Deciphering Biosynthesis Mechanism and Solution Properties of Cyclic Amylopectin
A novel cyclic amylopectin (CA) was synthesized from waxy corn starch (WCS) using <i>Bacillus stearothermophilus</i> branching enzyme (BstBE), providing insights into its biosynthesis mechanism and solution properties. During the first 4 h, BstBE partially cyclized WCS, producing 68.20%...
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MDPI AG
2024-10-01
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| author | Ruolan Li Yaning Shi Ming Miao |
| author_facet | Ruolan Li Yaning Shi Ming Miao |
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| description | A novel cyclic amylopectin (CA) was synthesized from waxy corn starch (WCS) using <i>Bacillus stearothermophilus</i> branching enzyme (BstBE), providing insights into its biosynthesis mechanism and solution properties. During the first 4 h, BstBE partially cyclized WCS, producing 68.20% CA with a significantly reduced molecular weight (M<sub>W</sub>), from 8.98 × 10⁶ to 3.19 × 10⁴ g/mol and a lower polymer dispersity index (PDI), decreasing from 1.97 to 1.12. This resulted in a uniform CA structure with shorter chain lengths, particularly increasing DP 3–13, especially DP 7–9. Over the subsequent 4–12 h, the PDI slightly increased to 1.18 as the CA content decreased to 50.48%, with an increase in small ring structures (DP 6–12) of CA, suggesting both ring-opening and ring-downsizing due to continued enzyme catalysis. These results propose a two-stage reaction model: initial cyclization followed bybranching and secondary cyclization. CA exhibited excellent solution properties, with BE-4 and BE-12 samples demonstrating high solubility (≥65 g/100 mL), low viscosity (<0.01 Pa·s), and over 90% light transmittance after 14 days at 4 °C, highlighting its broad application potential. |
| format | Article |
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| language | English |
| publishDate | 2024-10-01 |
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| series | Foods |
| spelling | doaj-art-ac1c4e6fd89f4c3695382a347fa47cdf2025-08-20T02:13:14ZengMDPI AGFoods2304-81582024-10-011321347410.3390/foods13213474Deciphering Biosynthesis Mechanism and Solution Properties of Cyclic AmylopectinRuolan Li0Yaning Shi1Ming Miao2State Key Laboratory of Food Science & Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, ChinaCollege of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, ChinaState Key Laboratory of Food Science & Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, ChinaA novel cyclic amylopectin (CA) was synthesized from waxy corn starch (WCS) using <i>Bacillus stearothermophilus</i> branching enzyme (BstBE), providing insights into its biosynthesis mechanism and solution properties. During the first 4 h, BstBE partially cyclized WCS, producing 68.20% CA with a significantly reduced molecular weight (M<sub>W</sub>), from 8.98 × 10⁶ to 3.19 × 10⁴ g/mol and a lower polymer dispersity index (PDI), decreasing from 1.97 to 1.12. This resulted in a uniform CA structure with shorter chain lengths, particularly increasing DP 3–13, especially DP 7–9. Over the subsequent 4–12 h, the PDI slightly increased to 1.18 as the CA content decreased to 50.48%, with an increase in small ring structures (DP 6–12) of CA, suggesting both ring-opening and ring-downsizing due to continued enzyme catalysis. These results propose a two-stage reaction model: initial cyclization followed bybranching and secondary cyclization. CA exhibited excellent solution properties, with BE-4 and BE-12 samples demonstrating high solubility (≥65 g/100 mL), low viscosity (<0.01 Pa·s), and over 90% light transmittance after 14 days at 4 °C, highlighting its broad application potential.https://www.mdpi.com/2304-8158/13/21/3474starchbranching enzymecyclic amylopectinbiosynthesissolution properties |
| spellingShingle | Ruolan Li Yaning Shi Ming Miao Deciphering Biosynthesis Mechanism and Solution Properties of Cyclic Amylopectin Foods starch branching enzyme cyclic amylopectin biosynthesis solution properties |
| title | Deciphering Biosynthesis Mechanism and Solution Properties of Cyclic Amylopectin |
| title_full | Deciphering Biosynthesis Mechanism and Solution Properties of Cyclic Amylopectin |
| title_fullStr | Deciphering Biosynthesis Mechanism and Solution Properties of Cyclic Amylopectin |
| title_full_unstemmed | Deciphering Biosynthesis Mechanism and Solution Properties of Cyclic Amylopectin |
| title_short | Deciphering Biosynthesis Mechanism and Solution Properties of Cyclic Amylopectin |
| title_sort | deciphering biosynthesis mechanism and solution properties of cyclic amylopectin |
| topic | starch branching enzyme cyclic amylopectin biosynthesis solution properties |
| url | https://www.mdpi.com/2304-8158/13/21/3474 |
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