Screening of Bacillus thuringiensis and identification of insecticidal crystal protein gene against Bradysia difformis in mushroom cultivation
Bacillus thuringiensis (Bt) is exhibiting the high ability and wide insecticidal spectrum in the Sciaridae pest control. This experiment was conducted to screen out effective Bt strains and identify their insecticidal crystal protein (ICP) genotypes against Bradysia difformis in the mushroom cultiva...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2019-04-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2018.08.141 |
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| Summary: | Bacillus thuringiensis (Bt) is exhibiting the high ability and wide insecticidal spectrum in the Sciaridae pest control. This experiment was conducted to screen out effective Bt strains and identify their insecticidal crystal protein (ICP) genotypes against Bradysia difformis in the mushroom cultivation. The bacillus strains that produced crystals were separated from the soil of Zijin Mountain of Nanjing by sodium acetate-antibiotic method. The strains that showed insecticidal activity against larvae of B. difformis and inhibitory effect to the hyphae of mushroom were screened by using indoor toxicity measurement method and effect test on hyphae, separately. Then ICPs were identified based on polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP) analysis. Seven strains that showed high insecticidal activity (with the corrected mortality over 50%) against B. difformis and no influence on Pleurotus ostreatus were screened from 17 strains obtained from Nanjing. The 16S rDNA analysis results showed that the seven strains were homologous to B. thuringiensis with 99% identity. The PCR-RFLP system results revealed that the seven strains contain cry4/10, cry11, cyt1, and cyt2 genes. The Bt strains have potential for application to the biocontrol of B. difformis in mushroom cultivation. |
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| ISSN: | 1008-9209 2097-5155 |