Characterization and comparison of fecal microbiota in horses with pituitary pars intermedia dysfunction and age‐matched controls
Abstract Background Altered gut microbiota has been associated with dopaminergic degenerative diseases in people, but studies on horses with pituitary pars intermedia dysfunction (PPID) are lacking. Hypothesis/Objectives Investigate the effect of PPID on fecal microbiota in horses. Animals Nine hors...
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| Format: | Article |
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Wiley
2025-01-01
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| Series: | Journal of Veterinary Internal Medicine |
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| Online Access: | https://doi.org/10.1111/jvim.17288 |
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| author | Wenqing Wang Justine Gibson Sara Horsman Deirdre Mikkelsen François‐René Bertin |
| author_facet | Wenqing Wang Justine Gibson Sara Horsman Deirdre Mikkelsen François‐René Bertin |
| author_sort | Wenqing Wang |
| collection | DOAJ |
| description | Abstract Background Altered gut microbiota has been associated with dopaminergic degenerative diseases in people, but studies on horses with pituitary pars intermedia dysfunction (PPID) are lacking. Hypothesis/Objectives Investigate the effect of PPID on fecal microbiota in horses. Animals Nine horses with PPID and 13 age‐matched control horses. Methods Prospective control study. Fecal samples were collected bimonthly. Microbial analysis used 16S rRNA sequencing to determine the relative abundance at genus and phylum levels, assess alpha and beta diversity and identify core microbiota. Results Horses with PPID had decreased relative abundances of Christensenellaceae R‐7 group (median; 95% confidence interval [CI]: PPID, 2.04; 1.82‐2.35 vs control, 2.54; 2.37‐2.76; P = .02) and NK4A214 group (PPID, 2.21; 2.02‐2.56 vs control, 2.62; 2.44‐2.85; P = .05), and significant lower abundances of Romboutsia (log2FoldChange = −3.54; P = .04) and Peptococcaceae uncultured (log2FoldChange = −0.89; P = .04) by differential abundance analysis. However, the abundance of Fibrobacter (log2FoldChange = 0.74; P = .04) was significantly higher in the PPID group. A significant effect of PPID on beta diversity was observed (P = .004), whereas alpha diversity varied with months (P = .001). Seven unique genera were identified in horses with PPID and 12 in control horses. Conclusions and Clinical Importance The fecal microbial composition is altered in horses with PPID. These findings support the potential role of the microbiota‐gut‐brain axis in the pathogenesis of PPID. |
| format | Article |
| id | doaj-art-ac7aeeaff394477b98f9938215f0b033 |
| institution | Kabale University |
| issn | 0891-6640 1939-1676 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj-art-ac7aeeaff394477b98f9938215f0b0332025-01-27T15:22:41ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762025-01-01391n/an/a10.1111/jvim.17288Characterization and comparison of fecal microbiota in horses with pituitary pars intermedia dysfunction and age‐matched controlsWenqing Wang0Justine Gibson1Sara Horsman2Deirdre Mikkelsen3François‐René Bertin4School of Veterinary Science The University of Queensland Gatton Queensland AustraliaSchool of Veterinary Science The University of Queensland Gatton Queensland AustraliaSchool of Veterinary Science The University of Queensland Gatton Queensland AustraliaSchool of Agriculture and Food Sciences The University of Queensland St Lucia Queensland AustraliaSchool of Veterinary Science The University of Queensland Gatton Queensland AustraliaAbstract Background Altered gut microbiota has been associated with dopaminergic degenerative diseases in people, but studies on horses with pituitary pars intermedia dysfunction (PPID) are lacking. Hypothesis/Objectives Investigate the effect of PPID on fecal microbiota in horses. Animals Nine horses with PPID and 13 age‐matched control horses. Methods Prospective control study. Fecal samples were collected bimonthly. Microbial analysis used 16S rRNA sequencing to determine the relative abundance at genus and phylum levels, assess alpha and beta diversity and identify core microbiota. Results Horses with PPID had decreased relative abundances of Christensenellaceae R‐7 group (median; 95% confidence interval [CI]: PPID, 2.04; 1.82‐2.35 vs control, 2.54; 2.37‐2.76; P = .02) and NK4A214 group (PPID, 2.21; 2.02‐2.56 vs control, 2.62; 2.44‐2.85; P = .05), and significant lower abundances of Romboutsia (log2FoldChange = −3.54; P = .04) and Peptococcaceae uncultured (log2FoldChange = −0.89; P = .04) by differential abundance analysis. However, the abundance of Fibrobacter (log2FoldChange = 0.74; P = .04) was significantly higher in the PPID group. A significant effect of PPID on beta diversity was observed (P = .004), whereas alpha diversity varied with months (P = .001). Seven unique genera were identified in horses with PPID and 12 in control horses. Conclusions and Clinical Importance The fecal microbial composition is altered in horses with PPID. These findings support the potential role of the microbiota‐gut‐brain axis in the pathogenesis of PPID.https://doi.org/10.1111/jvim.1728816S rRNAACTHdegenerative diseasedopamineendocrinology |
| spellingShingle | Wenqing Wang Justine Gibson Sara Horsman Deirdre Mikkelsen François‐René Bertin Characterization and comparison of fecal microbiota in horses with pituitary pars intermedia dysfunction and age‐matched controls Journal of Veterinary Internal Medicine 16S rRNA ACTH degenerative disease dopamine endocrinology |
| title | Characterization and comparison of fecal microbiota in horses with pituitary pars intermedia dysfunction and age‐matched controls |
| title_full | Characterization and comparison of fecal microbiota in horses with pituitary pars intermedia dysfunction and age‐matched controls |
| title_fullStr | Characterization and comparison of fecal microbiota in horses with pituitary pars intermedia dysfunction and age‐matched controls |
| title_full_unstemmed | Characterization and comparison of fecal microbiota in horses with pituitary pars intermedia dysfunction and age‐matched controls |
| title_short | Characterization and comparison of fecal microbiota in horses with pituitary pars intermedia dysfunction and age‐matched controls |
| title_sort | characterization and comparison of fecal microbiota in horses with pituitary pars intermedia dysfunction and age matched controls |
| topic | 16S rRNA ACTH degenerative disease dopamine endocrinology |
| url | https://doi.org/10.1111/jvim.17288 |
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