Antimicrobial Assessment of Prunus spinosa L. Fruit Extracts
Prunus spinosa L., commonly known as blackthorn or sloe, is a member of the Rosaceae family that typically grows as a shrub on the slopes of uncultivated, wild terrain. In late autumn, fresh fruits were collected from three distinct habitats in Bosnia and Herzegovina: Borije, Vareš, and Trnovo. This...
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Croatian Society of Chemical Engineers
2025-07-01
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| Series: | Kemija u Industriji |
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| Online Access: | http://silverstripe.fkit.hr/kui/assets/Uploads/1-281-286-KUI-7-8-2025.pdf |
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| author | Alema Dedić Hurija Džudžević-Čančar Amra Alispahić Anesa Jerković-Mujkić |
| author_facet | Alema Dedić Hurija Džudžević-Čančar Amra Alispahić Anesa Jerković-Mujkić |
| author_sort | Alema Dedić |
| collection | DOAJ |
| description | Prunus spinosa L., commonly known as blackthorn or sloe, is a member of the Rosaceae family that typically grows as a shrub on the slopes of uncultivated, wild terrain. In late autumn, fresh fruits were collected from three distinct habitats in Bosnia and Herzegovina: Borije, Vareš, and Trnovo. This study investigated the antibacterial activity of fruit extracts prepared using the Soxhlet method, employing n-hexane, ethanol, diethyl ether, and dichloromethane. Antibacterial activity was assessed in vitro using the agar well diffusion method against standard bacterial strains: Gram-positive (Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29212), Gram-negative (Pseudomonas aeruginosa ATCC 9027, Salmonella enterica ATCC 31194, Escherichia coli ATCC 25922), and fungus (Candida albicans ATCC 1023). Four solvents served as negative controls, while the antibiotic streptomycin and the antimycotic nystatin served as positive controls. All the blackthorn extracts demonstrated varying degrees of antimicrobial activity against the bacterial strains tested. Ethanol fruit extracts from Borije, Trnovo, and Vareš exhibited the strongest antibacterial activity against the Gram-positive bacterial strain S. aureus, with inhibition zones measuring 21.00, 23.50, and 25.00 mm, respectively. Compared to the control sample, the extracts from Trnovo and Vareš displayed greater potency against this bacterial strain. The diethyl ether extract from Vareš was more effective than streptomycin against E. faecalis, exhibiting a highly sensitive inhibition zone of 22.00 mm. These results suggest that the blackthorn fruit extracts studied possess strong antibacterial activity. |
| format | Article |
| id | doaj-art-ac8b0b5648b74c4a8b7f3f8a28ad5732 |
| institution | DOAJ |
| issn | 0022-9830 1334-9090 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Croatian Society of Chemical Engineers |
| record_format | Article |
| series | Kemija u Industriji |
| spelling | doaj-art-ac8b0b5648b74c4a8b7f3f8a28ad57322025-08-20T03:12:02ZengCroatian Society of Chemical EngineersKemija u Industriji0022-98301334-90902025-07-01747-828128610.15255/KUI.2024.050Antimicrobial Assessment of Prunus spinosa L. Fruit ExtractsAlema Dedić0Hurija Džudžević-Čančar1Amra Alispahić2Anesa Jerković-Mujkić3University of Sarajevo, University of Sarajevo – Faculty of Pharmacy, Department of Chemistry in Pharmacy, Zmaja od Bosne 8, 71 000 Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo, University of Sarajevo – Faculty of Pharmacy, Department of Chemistry in Pharmacy, Zmaja od Bosne 8, 71 000 Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo, University of Sarajevo – Faculty of Pharmacy, Department of Chemistry in Pharmacy, Zmaja od Bosne 8, 71 000 Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo, University of Sarajevo – Faculty of Science, Department of Biology, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and HerzegovinaPrunus spinosa L., commonly known as blackthorn or sloe, is a member of the Rosaceae family that typically grows as a shrub on the slopes of uncultivated, wild terrain. In late autumn, fresh fruits were collected from three distinct habitats in Bosnia and Herzegovina: Borije, Vareš, and Trnovo. This study investigated the antibacterial activity of fruit extracts prepared using the Soxhlet method, employing n-hexane, ethanol, diethyl ether, and dichloromethane. Antibacterial activity was assessed in vitro using the agar well diffusion method against standard bacterial strains: Gram-positive (Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, Enterococcus faecalis ATCC 29212), Gram-negative (Pseudomonas aeruginosa ATCC 9027, Salmonella enterica ATCC 31194, Escherichia coli ATCC 25922), and fungus (Candida albicans ATCC 1023). Four solvents served as negative controls, while the antibiotic streptomycin and the antimycotic nystatin served as positive controls. All the blackthorn extracts demonstrated varying degrees of antimicrobial activity against the bacterial strains tested. Ethanol fruit extracts from Borije, Trnovo, and Vareš exhibited the strongest antibacterial activity against the Gram-positive bacterial strain S. aureus, with inhibition zones measuring 21.00, 23.50, and 25.00 mm, respectively. Compared to the control sample, the extracts from Trnovo and Vareš displayed greater potency against this bacterial strain. The diethyl ether extract from Vareš was more effective than streptomycin against E. faecalis, exhibiting a highly sensitive inhibition zone of 22.00 mm. These results suggest that the blackthorn fruit extracts studied possess strong antibacterial activity. http://silverstripe.fkit.hr/kui/assets/Uploads/1-281-286-KUI-7-8-2025.pdfprunus spinosa l.antimicrobial activitysoxhlet extractionfruit extracts |
| spellingShingle | Alema Dedić Hurija Džudžević-Čančar Amra Alispahić Anesa Jerković-Mujkić Antimicrobial Assessment of Prunus spinosa L. Fruit Extracts Kemija u Industriji prunus spinosa l. antimicrobial activity soxhlet extraction fruit extracts |
| title | Antimicrobial Assessment of Prunus spinosa L. Fruit Extracts |
| title_full | Antimicrobial Assessment of Prunus spinosa L. Fruit Extracts |
| title_fullStr | Antimicrobial Assessment of Prunus spinosa L. Fruit Extracts |
| title_full_unstemmed | Antimicrobial Assessment of Prunus spinosa L. Fruit Extracts |
| title_short | Antimicrobial Assessment of Prunus spinosa L. Fruit Extracts |
| title_sort | antimicrobial assessment of prunus spinosa l fruit extracts |
| topic | prunus spinosa l. antimicrobial activity soxhlet extraction fruit extracts |
| url | http://silverstripe.fkit.hr/kui/assets/Uploads/1-281-286-KUI-7-8-2025.pdf |
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