Efficient utilization of Shuanghuanglian medicine residues by microbial transformation with flavonoid glycosides-hydrolyzing strains
Shuanghuanglian, a traditional Chinese medicine, is well-known for its bioactive compounds, such as flavonoids, which offer significant health benefits. However, the production of Shuanghuanlian generates substantial pharmaceutical residues, which are often discarded as waste, posing significant env...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1553399/full |
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| author | Xingwang Ma Xingwang Ma Ying Chen Lili Li Lili Li Tianwei Wang Kunling Teng Junchang Su Junchang Su Lei Li Liangliang Li Heping Li Wentao Diao Guocan Chen Jiwen Wang Jin Zhong Jin Zhong |
| author_facet | Xingwang Ma Xingwang Ma Ying Chen Lili Li Lili Li Tianwei Wang Kunling Teng Junchang Su Junchang Su Lei Li Liangliang Li Heping Li Wentao Diao Guocan Chen Jiwen Wang Jin Zhong Jin Zhong |
| author_sort | Xingwang Ma |
| collection | DOAJ |
| description | Shuanghuanglian, a traditional Chinese medicine, is well-known for its bioactive compounds, such as flavonoids, which offer significant health benefits. However, the production of Shuanghuanlian generates substantial pharmaceutical residues, which are often discarded as waste, posing significant environmental and economic challenges. To date, research on repurposing these medicine residues has been limited. This study utilized beneficial microbes to efficiently extract and utilize the residual bioactive compounds. Notably, a newly isolated Lactiplantibacillus plantarum strain LLB exhibited remarkable efficiency in converting flavonoid glycosides (e.g., phillyrin and luteoloside) into their corresponding aglycones. When combined synergistically with Bacillus subtilis and Saccharomyces cerevisiae, strain LLB maintained robust flavonoid glycoside conversion while enhancing lactobacilli viability in the fermented medicine residues. As a feed additive for broiler chickens, the fermented residue not only boosted antioxidant (superoxide dismutase) and anti-inflammatory (IL-10) markers, but also preserved growth performance and meat quality. Furthermore, the fermented residue modulated the gut microbiome, increasing Rikenella while reducing Elusimicrobiota and Parabacteroides abundances. Our findings demonstrate that microbial transformation of Shuanghuanglian residues offers a sustainable strategy for waste valorization and a novel feed additive for enhancing animal health. |
| format | Article |
| id | doaj-art-ac5902b7b629492aa2db9fcf728ef0e0 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-ac5902b7b629492aa2db9fcf728ef0e02025-08-20T03:48:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-05-011610.3389/fmicb.2025.15533991553399Efficient utilization of Shuanghuanglian medicine residues by microbial transformation with flavonoid glycosides-hydrolyzing strainsXingwang Ma0Xingwang Ma1Ying Chen2Lili Li3Lili Li4Tianwei Wang5Kunling Teng6Junchang Su7Junchang Su8Lei Li9Liangliang Li10Heping Li11Wentao Diao12Guocan Chen13Jiwen Wang14Jin Zhong15Jin Zhong16State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaInstitute of Biology Co., Ltd., Henan Academy of Sciences, Zhengzhou, ChinaInstitute of Biology Co., Ltd., Henan Academy of Sciences, Zhengzhou, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou, ChinaInstitute of Biology Co., Ltd., Henan Academy of Sciences, Zhengzhou, ChinaInstitute of Biology Co., Ltd., Henan Academy of Sciences, Zhengzhou, ChinaInstitute of Biology Co., Ltd., Henan Academy of Sciences, Zhengzhou, ChinaState Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, ChinaCollege of Life Sciences, University of Chinese Academy of Sciences, Beijing, ChinaShuanghuanglian, a traditional Chinese medicine, is well-known for its bioactive compounds, such as flavonoids, which offer significant health benefits. However, the production of Shuanghuanlian generates substantial pharmaceutical residues, which are often discarded as waste, posing significant environmental and economic challenges. To date, research on repurposing these medicine residues has been limited. This study utilized beneficial microbes to efficiently extract and utilize the residual bioactive compounds. Notably, a newly isolated Lactiplantibacillus plantarum strain LLB exhibited remarkable efficiency in converting flavonoid glycosides (e.g., phillyrin and luteoloside) into their corresponding aglycones. When combined synergistically with Bacillus subtilis and Saccharomyces cerevisiae, strain LLB maintained robust flavonoid glycoside conversion while enhancing lactobacilli viability in the fermented medicine residues. As a feed additive for broiler chickens, the fermented residue not only boosted antioxidant (superoxide dismutase) and anti-inflammatory (IL-10) markers, but also preserved growth performance and meat quality. Furthermore, the fermented residue modulated the gut microbiome, increasing Rikenella while reducing Elusimicrobiota and Parabacteroides abundances. Our findings demonstrate that microbial transformation of Shuanghuanglian residues offers a sustainable strategy for waste valorization and a novel feed additive for enhancing animal health.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1553399/fullChinese medicine residueflavonoidLactiplantibacillus plantarumfeed additiveintestinal microbiota |
| spellingShingle | Xingwang Ma Xingwang Ma Ying Chen Lili Li Lili Li Tianwei Wang Kunling Teng Junchang Su Junchang Su Lei Li Liangliang Li Heping Li Wentao Diao Guocan Chen Jiwen Wang Jin Zhong Jin Zhong Efficient utilization of Shuanghuanglian medicine residues by microbial transformation with flavonoid glycosides-hydrolyzing strains Frontiers in Microbiology Chinese medicine residue flavonoid Lactiplantibacillus plantarum feed additive intestinal microbiota |
| title | Efficient utilization of Shuanghuanglian medicine residues by microbial transformation with flavonoid glycosides-hydrolyzing strains |
| title_full | Efficient utilization of Shuanghuanglian medicine residues by microbial transformation with flavonoid glycosides-hydrolyzing strains |
| title_fullStr | Efficient utilization of Shuanghuanglian medicine residues by microbial transformation with flavonoid glycosides-hydrolyzing strains |
| title_full_unstemmed | Efficient utilization of Shuanghuanglian medicine residues by microbial transformation with flavonoid glycosides-hydrolyzing strains |
| title_short | Efficient utilization of Shuanghuanglian medicine residues by microbial transformation with flavonoid glycosides-hydrolyzing strains |
| title_sort | efficient utilization of shuanghuanglian medicine residues by microbial transformation with flavonoid glycosides hydrolyzing strains |
| topic | Chinese medicine residue flavonoid Lactiplantibacillus plantarum feed additive intestinal microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1553399/full |
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