Maternal transmission of human papillomavirus in retinoblastoma: A possible route of transfer

Context: After establishing the presence of human papillomavirus (HPV) in retinoblastoma (RB), the probable role of the mother was investigated. Materials and Methods: A total of 21 sporadic RB cases and 15/21 corresponding mothers′ cervical brushings were collected. HPV testing was carried out usin...

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Main Authors: Anand Bhuvaneswari, V R Pallavi, R S Jayshree, Rekha V Kumar
Format: Article
Language:English
Published: Thieme Medical and Scientific Publishers Pvt. Ltd. 2012-01-01
Series:Indian Journal of Medical and Paediatric Oncology
Subjects:
Online Access:http://www.ijmpo.org/article.asp?issn=0971-5851;year=2012;volume=33;issue=4;spage=210;epage=215;aulast=Bhuvaneswari
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author Anand Bhuvaneswari
V R Pallavi
R S Jayshree
Rekha V Kumar
author_facet Anand Bhuvaneswari
V R Pallavi
R S Jayshree
Rekha V Kumar
author_sort Anand Bhuvaneswari
collection DOAJ
description Context: After establishing the presence of human papillomavirus (HPV) in retinoblastoma (RB), the probable role of the mother was investigated. Materials and Methods: A total of 21 sporadic RB cases and 15/21 corresponding mothers′ cervical brushings were collected. HPV testing was carried out using multiplex PCR (PGMY09/11 primers) followed by genotyping using line blot assay. Results: We found both high- (83%) and intermediate-risk (17%) HPV types in 12/21 (57%) RB samples and only high-risk (100%) types in 6/15 (40%) cervical brushing samples. The single genotype of HPV 16 was found in six cases and HPVs 82, 68 and 35 in one case each. Both HPVs 16 and 59 were found in two cases and HPV 16 and 73 in one case. Three samples of RB harboring HPV 16, HPVs 16 and 59, and HPVs 16 and 73 had HPV genotype 16 in the respective mothers′ cervical brushing samples. Conclusions: Maternal transfer of HPV in RB could be a possible route of transmission. However, a larger cohort and sampling of the mothers′ cervical brushings at various stages, i.e. before, during, and after pregnancy will give us insight to propound an alternate mechanism for the development of sporadic RB.
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spelling doaj-art-ac3283f4365947d1adc0329ef4fc7a322025-08-20T03:24:30ZengThieme Medical and Scientific Publishers Pvt. Ltd.Indian Journal of Medical and Paediatric Oncology0971-58512012-01-0133421021510.4103/0971-5851.107080Maternal transmission of human papillomavirus in retinoblastoma: A possible route of transferAnand BhuvaneswariV R PallaviR S JayshreeRekha V KumarContext: After establishing the presence of human papillomavirus (HPV) in retinoblastoma (RB), the probable role of the mother was investigated. Materials and Methods: A total of 21 sporadic RB cases and 15/21 corresponding mothers′ cervical brushings were collected. HPV testing was carried out using multiplex PCR (PGMY09/11 primers) followed by genotyping using line blot assay. Results: We found both high- (83%) and intermediate-risk (17%) HPV types in 12/21 (57%) RB samples and only high-risk (100%) types in 6/15 (40%) cervical brushing samples. The single genotype of HPV 16 was found in six cases and HPVs 82, 68 and 35 in one case each. Both HPVs 16 and 59 were found in two cases and HPV 16 and 73 in one case. Three samples of RB harboring HPV 16, HPVs 16 and 59, and HPVs 16 and 73 had HPV genotype 16 in the respective mothers′ cervical brushing samples. Conclusions: Maternal transfer of HPV in RB could be a possible route of transmission. However, a larger cohort and sampling of the mothers′ cervical brushings at various stages, i.e. before, during, and after pregnancy will give us insight to propound an alternate mechanism for the development of sporadic RB.http://www.ijmpo.org/article.asp?issn=0971-5851;year=2012;volume=33;issue=4;spage=210;epage=215;aulast=BhuvaneswariGenotypinghuman papillomavirusmaternal transmissionretinoblastoma
spellingShingle Anand Bhuvaneswari
V R Pallavi
R S Jayshree
Rekha V Kumar
Maternal transmission of human papillomavirus in retinoblastoma: A possible route of transfer
Indian Journal of Medical and Paediatric Oncology
Genotyping
human papillomavirus
maternal transmission
retinoblastoma
title Maternal transmission of human papillomavirus in retinoblastoma: A possible route of transfer
title_full Maternal transmission of human papillomavirus in retinoblastoma: A possible route of transfer
title_fullStr Maternal transmission of human papillomavirus in retinoblastoma: A possible route of transfer
title_full_unstemmed Maternal transmission of human papillomavirus in retinoblastoma: A possible route of transfer
title_short Maternal transmission of human papillomavirus in retinoblastoma: A possible route of transfer
title_sort maternal transmission of human papillomavirus in retinoblastoma a possible route of transfer
topic Genotyping
human papillomavirus
maternal transmission
retinoblastoma
url http://www.ijmpo.org/article.asp?issn=0971-5851;year=2012;volume=33;issue=4;spage=210;epage=215;aulast=Bhuvaneswari
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