The Development of ELISA-test System for Detection of Specific IgG to SARS-COV-2 Coronavirus by Immunoblotting (Line Blot)
Relevance. The problem of timely and effective diagnosis of COVID-19 remains one of the main problems facing healthcare. In this regard, the task of developing test systems for the etiological diagnosis of COVID-19 remains extremely relevant. Purpose To develop ELISA test system for detection of G s...
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| Format: | Article |
| Language: | Russian |
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Numikom LLC
2022-09-01
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| Series: | Эпидемиология и вакцинопрофилактика |
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| Online Access: | https://www.epidemvac.ru/jour/article/view/1617 |
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| _version_ | 1849250225529552896 |
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| author | S. G. Mardanly T. V. Popova |
| author_facet | S. G. Mardanly T. V. Popova |
| author_sort | S. G. Mardanly |
| collection | DOAJ |
| description | Relevance. The problem of timely and effective diagnosis of COVID-19 remains one of the main problems facing healthcare. In this regard, the task of developing test systems for the etiological diagnosis of COVID-19 remains extremely relevant. Purpose To develop ELISA test system for detection of G specific immunoglobulins to SARS-COV-2 coronavirus by immunoblotting (Line Blot). Methods. Elaboration of techniques for obtaining test components and preliminary assessment of its diagnostic effectivenessin blood serums from COVID-19 patients treated at N. I. Pirogov First Gradsky Hospital, Moscow, and serums from healthy human donors. Results. The study of 104 blood serum samples from COVID-19 patients and 100 blood serum samples from healthy human donors, pre-tested by ELISA IgG to SARS-CoV-2 using «Vitrotest SARS-CoV-2 IgG» test systems (Vitrotest, Ukraine) and «ELISA-SARS-CoV-2-AB-G» (CJSC EKOlab, Russia) showed high diagnostic efficiency of the new test system. Conclusion. The new test system after state registration of the medical device can be recommended as a confirmatory test for the etiological laboratory diagnosis of COVID-19. |
| format | Article |
| id | doaj-art-ac3e6b65f7b3451d9bf406e5d506cc55 |
| institution | Kabale University |
| issn | 2073-3046 2619-0494 |
| language | Russian |
| publishDate | 2022-09-01 |
| publisher | Numikom LLC |
| record_format | Article |
| series | Эпидемиология и вакцинопрофилактика |
| spelling | doaj-art-ac3e6b65f7b3451d9bf406e5d506cc552025-08-20T03:57:18ZrusNumikom LLCЭпидемиология и вакцинопрофилактика2073-30462619-04942022-09-0121410311210.31631/2073-3046-2022-21-4-103-112863The Development of ELISA-test System for Detection of Specific IgG to SARS-COV-2 Coronavirus by Immunoblotting (Line Blot)S. G. Mardanly0T. V. Popova1State University of Humanities and Technology; Closed Joint Stock Company EKOlab (CJSC EKOlab)Closed Joint Stock Company EKOlab (CJSC EKOlab)Relevance. The problem of timely and effective diagnosis of COVID-19 remains one of the main problems facing healthcare. In this regard, the task of developing test systems for the etiological diagnosis of COVID-19 remains extremely relevant. Purpose To develop ELISA test system for detection of G specific immunoglobulins to SARS-COV-2 coronavirus by immunoblotting (Line Blot). Methods. Elaboration of techniques for obtaining test components and preliminary assessment of its diagnostic effectivenessin blood serums from COVID-19 patients treated at N. I. Pirogov First Gradsky Hospital, Moscow, and serums from healthy human donors. Results. The study of 104 blood serum samples from COVID-19 patients and 100 blood serum samples from healthy human donors, pre-tested by ELISA IgG to SARS-CoV-2 using «Vitrotest SARS-CoV-2 IgG» test systems (Vitrotest, Ukraine) and «ELISA-SARS-CoV-2-AB-G» (CJSC EKOlab, Russia) showed high diagnostic efficiency of the new test system. Conclusion. The new test system after state registration of the medical device can be recommended as a confirmatory test for the etiological laboratory diagnosis of COVID-19.https://www.epidemvac.ru/jour/article/view/1617covid-19sars-cov-2laboratory diagnosticsdevelopmenttest systemline-blot |
| spellingShingle | S. G. Mardanly T. V. Popova The Development of ELISA-test System for Detection of Specific IgG to SARS-COV-2 Coronavirus by Immunoblotting (Line Blot) Эпидемиология и вакцинопрофилактика covid-19 sars-cov-2 laboratory diagnostics development test system line-blot |
| title | The Development of ELISA-test System for Detection of Specific IgG to SARS-COV-2 Coronavirus by Immunoblotting (Line Blot) |
| title_full | The Development of ELISA-test System for Detection of Specific IgG to SARS-COV-2 Coronavirus by Immunoblotting (Line Blot) |
| title_fullStr | The Development of ELISA-test System for Detection of Specific IgG to SARS-COV-2 Coronavirus by Immunoblotting (Line Blot) |
| title_full_unstemmed | The Development of ELISA-test System for Detection of Specific IgG to SARS-COV-2 Coronavirus by Immunoblotting (Line Blot) |
| title_short | The Development of ELISA-test System for Detection of Specific IgG to SARS-COV-2 Coronavirus by Immunoblotting (Line Blot) |
| title_sort | development of elisa test system for detection of specific igg to sars cov 2 coronavirus by immunoblotting line blot |
| topic | covid-19 sars-cov-2 laboratory diagnostics development test system line-blot |
| url | https://www.epidemvac.ru/jour/article/view/1617 |
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