DEVELOPING A NEW ELECTROCHEMICAL ENZYME BIOSENSOR FOR HIGHLY SENSITIVE DETERMINATION OF HEAVY METAL IONS
Aim. This work was purposed to develop a new electrochemical enzyme biosensor for the highly sensitive determination of heavy metal ions. Methods. The electrochemical transducers consisted of two pairs of gold-interdigitated electrodes that connected to the impedimetric measuring device. Urease w...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2025-04-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | https://biotechnology.kiev.ua/images/BTA/2025/2_2025/Bakhmat2_2025%20.pdf |
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| Summary: | Aim. This work was purposed to develop a new electrochemical enzyme biosensor for the highly sensitive determination of heavy metal ions.
Methods. The electrochemical transducers consisted of two pairs of gold-interdigitated electrodes that connected to the impedimetric measuring device. Urease was immobilized with glutaraldehyde on one electrode pair, while a reference membrane with BSA was prepared similarly. Measurements were conducted in 5 mM PBS (pH 7.35) using differential mode to compensate for non-specific signal variations.
Results. The biosensor detected multiple HMIs with high sensitivity and showed high work reproducibility (RSD = 2.28%). EDTA treatment successfully restored enzyme activity, allowing various reuses. The sensor retained 85% activity after 18 months at 4 °C, confirming its long-term stability.
Conclusions. This biosensor provides a cost-effective, sensitive, and reusable platform for sensitive HMIs detection in wastewater, offering an alternative to conventional analytical techniques. |
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| ISSN: | 2410-7751 2410-776X |