesmultifacedalterationsinchromosomesegregationcytoskeletalfilamentsandcelljunctions AT cadiadottavi lowintensitypulsedultrasoundinducesmultifacedalterationsinchromosomesegregationcytoskeletalfilamentsandcelljunctions AT mauriziomattei lowintensitypulsedultrasoundinducesmultifacedalterationsinchromosomesegregationcytoskeletalfilamentsandcelljunctions AT susannadolci lowintensitypulsedultrasoundinducesmultifacedalterationsinchromosomesegregationcytoskeletalfilamentsandcelljunctions AT gaioparadossi lowintensitypulsedultrasoundinducesmultifacedalterationsinchromosomesegregationcytoskeletalfilamentsandcelljunctions AT angelicobedini lowintensitypulsedultrasoundinducesmultifacedalterationsinchromosomesegregationcytoskeletalfilamentsandcelljunctions AT idasilvestri lowintensitypulsedultrasoundinducesmultifacedalterationsinchromosomesegregationcytoskeletalfilamentsandcelljunctions AT antonellasgura lowintensitypulsedultrasoundinducesmultifacedalterationsinchromosomesegregationcytoskeletalfilamentsandcelljunctions AT fabiodomenici lowintensitypulsedultrasoundinducesmultifacedalterationsinchromosomesegregationcytoskeletalfilamentsandcelljunctions |