NHF-derived carbon dots: prevalidation approach in breast cancer treatment
Abstract Metastatic breast cancer dominates the female cancer-related mortality. Tumour-associated molecules represents a crucial for early disease detection and identification of novel therapeutic targets. Nanomaterial technologies provide promising novel approaches to disease diagnostics and thera...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-69670-z |
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| author | Crina Elena Tiron Gabriel Luta Mihail Butura Florin Zugun-Eloae Corneliu S. Stan Adina Coroaba Elena-Laura Ursu Gabriela Dumitrita Stanciu Adrian Tiron |
| author_facet | Crina Elena Tiron Gabriel Luta Mihail Butura Florin Zugun-Eloae Corneliu S. Stan Adina Coroaba Elena-Laura Ursu Gabriela Dumitrita Stanciu Adrian Tiron |
| author_sort | Crina Elena Tiron |
| collection | DOAJ |
| description | Abstract Metastatic breast cancer dominates the female cancer-related mortality. Tumour-associated molecules represents a crucial for early disease detection and identification of novel therapeutic targets. Nanomaterial technologies provide promising novel approaches to disease diagnostics and therapeutics. In the present study we extend the investigations of antitumoral properties of Carbon Dots prepared from N-hydroxyphthalimide (CD-NHF) precursor. We evaluate the effect of CD-NHF on tumour cell migration and invasion in vitro and their impact on tumour progression using an in vivo model. Furthermore, we investigate the molecular mechanisms involved in CD-NHF antitumour effects. In vivo mammary tumours were induced in Balb/c female mice by injecting 4T1 cells into the mammary fat pad. Conditional treatment with CD-NHF significantly impair both migration and invasion of metastatic breast cancer cells. The presence of CD-NHF within the 3D cell cultures strongly inhibited the malignant phenotype of MDA-MB-231, 4T1 and MCF-7 cells in 3D culture, resulting in culture colonies lacking invasive projections and reduction of mammospheres formation. Importantly, breast tumour growth and metastasis dissemination was significantly reduced upon CD-NHF treatments in a syngeneic mouse model and is associated with down-regulation of Ki67 and HSP90 expression. CD-NHF nanostructures provide exciting perspective for improving treatment outcome in breast cancer. |
| format | Article |
| id | doaj-art-abf2f4bf241c4ae490a603bd6ba5174c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2020-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-abf2f4bf241c4ae490a603bd6ba5174c2025-08-20T03:45:57ZengNature PortfolioScientific Reports2045-23222020-07-0110111010.1038/s41598-020-69670-zNHF-derived carbon dots: prevalidation approach in breast cancer treatmentCrina Elena Tiron0Gabriel Luta1Mihail Butura2Florin Zugun-Eloae3Corneliu S. Stan4Adina Coroaba5Elena-Laura Ursu6Gabriela Dumitrita Stanciu7Adrian Tiron8Regional Institute of Oncology, TRANSCEND CenterRegional Institute of Oncology, TRANSCEND CenterRegional Institute of Oncology, TRANSCEND CenterRegional Institute of Oncology, TRANSCEND CenterDepartment of Natural and Synthetic Polymers, “Gheorghe Asachi” Technical University of IasiDepartment of Chemistry, “Petru Poni” Institute of Macromolecular ChemistryDepartment of Chemistry, “Petru Poni” Institute of Macromolecular ChemistryCenter for Advanced Research and Development in Experimental Medicine (CEMEX), “Gr.T.Popa” University of Medicine and PharmacyRegional Institute of Oncology, TRANSCEND CenterAbstract Metastatic breast cancer dominates the female cancer-related mortality. Tumour-associated molecules represents a crucial for early disease detection and identification of novel therapeutic targets. Nanomaterial technologies provide promising novel approaches to disease diagnostics and therapeutics. In the present study we extend the investigations of antitumoral properties of Carbon Dots prepared from N-hydroxyphthalimide (CD-NHF) precursor. We evaluate the effect of CD-NHF on tumour cell migration and invasion in vitro and their impact on tumour progression using an in vivo model. Furthermore, we investigate the molecular mechanisms involved in CD-NHF antitumour effects. In vivo mammary tumours were induced in Balb/c female mice by injecting 4T1 cells into the mammary fat pad. Conditional treatment with CD-NHF significantly impair both migration and invasion of metastatic breast cancer cells. The presence of CD-NHF within the 3D cell cultures strongly inhibited the malignant phenotype of MDA-MB-231, 4T1 and MCF-7 cells in 3D culture, resulting in culture colonies lacking invasive projections and reduction of mammospheres formation. Importantly, breast tumour growth and metastasis dissemination was significantly reduced upon CD-NHF treatments in a syngeneic mouse model and is associated with down-regulation of Ki67 and HSP90 expression. CD-NHF nanostructures provide exciting perspective for improving treatment outcome in breast cancer.https://doi.org/10.1038/s41598-020-69670-z |
| spellingShingle | Crina Elena Tiron Gabriel Luta Mihail Butura Florin Zugun-Eloae Corneliu S. Stan Adina Coroaba Elena-Laura Ursu Gabriela Dumitrita Stanciu Adrian Tiron NHF-derived carbon dots: prevalidation approach in breast cancer treatment Scientific Reports |
| title | NHF-derived carbon dots: prevalidation approach in breast cancer treatment |
| title_full | NHF-derived carbon dots: prevalidation approach in breast cancer treatment |
| title_fullStr | NHF-derived carbon dots: prevalidation approach in breast cancer treatment |
| title_full_unstemmed | NHF-derived carbon dots: prevalidation approach in breast cancer treatment |
| title_short | NHF-derived carbon dots: prevalidation approach in breast cancer treatment |
| title_sort | nhf derived carbon dots prevalidation approach in breast cancer treatment |
| url | https://doi.org/10.1038/s41598-020-69670-z |
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