Type 2 Innate Lymphoid Cell (Ilc2)-Deficient Mice Are Transcriptionally Constrained During <i>Nippostrongylus brasiliensis</i> Infection
Mouse models serve as a means of examining immune changes when genes of interest are knocked out (KO). One group of immune gene-producing cells that have been identified is type 2 innate lymphoid cells (Ilc2). These cells are involved in the production of Th2 equivalent immune responses and signal c...
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MDPI AG
2025-06-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/14/6/571 |
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| author | Damarius S. Fleming Fang Liu Joseph F. Urban Robert W. Li |
| author_facet | Damarius S. Fleming Fang Liu Joseph F. Urban Robert W. Li |
| author_sort | Damarius S. Fleming |
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| description | Mouse models serve as a means of examining immune changes when genes of interest are knocked out (KO). One group of immune gene-producing cells that have been identified is type 2 innate lymphoid cells (Ilc2). These cells are involved in the production of Th2 equivalent immune responses and signal cytokine production during the resolution of <i>Nippostrongylus brasiliensis</i> parasite infection in mice lungs. However, many questions about Ilc2 activity in the gut remain. To study this, retinoic acid receptor (RAR)-related orphan receptor alpha (<i>RORα</i>)-deficient mice were infected with adult <i>N. brasiliensis</i> and arranged into four treatment groups. Ten days post-infection (dpi), mouse ileum tissue was extracted for RNA-Seq. The <i>RORα</i>-deficient mice showed little change in gene expression at 10 dpi (N = 51) when compared to the WT mice at 10 dpi (N = 915), displaying dysregulation within the mouse gut. Based on the results, the gene expression in the gut of Ilc2-deficient mice denoted that the inability to craft Ilc2 cells left the mice unable to mount classical helminth immune responses involving humoral, mast cell, and antibody Th2-driven reactions. Overall, the results showed the importance of Ilc2 in the gut during <i>N. brasiliensis</i> infections and the effect that the lack of these cells had on immunity. |
| format | Article |
| id | doaj-art-ac25a7f75de041a5aa3b39c2d352197c |
| institution | OA Journals |
| issn | 2076-0817 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
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| series | Pathogens |
| spelling | doaj-art-ac25a7f75de041a5aa3b39c2d352197c2025-08-20T02:21:49ZengMDPI AGPathogens2076-08172025-06-0114657110.3390/pathogens14060571Type 2 Innate Lymphoid Cell (Ilc2)-Deficient Mice Are Transcriptionally Constrained During <i>Nippostrongylus brasiliensis</i> InfectionDamarius S. Fleming0Fang Liu1Joseph F. Urban2Robert W. Li3USDA-ARS, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705, USACollege of Public Health, Zhengzhou University, Zhengzhou 450001, ChinaUSDA-ARS, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705, USAUSDA-ARS, Beltsville Agricultural Research Center, Animal Parasitic Diseases Laboratory, Beltsville, MD 20705, USAMouse models serve as a means of examining immune changes when genes of interest are knocked out (KO). One group of immune gene-producing cells that have been identified is type 2 innate lymphoid cells (Ilc2). These cells are involved in the production of Th2 equivalent immune responses and signal cytokine production during the resolution of <i>Nippostrongylus brasiliensis</i> parasite infection in mice lungs. However, many questions about Ilc2 activity in the gut remain. To study this, retinoic acid receptor (RAR)-related orphan receptor alpha (<i>RORα</i>)-deficient mice were infected with adult <i>N. brasiliensis</i> and arranged into four treatment groups. Ten days post-infection (dpi), mouse ileum tissue was extracted for RNA-Seq. The <i>RORα</i>-deficient mice showed little change in gene expression at 10 dpi (N = 51) when compared to the WT mice at 10 dpi (N = 915), displaying dysregulation within the mouse gut. Based on the results, the gene expression in the gut of Ilc2-deficient mice denoted that the inability to craft Ilc2 cells left the mice unable to mount classical helminth immune responses involving humoral, mast cell, and antibody Th2-driven reactions. Overall, the results showed the importance of Ilc2 in the gut during <i>N. brasiliensis</i> infections and the effect that the lack of these cells had on immunity.https://www.mdpi.com/2076-0817/14/6/571gastrointestinalgene expressionIlc2immune responsehelminth |
| spellingShingle | Damarius S. Fleming Fang Liu Joseph F. Urban Robert W. Li Type 2 Innate Lymphoid Cell (Ilc2)-Deficient Mice Are Transcriptionally Constrained During <i>Nippostrongylus brasiliensis</i> Infection Pathogens gastrointestinal gene expression Ilc2 immune response helminth |
| title | Type 2 Innate Lymphoid Cell (Ilc2)-Deficient Mice Are Transcriptionally Constrained During <i>Nippostrongylus brasiliensis</i> Infection |
| title_full | Type 2 Innate Lymphoid Cell (Ilc2)-Deficient Mice Are Transcriptionally Constrained During <i>Nippostrongylus brasiliensis</i> Infection |
| title_fullStr | Type 2 Innate Lymphoid Cell (Ilc2)-Deficient Mice Are Transcriptionally Constrained During <i>Nippostrongylus brasiliensis</i> Infection |
| title_full_unstemmed | Type 2 Innate Lymphoid Cell (Ilc2)-Deficient Mice Are Transcriptionally Constrained During <i>Nippostrongylus brasiliensis</i> Infection |
| title_short | Type 2 Innate Lymphoid Cell (Ilc2)-Deficient Mice Are Transcriptionally Constrained During <i>Nippostrongylus brasiliensis</i> Infection |
| title_sort | type 2 innate lymphoid cell ilc2 deficient mice are transcriptionally constrained during i nippostrongylus brasiliensis i infection |
| topic | gastrointestinal gene expression Ilc2 immune response helminth |
| url | https://www.mdpi.com/2076-0817/14/6/571 |
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