Chromosome-level genome assembly of cultivated strawberry ‘Seolhyang’ (Fragaria × ananassa)
Abstract Cultivated strawberry (Fragaria × ananassa) belongs to the family Rosaceae and is an allo-octoploid species (2n = 8× = 56). Using PacBio Revio long reads of ‘Seolhyang’, we completed telomere-to-telomere phased genome assemblies with a size of 797 Mb with a contig N50 of 27.04 Mb. Benchmar...
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Nature Portfolio
2025-06-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-05191-6 |
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| author | Hyeondae Han Yoon Jeong Jang Koeun Han Han-Na Park Do-Sun Kim Seonghee Lee Youngjae Oh |
| author_facet | Hyeondae Han Yoon Jeong Jang Koeun Han Han-Na Park Do-Sun Kim Seonghee Lee Youngjae Oh |
| author_sort | Hyeondae Han |
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| description | Abstract Cultivated strawberry (Fragaria × ananassa) belongs to the family Rosaceae and is an allo-octoploid species (2n = 8× = 56). Using PacBio Revio long reads of ‘Seolhyang’, we completed telomere-to-telomere phased genome assemblies with a size of 797 Mb with a contig N50 of 27.04 Mb. Benchmarking of the universal single-copy orthologs (BUSCO) analysis detected 99.1% conserved genes in the assembly. In addition, the average long terminal repeat assembly index (LAI) was 17.28, with high genome continuity. In this study, we identified 50 of the possible 56 telomeres across 28 chromosomes. The ‘Seolhyang’ genome was annotated using RNA-Seq data representing various F. × ananassa tissues from the NCBI sequence read archive, which resulted in 129,184 genes. |
| format | Article |
| id | doaj-art-ac2e404b53e8457f895c1e880b057dee |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-ac2e404b53e8457f895c1e880b057dee2025-08-20T03:45:11ZengNature PortfolioScientific Data2052-44632025-06-0112111210.1038/s41597-025-05191-6Chromosome-level genome assembly of cultivated strawberry ‘Seolhyang’ (Fragaria × ananassa)Hyeondae Han0Yoon Jeong Jang1Koeun Han2Han-Na Park3Do-Sun Kim4Seonghee Lee5Youngjae Oh6Vegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development AdministrationVegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development AdministrationVegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development AdministrationStrawberry Research Institute, Chungcheongnam-do, ARESVegetable Research Division, National Institute of Horticultural and Herbal Science, Rural Development AdministrationDepartment of Horticultural Science, University of Florida, IFAS Gulf Coast Research and Education Center, 14625 CR 672Department of Horticultural Science, Chungbuk National UniversityAbstract Cultivated strawberry (Fragaria × ananassa) belongs to the family Rosaceae and is an allo-octoploid species (2n = 8× = 56). Using PacBio Revio long reads of ‘Seolhyang’, we completed telomere-to-telomere phased genome assemblies with a size of 797 Mb with a contig N50 of 27.04 Mb. Benchmarking of the universal single-copy orthologs (BUSCO) analysis detected 99.1% conserved genes in the assembly. In addition, the average long terminal repeat assembly index (LAI) was 17.28, with high genome continuity. In this study, we identified 50 of the possible 56 telomeres across 28 chromosomes. The ‘Seolhyang’ genome was annotated using RNA-Seq data representing various F. × ananassa tissues from the NCBI sequence read archive, which resulted in 129,184 genes.https://doi.org/10.1038/s41597-025-05191-6 |
| spellingShingle | Hyeondae Han Yoon Jeong Jang Koeun Han Han-Na Park Do-Sun Kim Seonghee Lee Youngjae Oh Chromosome-level genome assembly of cultivated strawberry ‘Seolhyang’ (Fragaria × ananassa) Scientific Data |
| title | Chromosome-level genome assembly of cultivated strawberry ‘Seolhyang’ (Fragaria × ananassa) |
| title_full | Chromosome-level genome assembly of cultivated strawberry ‘Seolhyang’ (Fragaria × ananassa) |
| title_fullStr | Chromosome-level genome assembly of cultivated strawberry ‘Seolhyang’ (Fragaria × ananassa) |
| title_full_unstemmed | Chromosome-level genome assembly of cultivated strawberry ‘Seolhyang’ (Fragaria × ananassa) |
| title_short | Chromosome-level genome assembly of cultivated strawberry ‘Seolhyang’ (Fragaria × ananassa) |
| title_sort | chromosome level genome assembly of cultivated strawberry seolhyang fragaria ananassa |
| url | https://doi.org/10.1038/s41597-025-05191-6 |
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