Histomorphometric Analysis of Osseointegrated Intraosseous Dental Implants Using Undecalcified Specimens: A Scoping Review

Bone histology and histomorphometry are reliable diagnostic tools for the assessment of the bone–implant interface, material safety and biocompatibility, and tissue response. They allow for the qualitative and quantitative analysis of undecalcified bone specimens. This scoping review aims to identif...

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Main Authors: Stefan Peev, Ralitsa Yotsova, Ivaylo Parushev
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Biomimetics
Subjects:
Online Access:https://www.mdpi.com/2313-7673/9/11/672
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author Stefan Peev
Ralitsa Yotsova
Ivaylo Parushev
author_facet Stefan Peev
Ralitsa Yotsova
Ivaylo Parushev
author_sort Stefan Peev
collection DOAJ
description Bone histology and histomorphometry are reliable diagnostic tools for the assessment of the bone–implant interface, material safety and biocompatibility, and tissue response. They allow for the qualitative and quantitative analysis of undecalcified bone specimens. This scoping review aims to identify the most common staining techniques, study models for in vivo experiments, and histomorphometric parameters used for quantitative bone evaluation of osseointegrated dental implants in the last decade. The Web of Science, PubMed, and Scopus databases were searched on 1 July 2024 for relevant articles in English, published in the last ten years, and the data were exported to an MS Excel spreadsheet. A total of 115 studies met the eligibility criteria and were included in the present review. The results indicate that the most common study models are dogs, rabbits, and pigs. Some of the most frequently used methods for the assessment of the bone–implant interface are the Toluidine blue, Stevenel’s blue with Van Gieson, and Levai–Laczko stainings. The results from this study demonstrate that the most commonly used histomorphometric parameters in implant dentistry are the bone-to-implant contact (BIC), bone area fraction occupancy (BAFO), bone area (BA), and bone density (BD). This review presents the recent trends in histomorphometric analysis of dental implants and identifies some research gaps that necessitate further research.
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spelling doaj-art-abfbfdfffb7b4f1489bb4930576d196f2025-08-20T01:53:40ZengMDPI AGBiomimetics2313-76732024-11-0191167210.3390/biomimetics9110672Histomorphometric Analysis of Osseointegrated Intraosseous Dental Implants Using Undecalcified Specimens: A Scoping ReviewStefan Peev0Ralitsa Yotsova1Ivaylo Parushev2Department of Periodontology and Dental Implantology, Faculty of Dental Medicine, Medical University of Varna, 9002 Varna, BulgariaDepartment of Oral Surgery, Faculty of Dental Medicine, Medical University of Varna, 9002 Varna, BulgariaDepartment of Clinical Medical Sciences, Faculty of Dental Medicine, Medical University of Varna, 9002 Varna, BulgariaBone histology and histomorphometry are reliable diagnostic tools for the assessment of the bone–implant interface, material safety and biocompatibility, and tissue response. They allow for the qualitative and quantitative analysis of undecalcified bone specimens. This scoping review aims to identify the most common staining techniques, study models for in vivo experiments, and histomorphometric parameters used for quantitative bone evaluation of osseointegrated dental implants in the last decade. The Web of Science, PubMed, and Scopus databases were searched on 1 July 2024 for relevant articles in English, published in the last ten years, and the data were exported to an MS Excel spreadsheet. A total of 115 studies met the eligibility criteria and were included in the present review. The results indicate that the most common study models are dogs, rabbits, and pigs. Some of the most frequently used methods for the assessment of the bone–implant interface are the Toluidine blue, Stevenel’s blue with Van Gieson, and Levai–Laczko stainings. The results from this study demonstrate that the most commonly used histomorphometric parameters in implant dentistry are the bone-to-implant contact (BIC), bone area fraction occupancy (BAFO), bone area (BA), and bone density (BD). This review presents the recent trends in histomorphometric analysis of dental implants and identifies some research gaps that necessitate further research.https://www.mdpi.com/2313-7673/9/11/672histologyhistomorphometryundecalcifiednon-decalcifieddental implantosseointegration
spellingShingle Stefan Peev
Ralitsa Yotsova
Ivaylo Parushev
Histomorphometric Analysis of Osseointegrated Intraosseous Dental Implants Using Undecalcified Specimens: A Scoping Review
Biomimetics
histology
histomorphometry
undecalcified
non-decalcified
dental implant
osseointegration
title Histomorphometric Analysis of Osseointegrated Intraosseous Dental Implants Using Undecalcified Specimens: A Scoping Review
title_full Histomorphometric Analysis of Osseointegrated Intraosseous Dental Implants Using Undecalcified Specimens: A Scoping Review
title_fullStr Histomorphometric Analysis of Osseointegrated Intraosseous Dental Implants Using Undecalcified Specimens: A Scoping Review
title_full_unstemmed Histomorphometric Analysis of Osseointegrated Intraosseous Dental Implants Using Undecalcified Specimens: A Scoping Review
title_short Histomorphometric Analysis of Osseointegrated Intraosseous Dental Implants Using Undecalcified Specimens: A Scoping Review
title_sort histomorphometric analysis of osseointegrated intraosseous dental implants using undecalcified specimens a scoping review
topic histology
histomorphometry
undecalcified
non-decalcified
dental implant
osseointegration
url https://www.mdpi.com/2313-7673/9/11/672
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AT ivayloparushev histomorphometricanalysisofosseointegratedintraosseousdentalimplantsusingundecalcifiedspecimensascopingreview