A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological Analysis
A specific BaP hapten was synthesized and BaP antigens (immunogen, coating antigen) were prepared using diverse methods. Based on these works, a streptavidin-horseradish peroxidase-based enzyme-linked immunological analysis was developed and firstly used to detect BaP. Several physiochemical factors...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/8673476 |
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| author | Zhen Ma Huisheng Zhuang |
| author_facet | Zhen Ma Huisheng Zhuang |
| author_sort | Zhen Ma |
| collection | DOAJ |
| description | A specific BaP hapten was synthesized and BaP antigens (immunogen, coating antigen) were prepared using diverse methods. Based on these works, a streptavidin-horseradish peroxidase-based enzyme-linked immunological analysis was developed and firstly used to detect BaP. Several physiochemical factors that may influence the assay performance were optimized in the assay. Under optimal conditions, a detection limit of 0.0094 ng mL−1 was obtained and good linearity was achieved within a range of 0.03–35.40 ng mL−1. Satisfactory recovery of spiked samples (91.12–109.23%) was obtained and the coefficient of variation was acceptable. Finally, the detection results of BaP in environmental and food samples were consistent with those obtained using high-performance liquid chromatography. The proposed immunoassay is reliable and has great potential for detecting trace amounts of BaP. |
| format | Article |
| id | doaj-art-ac13ee3c4a19403481aef75295984003 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-ac13ee3c4a19403481aef752959840032025-02-03T05:59:47ZengWileyJournal of Chemistry2090-90632090-90712018-01-01201810.1155/2018/86734768673476A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological AnalysisZhen Ma0Huisheng Zhuang1School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, ChinaA specific BaP hapten was synthesized and BaP antigens (immunogen, coating antigen) were prepared using diverse methods. Based on these works, a streptavidin-horseradish peroxidase-based enzyme-linked immunological analysis was developed and firstly used to detect BaP. Several physiochemical factors that may influence the assay performance were optimized in the assay. Under optimal conditions, a detection limit of 0.0094 ng mL−1 was obtained and good linearity was achieved within a range of 0.03–35.40 ng mL−1. Satisfactory recovery of spiked samples (91.12–109.23%) was obtained and the coefficient of variation was acceptable. Finally, the detection results of BaP in environmental and food samples were consistent with those obtained using high-performance liquid chromatography. The proposed immunoassay is reliable and has great potential for detecting trace amounts of BaP.http://dx.doi.org/10.1155/2018/8673476 |
| spellingShingle | Zhen Ma Huisheng Zhuang A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological Analysis Journal of Chemistry |
| title | A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological Analysis |
| title_full | A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological Analysis |
| title_fullStr | A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological Analysis |
| title_full_unstemmed | A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological Analysis |
| title_short | A Chemical Synthesis of Benzo[a]pyrene Hapten and Its Application in a Streptavidin-Horseradish Peroxidase-Based Enzyme-Linked Immunological Analysis |
| title_sort | chemical synthesis of benzo a pyrene hapten and its application in a streptavidin horseradish peroxidase based enzyme linked immunological analysis |
| url | http://dx.doi.org/10.1155/2018/8673476 |
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