Antibiotic Resistance, Virulence Genes, and Molecular Diversity of Clinical <i>Klebsiella pneumoniae</i> Isolates from Patients of District Hospital in Central Poland
In hospital environments, pathogenic bacteria spread easily and acquire virulence and antibiotic resistance genes. The aim of the study was an evaluation of the genetic diversity of 109 <i>K. pneumoniae</i> isolates recovered from patients of a district hospital in central Poland. The fr...
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2025-06-01
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| author | Barbara Kot Małgorzata Witeska Piotr Szweda Małgorzata Piechota Elżbieta Kondera Elżbieta Horoszewicz Izabela Balak Ahmer Bin Hafeez Alicja Synowiec |
| author_facet | Barbara Kot Małgorzata Witeska Piotr Szweda Małgorzata Piechota Elżbieta Kondera Elżbieta Horoszewicz Izabela Balak Ahmer Bin Hafeez Alicja Synowiec |
| author_sort | Barbara Kot |
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| description | In hospital environments, pathogenic bacteria spread easily and acquire virulence and antibiotic resistance genes. The aim of the study was an evaluation of the genetic diversity of 109 <i>K. pneumoniae</i> isolates recovered from patients of a district hospital in central Poland. The frequencies of genes coding for β-lactamases, efflux pumps, and virulence factors were determined. Genotyping of the isolates was performed with ERIC (Enterobacterial Repetitive Intergenic Consensus) and REP (Repetitive Element Sequence Based) PCR techniques, with 21 and 19 genotypes being identified, respectively. The <i>bla</i><sub>SHV-1</sub> (92.7%)<i>, bla</i><sub>CTX-M</sub> group 1 (83.5%), <i>bla</i><sub>TEM-1</sub> (28.4%), <i>bla</i><sub>NDM-1</sub> (16.5%)<i>, bla</i><sub>VEB-1</sub> (11.0%), <i>bla</i><sub>CTX-M</sub> group 9 (3.7%), <i>bla</i><sub>KPC</sub> (1.8%), <i>bla</i><sub>IMP</sub>, <i>bla</i><sub>OXA-48</sub>, <i>bla</i><sub>CTX-M</sub> group 2, <i>bla</i><sub>CTX-M</sub> groups 8, and 25/26 (0% each) and efflux pumps: <i>AcrAB</i> (100%)<i>, tolC</i> (93.6%)<i>,</i> and <i>mdtk</i> (60.5%), and virulence genes coding: urease subunit <i>ureA</i> (94.5%) endotoxins <i>wabG</i> (92.7%) and <i>uge</i> (64.2%), and siderophore <i>iucB</i> (3.7%) were detected. The <i>bla</i><sub>SHV-1</sub>, <i>bla</i><sub>CTX-M</sub> group 1, <i>mdtk</i>, <i>tolC</i>, <i>AcrAB</i> (16.5%); <i>bla</i><sub>SHV-1</sub>, <i>bla</i><sub>CTX-M</sub> group 1, <i>tolC</i>, <i>AcrAB</i> (15.6%), and <i>bla</i><sub>SHV-1</sub>, <i>bla</i><sub>CTX-M</sub> group 1, <i>bla</i><sub>NDM-1</sub>, <i>mdtk</i>, <i>tolC</i>, <i>AcrAB</i> (11.9%) were the most common resistance patterns. The distribution of resistance and virulence genes varied more between hospital wards than between different clinical materials. Hospital’s antibiotic-resistant and virulent <i>K. pneumoniae</i>, able to spread among humans, animals, and in the environment, pose a significant threat to public health. |
| format | Article |
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| institution | DOAJ |
| issn | 2076-0817 |
| language | English |
| publishDate | 2025-06-01 |
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| series | Pathogens |
| spelling | doaj-art-ac40b21c8e984871b3f0a5e83fff2c722025-08-20T02:47:20ZengMDPI AGPathogens2076-08172025-06-0114764810.3390/pathogens14070648Antibiotic Resistance, Virulence Genes, and Molecular Diversity of Clinical <i>Klebsiella pneumoniae</i> Isolates from Patients of District Hospital in Central PolandBarbara Kot0Małgorzata Witeska1Piotr Szweda2Małgorzata Piechota3Elżbieta Kondera4Elżbieta Horoszewicz5Izabela Balak6Ahmer Bin Hafeez7Alicja Synowiec8Institute of Biological Sciences, Faculty of Exact and Natural Sciences, University of Siedlce, 14 Bolesława Prusa Str., 08-110 Siedlce, PolandDepartment of Animal Environment Biology, Institute of Animal Science, Warsaw University of Life Sciences—SGGW, Ciszewskiego 8, 02-786 Warsaw, PolandDepartment of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdańsk University of Technology, 11/12 G. Narutowicza Str., 80-233 Gdańsk, PolandInstitute of Biological Sciences, Faculty of Exact and Natural Sciences, University of Siedlce, 14 Bolesława Prusa Str., 08-110 Siedlce, PolandInstitute of Biological Sciences, Faculty of Exact and Natural Sciences, University of Siedlce, 14 Bolesława