Prenatal Dexamethasone Exposure Programs the Development of the Pancreas and the Secretion of Insulin in Rats
There is increasing epidemiological evidence indicating that many chronic diseases originate during early life, even before birth, through what are termed fetal programming effects. Prenatal glucocorticoid is frequently used clinically to accelerate the maturation of the lung, but its long-term effe...
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Elsevier
2017-04-01
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| Series: | Pediatrics and Neonatology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1875957216300675 |
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| author | Yu-Chieh Chen Ying-Hua Huang Jiunn-Ming Sheen You-Lin Tain Hong-Ren Yu Chih-Cheng Chen Miao-Meng Tiao Ho-Chang Kuo Li-Tung Huang |
| author_facet | Yu-Chieh Chen Ying-Hua Huang Jiunn-Ming Sheen You-Lin Tain Hong-Ren Yu Chih-Cheng Chen Miao-Meng Tiao Ho-Chang Kuo Li-Tung Huang |
| author_sort | Yu-Chieh Chen |
| collection | DOAJ |
| description | There is increasing epidemiological evidence indicating that many chronic diseases originate during early life, even before birth, through what are termed fetal programming effects. Prenatal glucocorticoid is frequently used clinically to accelerate the maturation of the lung, but its long-term effects remain unclear.
Methods: We gave pregnant Sprague–Dawley rats either intraperitoneal dexamethasone (0.1 mg/kg body weight) or vehicle at Gestational Days 14–20 and assessed the effects to pancreas at Postnatal Days 7 and 120.
Results: We found fewer pancreatic β cell fractions (0.31 ± 0.05 % vs. 0.49 ± 0.05 %, p = 0.013) and tissues (0.0017 ± 0.0002 % vs. 0.0025 ± 0.0002 %, p = 0.042) and decreased secretion of insulin in response to a glucose challenge at Postnatal Day 105 (1.00 ± 0.19 ng/mL vs. 1.57 ± 0.17 ng/mL at the 15-minute time-point, p = 0.046) in rats treated prenatally with dexamethasone. At Postnatal Day 7 in rats treated prenatally with dexamethasone, the expression of pancreatic duodenal homeobox gene-1 and V-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A was lower than that in the rats in the Vehicle group (0.22 ± 0.07 vs. 1.00 ± 0.41 fold, p = 0.01, 0.20 ± 0.12 vs. 1.00 ± 0.35 fold, p = 0.01) while the histone deacetylases activity (54.2 ± 3.7 ng/h/mL vs. 37.6 ± 3.5 ng/h/mL, p = 0.012) and 8-hydroxy-2-deoxyguanosine staining (1.34 ± 0.01 vs. 1.00 ± 0.02 fold, p < 0.01) were higher.
Conclusion: Prenatal dexamethasone exposure affects early postnatal gene expression related to pancreas development and may exert an effect on β-cell development at 120 postnatal days. |
| format | Article |
| id | doaj-art-ac49a9bb38df46f68ee3283f67f89e9a |
| institution | Kabale University |
| issn | 1875-9572 |
| language | English |
| publishDate | 2017-04-01 |
| publisher | Elsevier |
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| series | Pediatrics and Neonatology |
| spelling | doaj-art-ac49a9bb38df46f68ee3283f67f89e9a2025-08-20T03:24:30ZengElsevierPediatrics and Neonatology1875-95722017-04-0158213514410.1016/j.pedneo.2016.02.008Prenatal Dexamethasone Exposure Programs the Development of the Pancreas and the Secretion of Insulin in RatsYu-Chieh Chen0Ying-Hua Huang1Jiunn-Ming Sheen2You-Lin Tain3Hong-Ren Yu4Chih-Cheng Chen5Miao-Meng Tiao6Ho-Chang Kuo7Li-Tung Huang8Department of Pediatrics, Chang Gung Memorial Hospital—Kaohsiung Medical Center, Chang Gung University, College of Medicine, Kaohsiung, TaiwanDepartment of Pediatrics, Chang Gung Memorial Hospital—Kaohsiung Medical Center, Chang Gung University, College of Medicine, Kaohsiung, TaiwanDepartment of Pediatrics, Chang Gung Memorial Hospital—Kaohsiung Medical Center, Chang Gung University, College of Medicine, Kaohsiung, TaiwanDepartment of Pediatrics, Chang Gung Memorial Hospital—Kaohsiung Medical