The Effects of Tempol on Cyclophosphamide-Induced Oxidative Stress in Rat Micturition Reflexes
We hypothesized that cyclophosphamide- (CYP-) induced cystitis results in oxidative stress and contributes to urinary bladder dysfunction. We determined (1) the expression of oxidative stress markers 3-nitrotyrosine (3-NT), reactive oxygen species (ROS)/reactive nitrogen species (RNS), inflammatory...
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| Format: | Article |
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Wiley
2015-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2015/545048 |
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| author | Eric J. Gonzalez Abbey Peterson Susan Malley Mitchel Daniel Daniel Lambert Michael Kosofsky Margaret A. Vizzard |
| author_facet | Eric J. Gonzalez Abbey Peterson Susan Malley Mitchel Daniel Daniel Lambert Michael Kosofsky Margaret A. Vizzard |
| author_sort | Eric J. Gonzalez |
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| description | We hypothesized that cyclophosphamide- (CYP-) induced cystitis results in oxidative stress and contributes to urinary bladder dysfunction. We determined (1) the expression of oxidative stress markers 3-nitrotyrosine (3-NT), reactive oxygen species (ROS)/reactive nitrogen species (RNS), inflammatory modulators, neuropeptides calcitonin gene-related peptide (CGRP), substance P (Sub P), and adenosine triphosphate (ATP) that contribute to the inflammatory process in the urinary tract and (2) the functional role of oxidative stress in urinary bladder dysfunction with an antioxidant, Tempol, (1 mM in drinking water) combined with conscious cystometry. In CYP-treated (4 hr or 48 hr; 150 mg/kg, i.p.) rats, ROS/RNS and 3-NT significantly (P≤0.01) increased in urinary bladder. CYP treatment increased ATP, Sub P, and CGRP expression in the urinary bladder and cystometric fluid. In CYP-treated rats, Tempol significantly (P≤0.01) increased bladder capacity and reduced voiding frequency compared to CYP-treated rats without Tempol. Tempol significantly (P≤0.01) reduced ATP expression, 3-NT, and ROS/RNS expression in the urinary tract of CYP-treated rats. These studies demonstrate that reducing oxidative stress in CYP-induced cystitis improves urinary bladder function and reduces markers of oxidative stress and inflammation. |
| format | Article |
| id | doaj-art-ac6a9382fa884d608a2b81fb0ad3bc27 |
| institution | OA Journals |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-ac6a9382fa884d608a2b81fb0ad3bc272025-08-20T02:20:22ZengWileyThe Scientific World Journal2356-61401537-744X2015-01-01201510.1155/2015/545048545048The Effects of Tempol on Cyclophosphamide-Induced Oxidative Stress in Rat Micturition ReflexesEric J. Gonzalez0Abbey Peterson1Susan Malley2Mitchel Daniel3Daniel Lambert4Michael Kosofsky5Margaret A. Vizzard6Department of Neurological Sciences, University of Vermont College of Medicine, Burlington, VT 05405, USADepartment of Neurological Sciences, University of Vermont College of Medicine, Burlington, VT 05405, USADepartment of Neurological Sciences, University of Vermont College of Medicine, Burlington, VT 05405, USADepartment of Neurological Sciences, University of Vermont College of Medicine, Burlington, VT 05405, USADepartment of Neurological Sciences, University of Vermont College of Medicine, Burlington, VT 05405, USADepartment of Neurological Sciences, University of Vermont College of Medicine, Burlington, VT 05405, USADepartment of Neurological Sciences, University of Vermont College of Medicine, Burlington, VT 05405, USAWe hypothesized that cyclophosphamide- (CYP-) induced cystitis results in oxidative stress and contributes to urinary bladder dysfunction. We determined (1) the expression of oxidative stress markers 3-nitrotyrosine (3-NT), reactive oxygen species (ROS)/reactive nitrogen species (RNS), inflammatory modulators, neuropeptides calcitonin gene-related peptide (CGRP), substance P (Sub P), and adenosine triphosphate (ATP) that contribute to the inflammatory process in the urinary tract and (2) the functional role of oxidative stress in urinary bladder dysfunction with an antioxidant, Tempol, (1 mM in drinking water) combined with conscious cystometry. In CYP-treated (4 hr or 48 hr; 150 mg/kg, i.p.) rats, ROS/RNS and 3-NT significantly (P≤0.01) increased in urinary bladder. CYP treatment increased ATP, Sub P, and CGRP expression in the urinary bladder and cystometric fluid. In CYP-treated rats, Tempol significantly (P≤0.01) increased bladder capacity and reduced voiding frequency compared to CYP-treated rats without Tempol. Tempol significantly (P≤0.01) reduced ATP expression, 3-NT, and ROS/RNS expression in the urinary tract of CYP-treated rats. These studies demonstrate that reducing oxidative stress in CYP-induced cystitis improves urinary bladder function and reduces markers of oxidative stress and inflammation.http://dx.doi.org/10.1155/2015/545048 |
| spellingShingle | Eric J. Gonzalez Abbey Peterson Susan Malley Mitchel Daniel Daniel Lambert Michael Kosofsky Margaret A. Vizzard The Effects of Tempol on Cyclophosphamide-Induced Oxidative Stress in Rat Micturition Reflexes The Scientific World Journal |
| title | The Effects of Tempol on Cyclophosphamide-Induced Oxidative Stress in Rat Micturition Reflexes |
| title_full | The Effects of Tempol on Cyclophosphamide-Induced Oxidative Stress in Rat Micturition Reflexes |
| title_fullStr | The Effects of Tempol on Cyclophosphamide-Induced Oxidative Stress in Rat Micturition Reflexes |
| title_full_unstemmed | The Effects of Tempol on Cyclophosphamide-Induced Oxidative Stress in Rat Micturition Reflexes |
| title_short | The Effects of Tempol on Cyclophosphamide-Induced Oxidative Stress in Rat Micturition Reflexes |
| title_sort | effects of tempol on cyclophosphamide induced oxidative stress in rat micturition reflexes |
| url | http://dx.doi.org/10.1155/2015/545048 |
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