Nageotte nodules in human dorsal root ganglia reveal neurodegeneration in diabetic peripheral neuropathy

Abstract Nageotte nodules, first described in 1922 by Jean Nageotte, are clusters of non-neuronal cells that form after sensory neuron death. Despite their historical recognition, little is known about their molecular identity nor their involvement in neuropathies that involve neuronal loss like dia...

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Main Authors: Stephanie I. Shiers, Khadijah Mazhar, Andi Wangzhou, Rainer Haberberger, Joseph B. Lesnak, Nwasinachi A. Ezeji, Ishwarya Sankaranarayanan, Diana Tavares-Ferreira, Anna Cervantes, Geoffrey Funk, Peter Horton, Erin Vines, Gregory Dussor, Theodore J. Price
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-59538-z
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author Stephanie I. Shiers
Khadijah Mazhar
Andi Wangzhou
Rainer Haberberger
Joseph B. Lesnak
Nwasinachi A. Ezeji
Ishwarya Sankaranarayanan
Diana Tavares-Ferreira
Anna Cervantes
Geoffrey Funk
Peter Horton
Erin Vines
Gregory Dussor
Theodore J. Price
author_facet Stephanie I. Shiers
Khadijah Mazhar
Andi Wangzhou
Rainer Haberberger
Joseph B. Lesnak
Nwasinachi A. Ezeji
Ishwarya Sankaranarayanan
Diana Tavares-Ferreira
Anna Cervantes
Geoffrey Funk
Peter Horton
Erin Vines
Gregory Dussor
Theodore J. Price
author_sort Stephanie I. Shiers
collection DOAJ
description Abstract Nageotte nodules, first described in 1922 by Jean Nageotte, are clusters of non-neuronal cells that form after sensory neuron death. Despite their historical recognition, little is known about their molecular identity nor their involvement in neuropathies that involve neuronal loss like diabetic peripheral neuropathy (DPN). In this study, we molecularly characterize Nageotte nodules in dorsal root ganglia recovered from organ donors with DPN. Here we show that Nageotte nodules are abundant in DPN sensory ganglia and account for 25% of all neurons. Peripherin-and Nav1.7-positive dystrophic axons invade Nageotte nodules, forming small neuroma-like structures. Using histology and spatial sequencing, we demonstrate that Nageotte nodules are mainly composed of satellite glia and non-myelinating Schwann cells that express SPP1 and are intertwined with sprouting sensory axons originating from neighboring neurons. Our findings suggest that Nageotte nodules are an integral feature of dorsal root ganglion neurodegeneration, providing potential therapeutic targets for sensory neuron protection and pain management in DPN.
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spelling doaj-art-ac3c3c5ff0a84f6fa72812ed1f80f32b2025-08-20T02:15:15ZengNature PortfolioNature Communications2041-17232025-05-0116111610.1038/s41467-025-59538-zNageotte nodules in human dorsal root ganglia reveal neurodegeneration in diabetic peripheral neuropathyStephanie I. Shiers0Khadijah Mazhar1Andi Wangzhou2Rainer Haberberger3Joseph B. Lesnak4Nwasinachi A. Ezeji5Ishwarya Sankaranarayanan6Diana Tavares-Ferreira7Anna Cervantes8Geoffrey Funk9Peter Horton10Erin Vines11Gregory Dussor12Theodore J. Price13Department of Neuroscience, Center for Advanced Pain Studies, The University of Texas at DallasDepartment of Neuroscience, Center for Advanced Pain Studies, The University of Texas at DallasDepartment of Neuroscience, Center for Advanced Pain Studies, The University of Texas at DallasAnatomy and Pathology, The University of AdelaideDepartment of Neuroscience, Center for Advanced Pain Studies, The University of Texas at DallasDepartment of Neuroscience, Center for Advanced Pain Studies, The University of Texas at DallasDepartment of Neuroscience, Center for Advanced Pain Studies, The University of Texas at DallasDepartment of Neuroscience, Center for Advanced Pain Studies, The University of Texas at DallasSouthwest Transplant AllianceSouthwest Transplant AllianceSouthwest Transplant AllianceSouthwest Transplant AllianceDepartment of Neuroscience, Center for Advanced Pain Studies, The University of Texas at DallasDepartment of Neuroscience, Center for Advanced Pain Studies, The University of Texas at DallasAbstract Nageotte nodules, first described in 1922 by Jean Nageotte, are clusters of non-neuronal cells that form after sensory neuron death. Despite their historical recognition, little is known about their molecular identity nor their involvement in neuropathies that involve neuronal loss like diabetic peripheral neuropathy (DPN). In this study, we molecularly characterize Nageotte nodules in dorsal root ganglia recovered from organ donors with DPN. Here we show that Nageotte nodules are abundant in DPN sensory ganglia and account for 25% of all neurons. Peripherin-and Nav1.7-positive dystrophic axons invade Nageotte nodules, forming small neuroma-like structures. Using histology and spatial sequencing, we demonstrate that Nageotte nodules are mainly composed of satellite glia and non-myelinating Schwann cells that express SPP1 and are intertwined with sprouting sensory axons originating from neighboring neurons. Our findings suggest that Nageotte nodules are an integral feature of dorsal root ganglion neurodegeneration, providing potential therapeutic targets for sensory neuron protection and pain management in DPN.https://doi.org/10.1038/s41467-025-59538-z
spellingShingle Stephanie I. Shiers
Khadijah Mazhar
Andi Wangzhou
Rainer Haberberger
Joseph B. Lesnak
Nwasinachi A. Ezeji
Ishwarya Sankaranarayanan
Diana Tavares-Ferreira
Anna Cervantes
Geoffrey Funk
Peter Horton
Erin Vines
Gregory Dussor
Theodore J. Price
Nageotte nodules in human dorsal root ganglia reveal neurodegeneration in diabetic peripheral neuropathy
Nature Communications
title Nageotte nodules in human dorsal root ganglia reveal neurodegeneration in diabetic peripheral neuropathy
title_full Nageotte nodules in human dorsal root ganglia reveal neurodegeneration in diabetic peripheral neuropathy
title_fullStr Nageotte nodules in human dorsal root ganglia reveal neurodegeneration in diabetic peripheral neuropathy
title_full_unstemmed Nageotte nodules in human dorsal root ganglia reveal neurodegeneration in diabetic peripheral neuropathy
title_short Nageotte nodules in human dorsal root ganglia reveal neurodegeneration in diabetic peripheral neuropathy
title_sort nageotte nodules in human dorsal root ganglia reveal neurodegeneration in diabetic peripheral neuropathy
url https://doi.org/10.1038/s41467-025-59538-z
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