SERS-based quantitative vertical flow assay using dual Au-Ag SERS nanoprobes
A surface-enhanced Raman scattering (SERS)-based vertical flow assay (VFA) platform was developed for the point-of-care testing (POCT) to perform multiplex diagnosis and distinguish each disease with similar symptoms (e.g., analysis of respiratory diseases including SARS-CoV-2, influenza and RSV). T...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Results in Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715624006362 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832583110880919552 |
|---|---|
| author | Jinhyeok Jeon Ji-In Park Chulhyun Lee |
| author_facet | Jinhyeok Jeon Ji-In Park Chulhyun Lee |
| author_sort | Jinhyeok Jeon |
| collection | DOAJ |
| description | A surface-enhanced Raman scattering (SERS)-based vertical flow assay (VFA) platform was developed for the point-of-care testing (POCT) to perform multiplex diagnosis and distinguish each disease with similar symptoms (e.g., analysis of respiratory diseases including SARS-CoV-2, influenza and RSV). To evaluate the feasibility of multiplexing performance of SERS-VFA platform, vertical flow cartridges with 2 × 2 test areas on the nitrocellulose membrane and two different Raman reporter-labeled SERS nanoprobes were prepared, to detect samples including two different immunoglobulins (mouse IgG and rabbit IgG). For the preparation of SERS nanoprobes, we made use of the gold-silver/core–shell metal nanostructures for the local electromagnetic-field enhancement effect, and the Raman signal could be improved. SERS mapping images for each IgG in the sample were generated by measuring Raman peak position originated from each individual SERS nanoprobes. As a result, the quantitative analysis of each individual IgG could be performed at once, with a single sample. The detection limit for each rabbit IgG and mouse IgG was calculated as 0.030 μg/mL and 0.18 μg/mL, respectively. This result shows the SERS-based VFA platform could be of significant utility in multiplexed detection of chemical or biological targets. |
| format | Article |
| id | doaj-art-ac85e0b29e214e108938e95bfbd194eb |
| institution | Kabale University |
| issn | 2211-7156 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj-art-ac85e0b29e214e108938e95bfbd194eb2025-01-29T05:00:38ZengElsevierResults in Chemistry2211-71562025-01-0113101940SERS-based quantitative vertical flow assay using dual Au-Ag SERS nanoprobesJinhyeok Jeon0Ji-In Park1Chulhyun Lee2Department of Engineering Support, Korea Basic Science Institute, Daejeon 34133, South Korea; Corresponding author.Research Center for Materials Analysis, Korea Basic Science Institute, Daejeon 34133, South KoreaDepartment of Engineering Support, Korea Basic Science Institute, Daejeon 34133, South KoreaA surface-enhanced Raman scattering (SERS)-based vertical flow assay (VFA) platform was developed for the point-of-care testing (POCT) to perform multiplex diagnosis and distinguish each disease with similar symptoms (e.g., analysis of respiratory diseases including SARS-CoV-2, influenza and RSV). To evaluate the feasibility of multiplexing performance of SERS-VFA platform, vertical flow cartridges with 2 × 2 test areas on the nitrocellulose membrane and two different Raman reporter-labeled SERS nanoprobes were prepared, to detect samples including two different immunoglobulins (mouse IgG and rabbit IgG). For the preparation of SERS nanoprobes, we made use of the gold-silver/core–shell metal nanostructures for the local electromagnetic-field enhancement effect, and the Raman signal could be improved. SERS mapping images for each IgG in the sample were generated by measuring Raman peak position originated from each individual SERS nanoprobes. As a result, the quantitative analysis of each individual IgG could be performed at once, with a single sample. The detection limit for each rabbit IgG and mouse IgG was calculated as 0.030 μg/mL and 0.18 μg/mL, respectively. This result shows the SERS-based VFA platform could be of significant utility in multiplexed detection of chemical or biological targets.http://www.sciencedirect.com/science/article/pii/S2211715624006362Surface-enhanced Raman scattering (SERS)Raman mappingVertical flow assay (VFA)Dual Au-Ag SERS probesPoint-of-care testing (POCT) |
| spellingShingle | Jinhyeok Jeon Ji-In Park Chulhyun Lee SERS-based quantitative vertical flow assay using dual Au-Ag SERS nanoprobes Results in Chemistry Surface-enhanced Raman scattering (SERS) Raman mapping Vertical flow assay (VFA) Dual Au-Ag SERS probes Point-of-care testing (POCT) |
| title | SERS-based quantitative vertical flow assay using dual Au-Ag SERS nanoprobes |
| title_full | SERS-based quantitative vertical flow assay using dual Au-Ag SERS nanoprobes |
| title_fullStr | SERS-based quantitative vertical flow assay using dual Au-Ag SERS nanoprobes |
| title_full_unstemmed | SERS-based quantitative vertical flow assay using dual Au-Ag SERS nanoprobes |
| title_short | SERS-based quantitative vertical flow assay using dual Au-Ag SERS nanoprobes |
| title_sort | sers based quantitative vertical flow assay using dual au ag sers nanoprobes |
| topic | Surface-enhanced Raman scattering (SERS) Raman mapping Vertical flow assay (VFA) Dual Au-Ag SERS probes Point-of-care testing (POCT) |
| url | http://www.sciencedirect.com/science/article/pii/S2211715624006362 |
| work_keys_str_mv | AT jinhyeokjeon sersbasedquantitativeverticalflowassayusingdualauagsersnanoprobes AT jiinpark sersbasedquantitativeverticalflowassayusingdualauagsersnanoprobes AT chulhyunlee sersbasedquantitativeverticalflowassayusingdualauagsersnanoprobes |