<i>Bifidobacterium animalis</i> Supplementation Improves Intestinal Barrier Function and Alleviates Antibiotic-Associated Diarrhea in Mice
Probiotics have gained increasing recognition for their potential to mitigate antibiotic-associated diarrhea (AAD). However, the precise mechanisms underlying their effects remain unclear. This study developed a mouse model of AAD using ceftriaxone to investigate the alleviating effects and mechanis...
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MDPI AG
2025-05-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/14/10/1704 |
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| author | Xiaoyu Du Mingkun Liu Jingyu Li Yue Liu Shaoyang Ge Haina Gao Ming Zhang |
| author_facet | Xiaoyu Du Mingkun Liu Jingyu Li Yue Liu Shaoyang Ge Haina Gao Ming Zhang |
| author_sort | Xiaoyu Du |
| collection | DOAJ |
| description | Probiotics have gained increasing recognition for their potential to mitigate antibiotic-associated diarrhea (AAD). However, the precise mechanisms underlying their effects remain unclear. This study developed a mouse model of AAD using ceftriaxone to investigate the alleviating effects and mechanisms of <i>Bifidobacterium animalis</i> A6 (A6). The findings indicated that A6 supplementation effectively attenuated ceftriaxone-associated diarrhea in mice. The morphological damage to the villi and crypts was partially restored and more neatly reorganized following the A6 intervention. Additionally, intestinal morphology observations revealed a significant increase in the thickness of the mucus layer in the A6-treated group. Further examination of key regulatory genes associated with mucus secretion demonstrated that the A6 intervention effectively upregulated the expression of <i>mucin1</i>, thereby reinforcing the mucus layer. Concurrently, the A6 intervention upregulated the expression of the <i>AQP4</i> and <i>SLC26A3</i> genes in the intestine, which is responsible for restoring water absorption capacity in AAD mice. Additionally, the A6 treatment reduced ceftriaxone-induced harm to the intestinal microbiota of the mice, boosting beneficial bacteria like <i>Bacteroidales</i>, <i>Akkermansia</i>, <i>Bifidobacterium</i>, and <i>Lactobacillus</i>. Overall, this study offers valuable insights into the potential therapeutic role of A6 in restoring intestinal homeostasis and alleviating symptoms associated with AAD. |
| format | Article |
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| institution | DOAJ |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-05-01 |
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| series | Foods |
| spelling | doaj-art-ac752c2e11884de4834bbd1c5817f4e62025-08-20T03:14:35ZengMDPI AGFoods2304-81582025-05-011410170410.3390/foods14101704<i>Bifidobacterium animalis</i> Supplementation Improves Intestinal Barrier Function and Alleviates Antibiotic-Associated Diarrhea in MiceXiaoyu Du0Mingkun Liu1Jingyu Li2Yue Liu3Shaoyang Ge4Haina Gao5Ming Zhang6School of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaSchool of Food and Biological Engineering, Hefei University of Technology, Hefei 230000, ChinaSchool of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaSchool of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaSchool of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaSchool of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaSchool of Food and Health, Beijing Technology and Business University, Beijing 100048, ChinaProbiotics have gained increasing recognition for their potential to mitigate antibiotic-associated diarrhea (AAD). However, the precise mechanisms underlying their effects remain unclear. This study developed a mouse model of AAD using ceftriaxone to investigate the alleviating effects and mechanisms of <i>Bifidobacterium animalis</i> A6 (A6). The findings indicated that A6 supplementation effectively attenuated ceftriaxone-associated diarrhea in mice. The morphological damage to the villi and crypts was partially restored and more neatly reorganized following the A6 intervention. Additionally, intestinal morphology observations revealed a significant increase in the thickness of the mucus layer in the A6-treated group. Further examination of key regulatory genes associated with mucus secretion demonstrated that the A6 intervention effectively upregulated the expression of <i>mucin1</i>, thereby reinforcing the mucus layer. Concurrently, the A6 intervention upregulated the expression of the <i>AQP4</i> and <i>SLC26A3</i> genes in the intestine, which is responsible for restoring water absorption capacity in AAD mice. Additionally, the A6 treatment reduced ceftriaxone-induced harm to the intestinal microbiota of the mice, boosting beneficial bacteria like <i>Bacteroidales</i>, <i>Akkermansia</i>, <i>Bifidobacterium</i>, and <i>Lactobacillus</i>. Overall, this study offers valuable insights into the potential therapeutic role of A6 in restoring intestinal homeostasis and alleviating symptoms associated with AAD.https://www.mdpi.com/2304-8158/14/10/1704antibiotic-associated diarrhea<i>Bifidobacterium animalis</i>intestinal mucosal barriermicrobiota |
| spellingShingle | Xiaoyu Du Mingkun Liu Jingyu Li Yue Liu Shaoyang Ge Haina Gao Ming Zhang <i>Bifidobacterium animalis</i> Supplementation Improves Intestinal Barrier Function and Alleviates Antibiotic-Associated Diarrhea in Mice Foods antibiotic-associated diarrhea <i>Bifidobacterium animalis</i> intestinal mucosal barrier microbiota |
| title | <i>Bifidobacterium animalis</i> Supplementation Improves Intestinal Barrier Function and Alleviates Antibiotic-Associated Diarrhea in Mice |
| title_full | <i>Bifidobacterium animalis</i> Supplementation Improves Intestinal Barrier Function and Alleviates Antibiotic-Associated Diarrhea in Mice |
| title_fullStr | <i>Bifidobacterium animalis</i> Supplementation Improves Intestinal Barrier Function and Alleviates Antibiotic-Associated Diarrhea in Mice |
| title_full_unstemmed | <i>Bifidobacterium animalis</i> Supplementation Improves Intestinal Barrier Function and Alleviates Antibiotic-Associated Diarrhea in Mice |
| title_short | <i>Bifidobacterium animalis</i> Supplementation Improves Intestinal Barrier Function and Alleviates Antibiotic-Associated Diarrhea in Mice |
| title_sort | i bifidobacterium animalis i supplementation improves intestinal barrier function and alleviates antibiotic associated diarrhea in mice |
| topic | antibiotic-associated diarrhea <i>Bifidobacterium animalis</i> intestinal mucosal barrier microbiota |
| url | https://www.mdpi.com/2304-8158/14/10/1704 |
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