Prusa Str., 08-110 Siedlce, PolandInstitute of Animal Science and Fisheries, University of Siedlce, 12 Bolesława Prusa Str., 08-110 Siedlce, PolandInstitute of Biological Sciences, Faculty of Exact and Natural Sciences, University of Siedlce, 14 Bolesława Prusa Str., 08-110 Siedlce, PolandDepartment of Pharmaceutical Technology and Biochemistry, Faculty of Chemistry, Gdańsk University of Technology, 11/12 G. Narutowicza Str., 80-233 Gdańsk, PolandDepartment of Food Biotechnology and Microbiology, Institute of Food Sciences, Warsaw University of Life Sciences—SGGW, Nowoursynowska 159c, 02-776 Warsaw, PolandIn hospital environments, pathogenic bacteria spread easily and acquire virulence and antibiotic resistance genes. The aim of the study was an evaluation of the genetic diversity of 109 <i>K. pneumoniae</i> isolates recovered from patients of a district hospital in central Poland. The frequencies of genes coding for β-lactamases, efflux pumps, and virulence factors were determined. Genotyping of the isolates was performed with ERIC (Enterobacterial Repetitive Intergenic Consensus) and REP (Repetitive Element Sequence Based) PCR techniques, with 21 and 19 genotypes being identified, respectively. The <i>bla</i><sub>SHV-1</sub> (92.7%)<i>, bla</i><sub>CTX-M</sub> group 1 (83.5%), <i>bla</i><sub>TEM-1</sub> (28.4%), <i>bla</i><sub>NDM-1</sub> (16.5%)<i>, bla</i><sub>VEB-1</sub> (11.0%), <i>bla</i><sub>CTX-M</sub> group 9 (3.7%), <i>bla</i><sub>KPC</sub> (1.8%), <i>bla</i><sub>IMP</sub>, <i>bla</i><sub>OXA-48</sub>, <i>bla</i><sub>CTX-M</sub> group 2, <i>bla</i><sub>CTX-M</sub> groups 8, and 25/26 (0% each) and efflux pumps: <i>AcrAB</i> (100%)<i>, tolC</i> (93.6%)<i>,</i> and <i>mdtk</i> (60.5%), and virulence genes coding: urease subunit <i>ureA</i> (94.5%) endotoxins <i>wabG</i> (92.7%) and <i>uge</i> (64.2%), and siderophore <i>iucB</i> (3.7%) were detected. The <i>bla</i><sub>SHV-1</sub>, <i>bla</i><sub>CTX-M</sub> group 1, <i>mdtk</i>, <i>tolC</i>, <i>AcrAB</i> (16.5%); <i>bla</i><sub>SHV-1</sub>, <i>bla</i><sub>CTX-M</sub> group 1, <i>tolC</i>, <i>AcrAB</i> (15.6%), and <i>bla</i><sub>SHV-1</sub>, <i>bla</i><sub>CTX-M</sub> group 1, <i>bla</i><sub>NDM-1</sub>, <i>mdtk</i>, <i>tolC</i>, <i>AcrAB</i> (11.9%) were the most common resistance patterns. The distribution of resistance and virulence genes varied more between hospital wards than between different clinical materials. Hospital’s antibiotic-resistant and virulent <i>K. pneumoniae</i>, able to spread among humans, animals, and in the environment, pose a significant threat to public health.https://www.mdpi.com/2076-0817/14/7/648β-lactamaseefflux pumpaerobactinureaseERIC-PCRREP-PCR |
| spellingShingle | Barbara Kot Małgorzata Witeska Piotr Szweda Małgorzata Piechota Elżbieta Kondera Elżbieta Horoszewicz Izabela Balak Ahmer Bin Hafeez Alicja Synowiec Antibiotic Resistance, Virulence Genes, and Molecular Diversity of Clinical <i>Klebsiella pneumoniae</i> Isolates from Patients of District Hospital in Central Poland Pathogens β-lactamase efflux pump aerobactin urease ERIC-PCR REP-PCR |
| title | Antibiotic Resistance, Virulence Genes, and Molecular Diversity of Clinical <i>Klebsiella pneumoniae</i> Isolates from Patients of District Hospital in Central Poland |
| title_full | Antibiotic Resistance, Virulence Genes, and Molecular Diversity of Clinical <i>Klebsiella pneumoniae</i> Isolates from Patients of District Hospital in Central Poland |
| title_fullStr | Antibiotic Resistance, Virulence Genes, and Molecular Diversity of Clinical <i>Klebsiella pneumoniae</i> Isolates from Patients of District Hospital in Central Poland |
| title_full_unstemmed | Antibiotic Resistance, Virulence Genes, and Molecular Diversity of Clinical <i>Klebsiella pneumoniae</i> Isolates from Patients of District Hospital in Central Poland |
| title_short | Antibiotic Resistance, Virulence Genes, and Molecular Diversity of Clinical <i>Klebsiella pneumoniae</i> Isolates from Patients of District Hospital in Central Poland |
| title_sort | antibiotic resistance virulence genes and molecular diversity of clinical i klebsiella pneumoniae i isolates from patients of district hospital in central poland |
| topic | β-lactamase efflux pump aerobactin urease ERIC-PCR REP-PCR |
| url | https://www.mdpi.com/2076-0817/14/7/648 |
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