Combining optical and electrical impedance techniques for quantitative measurement of confluence in MDCK-I cell cultures
A new method combining optical and electrical impedance measurements is described that enables submicroscopic cell movements to be monitored. The cells are grown on small gold electrodes that are transparent to light. This modified electrical cell-substrate impedance sensor (ECIS) allows simultaneou...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2004-04-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/04364RR01 |
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| _version_ | 1850152159410126848 |
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| author | Birgitte Freiesleben De Blasio Morten Laane Thomas Walmann Ivar Giaever |
| author_facet | Birgitte Freiesleben De Blasio Morten Laane Thomas Walmann Ivar Giaever |
| author_sort | Birgitte Freiesleben De Blasio |
| collection | DOAJ |
| description | A new method combining optical and electrical impedance measurements is described that enables submicroscopic cell movements to be monitored. The cells are grown on small gold electrodes that are transparent to light. This modified electrical cell-substrate impedance sensor (ECIS) allows simultaneous microscopic recording of both growth and motility, thus enabling cell confluence on the electrodes to be systematically correlated to the impedance in regular time intervals of seconds and for extended periods of time. Furthermore, the technique provides an independent measure of monolayer cell densities that we compare to calculated values from a theoretical model. We have followed the attachment and spreading behavior of epithelial Madin-Darby canine kidney strain I (MDCK-I) cell cultures on microelectrodes for up to 40 h. The studies reveal a high degree of correlation between the measured resistance at 4 kHz and the corresponding cell confluence in 4- to 6-h intervals with typical linear cross-correlation factors of r equaling approximately 0.9. In summary, the impedance measured with the ECIS technique provides a good quantitative measure of cell confluence. |
| format | Article |
| id | doaj-art-ac3af45806e04a6e93225cb7f410f730 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2004-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-ac3af45806e04a6e93225cb7f410f7302025-08-20T02:26:03ZengTaylor & Francis GroupBioTechniques0736-62051940-98182004-04-0136465066210.2144/04364RR01Combining optical and electrical impedance techniques for quantitative measurement of confluence in MDCK-I cell culturesBirgitte Freiesleben De Blasio0Morten Laane1Thomas Walmann2Ivar Giaever31Copenhagen University, Copenhagen, Denmark2University of Oslo, Oslo, Norway2University of Oslo, Oslo, Norway3Rensselaer Polytechnic Institute, Troy, NY, USAA new method combining optical and electrical impedance measurements is described that enables submicroscopic cell movements to be monitored. The cells are grown on small gold electrodes that are transparent to light. This modified electrical cell-substrate impedance sensor (ECIS) allows simultaneous microscopic recording of both growth and motility, thus enabling cell confluence on the electrodes to be systematically correlated to the impedance in regular time intervals of seconds and for extended periods of time. Furthermore, the technique provides an independent measure of monolayer cell densities that we compare to calculated values from a theoretical model. We have followed the attachment and spreading behavior of epithelial Madin-Darby canine kidney strain I (MDCK-I) cell cultures on microelectrodes for up to 40 h. The studies reveal a high degree of correlation between the measured resistance at 4 kHz and the corresponding cell confluence in 4- to 6-h intervals with typical linear cross-correlation factors of r equaling approximately 0.9. In summary, the impedance measured with the ECIS technique provides a good quantitative measure of cell confluence.https://www.future-science.com/doi/10.2144/04364RR01 |
| spellingShingle | Birgitte Freiesleben De Blasio Morten Laane Thomas Walmann Ivar Giaever Combining optical and electrical impedance techniques for quantitative measurement of confluence in MDCK-I cell cultures BioTechniques |
| title | Combining optical and electrical impedance techniques for quantitative measurement of confluence in MDCK-I cell cultures |
| title_full | Combining optical and electrical impedance techniques for quantitative measurement of confluence in MDCK-I cell cultures |
| title_fullStr | Combining optical and electrical impedance techniques for quantitative measurement of confluence in MDCK-I cell cultures |
| title_full_unstemmed | Combining optical and electrical impedance techniques for quantitative measurement of confluence in MDCK-I cell cultures |
| title_short | Combining optical and electrical impedance techniques for quantitative measurement of confluence in MDCK-I cell cultures |
| title_sort | combining optical and electrical impedance techniques for quantitative measurement of confluence in mdck i cell cultures |
| url | https://www.future-science.com/doi/10.2144/04364RR01 |
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