Development of a Method to Measure a Biomarker Panel Reflecting Dietary Exposure
Assessment of dietary intake remains a large challenge in nutrition studies. The application of food intake biomarkers is a promising approach to complement widely used self-reported intake assessments and to improve accuracy. The development of metabolomics has enabled the discovery of many potenti...
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MDPI AG
2024-04-01
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| Online Access: | https://www.mdpi.com/2504-3900/91/1/421 |
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| author | Stefania Noerman Marina Armeni Giuseppe Di Pede Raul Gonzalez-Domínguez Otto Savolainen Rikard Landberg |
| author_facet | Stefania Noerman Marina Armeni Giuseppe Di Pede Raul Gonzalez-Domínguez Otto Savolainen Rikard Landberg |
| author_sort | Stefania Noerman |
| collection | DOAJ |
| description | Assessment of dietary intake remains a large challenge in nutrition studies. The application of food intake biomarkers is a promising approach to complement widely used self-reported intake assessments and to improve accuracy. The development of metabolomics has enabled the discovery of many potential food intake biomarkers, but their applications are still limited. We aim to develop a semi-quantitative LC-MS/MS procedure to analyze a panel of plasma metabolites reflecting dietary exposure in a wide context. Our approach relies on a multi-analyte targeted LC-MS/MS method using a LC-QTRAP and commercially available reference compounds. A panel of 347 metabolites was selected, representing dietary intake (fruits and vegetables, coffee, tea, heat-treated food, wholegrain cereals, berries, cruciferous vegetables, and seafood) and key metabolites in the endogenous metabolism (fatty acids, amino acids, cholesterol metabolism, Krebs cycle, bile acids, and microbial metabolism) which are affected by specific diets, as well as lifestyle exposures, such as smoking and alcohol consumption. The application of this panel will help in assessing dietary exposures and their relationships to health outcomes. We will present the status of the work. |
| format | Article |
| id | doaj-art-ac42ec02829b43edb632b9cf8dcf3dae |
| institution | OA Journals |
| issn | 2504-3900 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proceedings |
| spelling | doaj-art-ac42ec02829b43edb632b9cf8dcf3dae2025-08-20T02:10:24ZengMDPI AGProceedings2504-39002024-04-0191142110.3390/proceedings2023091421Development of a Method to Measure a Biomarker Panel Reflecting Dietary ExposureStefania Noerman0Marina Armeni1Giuseppe Di Pede2Raul Gonzalez-Domínguez3Otto Savolainen4Rikard Landberg5Food and Nutrition Science Division, Department of Life Sciences, Chalmers University of Technology, 41258 Gothenburg, SwedenFood and Nutrition Science Division, Department of Life Sciences, Chalmers University of Technology, 41258 Gothenburg, SwedenDepartment of Food and Drugs, University of Parma, 43121 Parma, ItalyInstituto de Investigación e Innovación Biomédica de Cádiz (INiBICA), Hospital Universitario Puerta del Mar, Universidad de Cádiz, 11009 Cádiz, SpainChalmers Mass Spectrometry Infrastructure, Department of Life Sciences, Chalmers University of Technology, 41258 Gothenburg, SwedenFood and Nutrition Science Division, Department of Life Sciences, Chalmers University of Technology, 41258 Gothenburg, SwedenAssessment of dietary intake remains a large challenge in nutrition studies. The application of food intake biomarkers is a promising approach to complement widely used self-reported intake assessments and to improve accuracy. The development of metabolomics has enabled the discovery of many potential food intake biomarkers, but their applications are still limited. We aim to develop a semi-quantitative LC-MS/MS procedure to analyze a panel of plasma metabolites reflecting dietary exposure in a wide context. Our approach relies on a multi-analyte targeted LC-MS/MS method using a LC-QTRAP and commercially available reference compounds. A panel of 347 metabolites was selected, representing dietary intake (fruits and vegetables, coffee, tea, heat-treated food, wholegrain cereals, berries, cruciferous vegetables, and seafood) and key metabolites in the endogenous metabolism (fatty acids, amino acids, cholesterol metabolism, Krebs cycle, bile acids, and microbial metabolism) which are affected by specific diets, as well as lifestyle exposures, such as smoking and alcohol consumption. The application of this panel will help in assessing dietary exposures and their relationships to health outcomes. We will present the status of the work.https://www.mdpi.com/2504-3900/91/1/421dietary biomarkersmultiple reaction monitoringLC-MSplasma metabolitesendogenous metabolism |
| spellingShingle | Stefania Noerman Marina Armeni Giuseppe Di Pede Raul Gonzalez-Domínguez Otto Savolainen Rikard Landberg Development of a Method to Measure a Biomarker Panel Reflecting Dietary Exposure Proceedings dietary biomarkers multiple reaction monitoring LC-MS plasma metabolites endogenous metabolism |
| title | Development of a Method to Measure a Biomarker Panel Reflecting Dietary Exposure |
| title_full | Development of a Method to Measure a Biomarker Panel Reflecting Dietary Exposure |
| title_fullStr | Development of a Method to Measure a Biomarker Panel Reflecting Dietary Exposure |
| title_full_unstemmed | Development of a Method to Measure a Biomarker Panel Reflecting Dietary Exposure |
| title_short | Development of a Method to Measure a Biomarker Panel Reflecting Dietary Exposure |
| title_sort | development of a method to measure a biomarker panel reflecting dietary exposure |
| topic | dietary biomarkers multiple reaction monitoring LC-MS plasma metabolites endogenous metabolism |
| url | https://www.mdpi.com/2504-3900/91/1/421 |
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