Center, Chang Gung University, College of Medicine, Kaohsiung, TaiwanDepartment of Pediatrics, Chang Gung Memorial Hospital—Kaohsiung Medical Center, Chang Gung University, College of Medicine, Kaohsiung, TaiwanDepartment of Pediatrics, Chang Gung Memorial Hospital—Kaohsiung Medical Center, Chang Gung University, College of Medicine, Kaohsiung, TaiwanDepartment of Pediatrics, Chang Gung Memorial Hospital—Kaohsiung Medical Center, Chang Gung University, College of Medicine, Kaohsiung, TaiwanDepartment of Pediatrics, Chang Gung Memorial Hospital—Kaohsiung Medical Center, Chang Gung University, College of Medicine, Kaohsiung, TaiwanDepartment of Pediatrics, Chang Gung Memorial Hospital—Kaohsiung Medical Center, Chang Gung University, College of Medicine, Kaohsiung, TaiwanThere is increasing epidemiological evidence indicating that many chronic diseases originate during early life, even before birth, through what are termed fetal programming effects. Prenatal glucocorticoid is frequently used clinically to accelerate the maturation of the lung, but its long-term effects remain unclear. Methods: We gave pregnant Sprague–Dawley rats either intraperitoneal dexamethasone (0.1 mg/kg body weight) or vehicle at Gestational Days 14–20 and assessed the effects to pancreas at Postnatal Days 7 and 120. Results: We found fewer pancreatic β cell fractions (0.31 ± 0.05 % vs. 0.49 ± 0.05 %, p = 0.013) and tissues (0.0017 ± 0.0002 % vs. 0.0025 ± 0.0002 %, p = 0.042) and decreased secretion of insulin in response to a glucose challenge at Postnatal Day 105 (1.00 ± 0.19 ng/mL vs. 1.57 ± 0.17 ng/mL at the 15-minute time-point, p = 0.046) in rats treated prenatally with dexamethasone. At Postnatal Day 7 in rats treated prenatally with dexamethasone, the expression of pancreatic duodenal homeobox gene-1 and V-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A was lower than that in the rats in the Vehicle group (0.22 ± 0.07 vs. 1.00 ± 0.41 fold, p = 0.01, 0.20 ± 0.12 vs. 1.00 ± 0.35 fold, p = 0.01) while the histone deacetylases activity (54.2 ± 3.7 ng/h/mL vs. 37.6 ± 3.5 ng/h/mL, p = 0.012) and 8-hydroxy-2-deoxyguanosine staining (1.34 ± 0.01 vs. 1.00 ± 0.02 fold, p < 0.01) were higher. Conclusion: Prenatal dexamethasone exposure affects early postnatal gene expression related to pancreas development and may exert an effect on β-cell development at 120 postnatal days.http://www.sciencedirect.com/science/article/pii/S1875957216300675diabetes mellituspancreasPDX-1prenatal dexamethasone exposureprogramming |
| spellingShingle | Yu-Chieh Chen Ying-Hua Huang Jiunn-Ming Sheen You-Lin Tain Hong-Ren Yu Chih-Cheng Chen Miao-Meng Tiao Ho-Chang Kuo Li-Tung Huang Prenatal Dexamethasone Exposure Programs the Development of the Pancreas and the Secretion of Insulin in Rats Pediatrics and Neonatology diabetes mellitus pancreas PDX-1 prenatal dexamethasone exposure programming |
| title | Prenatal Dexamethasone Exposure Programs the Development of the Pancreas and the Secretion of Insulin in Rats |
| title_full | Prenatal Dexamethasone Exposure Programs the Development of the Pancreas and the Secretion of Insulin in Rats |
| title_fullStr | Prenatal Dexamethasone Exposure Programs the Development of the Pancreas and the Secretion of Insulin in Rats |
| title_full_unstemmed | Prenatal Dexamethasone Exposure Programs the Development of the Pancreas and the Secretion of Insulin in Rats |
| title_short | Prenatal Dexamethasone Exposure Programs the Development of the Pancreas and the Secretion of Insulin in Rats |
| title_sort | prenatal dexamethasone exposure programs the development of the pancreas and the secretion of insulin in rats |
| topic | diabetes mellitus pancreas PDX-1 prenatal dexamethasone exposure programming |
| url | http://www.sciencedirect.com/science/article/pii/S1875957216300675 |
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