A Comparative Study on Drought Stress Response In Vitro and In Vivo Propagated <i>Fragaria vesca</i> Plants
The evaluation of plant responses to water deficit in the substrate, particularly in the context of intensifying climate change, represents a critical factor for ensuring stable agricultural production, economic resilience, and food security. The primary objective of this study was to compare the ph...
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2025-01-01
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| author | Marta Rokosa Małgorzata Mikiciuk Danuta Kulpa Piotr Ptak |
| author_facet | Marta Rokosa Małgorzata Mikiciuk Danuta Kulpa Piotr Ptak |
| author_sort | Marta Rokosa |
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| description | The evaluation of plant responses to water deficit in the substrate, particularly in the context of intensifying climate change, represents a critical factor for ensuring stable agricultural production, economic resilience, and food security. The primary objective of this study was to compare the physiological and biochemical responses to water deficit in conventional cultivation of <i>Fragaria vesca</i> plants propagated both in vitro and in vivo. The research encompassed measurements of gas exchange parameters, chlorophyll “a” fluorescence, photosynthetic pigment and proline content in leaves, leaf relative water content index, total fruit yield, single fruit weight, fresh and dry mass of the root system, as well as the concentrations of K, Ca, Mg, Na, Cu, Zn, Mn, and Mo. Additionally, the ratio of monovalent to divalent cations in leaves, roots, and crowns was analyzed. The results revealed significant differences between the experimental variants under optimal conditions and their respective responses to drought stress. Plants derived from in vitro cultures, despite exhibiting initially lower physiological trait values, demonstrated higher yield potential (no significant difference in the yield of fresh fruit mass compared to a 78% reduction). However, a long-term lack of water caused greater damage to their photosynthetic system—a reduction in physiological traits to 80% was observed, compared to a maximum decrease of 40% in plants derived from seedlings. The results highlight that environmental conditions and the acclimatization process of plants derived from in vitro cultures can significantly influence their adaptive potential and productivity. |
| format | Article |
| id | doaj-art-ac7ce12f1bad4beb94d35e1e6ef0e436 |
| institution | Kabale University |
| issn | 2077-0472 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Agriculture |
| spelling | doaj-art-ac7ce12f1bad4beb94d35e1e6ef0e4362025-01-24T13:15:53ZengMDPI AGAgriculture2077-04722025-01-0115214510.3390/agriculture15020145A Comparative Study on Drought Stress Response In Vitro and In Vivo Propagated <i>Fragaria vesca</i> PlantsMarta Rokosa0Małgorzata Mikiciuk1Danuta Kulpa2Piotr Ptak3Department of Bioengineering, Faculty of Environmental Management and Agriculture, West Pomeranian University of Technology, Słowackiego 17, 71-434 Szczecin, PolandDepartment of Bioengineering, Faculty of Environmental Management and Agriculture, West Pomeranian University of Technology, Słowackiego 17, 71-434 Szczecin, PolandDepartment of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Słowackiego 17, 71-434 Szczecin, PolandDepartment of Bioengineering, Faculty of Environmental Management and Agriculture, West Pomeranian University of Technology, Słowackiego 17, 71-434 Szczecin, PolandThe evaluation of plant responses to water deficit in the substrate, particularly in the context of intensifying climate change, represents a critical factor for ensuring stable agricultural production, economic resilience, and food security. The primary objective of this study was to compare the physiological and biochemical responses to water deficit in conventional cultivation of <i>Fragaria vesca</i> plants propagated both in vitro and in vivo. The research encompassed measurements of gas exchange parameters, chlorophyll “a” fluorescence, photosynthetic pigment and proline content in leaves, leaf relative water content index, total fruit yield, single fruit weight, fresh and dry mass of the root system, as well as the concentrations of K, Ca, Mg, Na, Cu, Zn, Mn, and Mo. Additionally, the ratio of monovalent to divalent cations in leaves, roots, and crowns was analyzed. The results revealed significant differences between the experimental variants under optimal conditions and their respective responses to drought stress. Plants derived from in vitro cultures, despite exhibiting initially lower physiological trait values, demonstrated higher yield potential (no significant difference in the yield of fresh fruit mass compared to a 78% reduction). However, a long-term lack of water caused greater damage to their photosynthetic system—a reduction in physiological traits to 80% was observed, compared to a maximum decrease of 40% in plants derived from seedlings. The results highlight that environmental conditions and the acclimatization process of plants derived from in vitro cultures can significantly influence their adaptive potential and productivity.https://www.mdpi.com/2077-0472/15/2/145<i>Fragaria vesca</i>droughtchlorophyll fluorescencegas exchangechemical compositionin vitro cultures |
| spellingShingle | Marta Rokosa Małgorzata Mikiciuk Danuta Kulpa Piotr Ptak A Comparative Study on Drought Stress Response In Vitro and In Vivo Propagated <i>Fragaria vesca</i> Plants Agriculture <i>Fragaria vesca</i> drought chlorophyll fluorescence gas exchange chemical composition in vitro cultures |
| title | A Comparative Study on Drought Stress Response In Vitro and In Vivo Propagated <i>Fragaria vesca</i> Plants |
| title_full | A Comparative Study on Drought Stress Response In Vitro and In Vivo Propagated <i>Fragaria vesca</i> Plants |
| title_fullStr | A Comparative Study on Drought Stress Response In Vitro and In Vivo Propagated <i>Fragaria vesca</i> Plants |
| title_full_unstemmed | A Comparative Study on Drought Stress Response In Vitro and In Vivo Propagated <i>Fragaria vesca</i> Plants |
| title_short | A Comparative Study on Drought Stress Response In Vitro and In Vivo Propagated <i>Fragaria vesca</i> Plants |
| title_sort | comparative study on drought stress response in vitro and in vivo propagated i fragaria vesca i plants |
| topic | <i>Fragaria vesca</i> drought chlorophyll fluorescence gas exchange chemical composition in vitro cultures |
| url | https://www.mdpi.com/2077-0472/15/2/145 |
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