Transcriptomic profiling of pancreatic neuroendocrine tumors: dysregulation of WNT, MAPK, PI3K, neddylation pathways and potential non-invasive biomarkers.
The study aimed to identify altered signaling pathways and potential non-invasive biomarkers for pancreatic neuroendocrine tumors (PanNETs) through transcriptomic profiling of tumor tissues. The analysis encompassed samples from non-functional PanNETs (NF-PanNETs), insulinomas, and tumor-adjacent pa...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0325672 |
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| author | Helvijs Niedra Olesja Rogoza Rihards Saksis Raitis Peculis Anzela Halilova Aija Gerina Sofija Vilisova Natalja Senterjakova Aldis Pukitis Ignacio Ruz-Caracuel Julie Earl Georgina Kolnikova Peter Dubovan Miroslav Tomas Peter Makovicky Maria Urbanova Bozena Smolkova Eythimios Koniaris Ioanna Aggelioudaki Agapi Kataki Vita Rovite |
| author_facet | Helvijs Niedra Olesja Rogoza Rihards Saksis Raitis Peculis Anzela Halilova Aija Gerina Sofija Vilisova Natalja Senterjakova Aldis Pukitis Ignacio Ruz-Caracuel Julie Earl Georgina Kolnikova Peter Dubovan Miroslav Tomas Peter Makovicky Maria Urbanova Bozena Smolkova Eythimios Koniaris Ioanna Aggelioudaki Agapi Kataki Vita Rovite |
| author_sort | Helvijs Niedra |
| collection | DOAJ |
| description | The study aimed to identify altered signaling pathways and potential non-invasive biomarkers for pancreatic neuroendocrine tumors (PanNETs) through transcriptomic profiling of tumor tissues. The analysis encompassed samples from non-functional PanNETs (NF-PanNETs), insulinomas, and tumor-adjacent pancreatic tissues (TAPT). In the differential expression analysis comparing PanNETs and TAPTs, we identified 1,210 differentially expressed genes at a false discovery rate significance threshold of < 0.05 and with Log2FoldChange values of > 0.5 and <-0.5. Further pathway enrichment analysis revealed a multitude of overrepresented signaling pathways related to cell proliferation, survival, and tumorigenesis. Significant findings included the Beta-catenin-independent and TCF-dependent WNT signaling pathways, MAPK1/MAPK3 signaling, and terms associated with PI3K/AKT/mTOR signaling. Among the list of DEGs, we also identified 28 upregulated genes encoding cell surface proteins and 24 upregulated genes encoding cancer-associated secretome proteins. Since the proteins of these genes are found in the bloodstream, there is potential for further testing of these markers as biomarkers for liquid biopsy assays. Overall, these findings underscore the promise of transcriptomic landscape analysis in identifying PanNET-specific non-invasive biomarkers and uncovering potential therapeutic targets. |
| format | Article |
| id | doaj-art-abeba8a9a0ef479a82c5229255924d73 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-abeba8a9a0ef479a82c5229255924d732025-08-20T03:22:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01206e032567210.1371/journal.pone.0325672Transcriptomic profiling of pancreatic neuroendocrine tumors: dysregulation of WNT, MAPK, PI3K, neddylation pathways and potential non-invasive biomarkers.Helvijs NiedraOlesja RogozaRihards SaksisRaitis PeculisAnzela HalilovaAija GerinaSofija VilisovaNatalja SenterjakovaAldis PukitisIgnacio Ruz-CaracuelJulie EarlGeorgina KolnikovaPeter DubovanMiroslav TomasPeter MakovickyMaria UrbanovaBozena SmolkovaEythimios KoniarisIoanna AggelioudakiAgapi KatakiVita RoviteThe study aimed to identify altered signaling pathways and potential non-invasive biomarkers for pancreatic neuroendocrine tumors (PanNETs) through transcriptomic profiling of tumor tissues. The analysis encompassed samples from non-functional PanNETs (NF-PanNETs), insulinomas, and tumor-adjacent pancreatic tissues (TAPT). In the differential expression analysis comparing PanNETs and TAPTs, we identified 1,210 differentially expressed genes at a false discovery rate significance threshold of < 0.05 and with Log2FoldChange values of > 0.5 and <-0.5. Further pathway enrichment analysis revealed a multitude of overrepresented signaling pathways related to cell proliferation, survival, and tumorigenesis. Significant findings included the Beta-catenin-independent and TCF-dependent WNT signaling pathways, MAPK1/MAPK3 signaling, and terms associated with PI3K/AKT/mTOR signaling. Among the list of DEGs, we also identified 28 upregulated genes encoding cell surface proteins and 24 upregulated genes encoding cancer-associated secretome proteins. Since the proteins of these genes are found in the bloodstream, there is potential for further testing of these markers as biomarkers for liquid biopsy assays. Overall, these findings underscore the promise of transcriptomic landscape analysis in identifying PanNET-specific non-invasive biomarkers and uncovering potential therapeutic targets.https://doi.org/10.1371/journal.pone.0325672 |
| spellingShingle | Helvijs Niedra Olesja Rogoza Rihards Saksis Raitis Peculis Anzela Halilova Aija Gerina Sofija Vilisova Natalja Senterjakova Aldis Pukitis Ignacio Ruz-Caracuel Julie Earl Georgina Kolnikova Peter Dubovan Miroslav Tomas Peter Makovicky Maria Urbanova Bozena Smolkova Eythimios Koniaris Ioanna Aggelioudaki Agapi Kataki Vita Rovite Transcriptomic profiling of pancreatic neuroendocrine tumors: dysregulation of WNT, MAPK, PI3K, neddylation pathways and potential non-invasive biomarkers. PLoS ONE |
| title | Transcriptomic profiling of pancreatic neuroendocrine tumors: dysregulation of WNT, MAPK, PI3K, neddylation pathways and potential non-invasive biomarkers. |
| title_full | Transcriptomic profiling of pancreatic neuroendocrine tumors: dysregulation of WNT, MAPK, PI3K, neddylation pathways and potential non-invasive biomarkers. |
| title_fullStr | Transcriptomic profiling of pancreatic neuroendocrine tumors: dysregulation of WNT, MAPK, PI3K, neddylation pathways and potential non-invasive biomarkers. |
| title_full_unstemmed | Transcriptomic profiling of pancreatic neuroendocrine tumors: dysregulation of WNT, MAPK, PI3K, neddylation pathways and potential non-invasive biomarkers. |
| title_short | Transcriptomic profiling of pancreatic neuroendocrine tumors: dysregulation of WNT, MAPK, PI3K, neddylation pathways and potential non-invasive biomarkers. |
| title_sort | transcriptomic profiling of pancreatic neuroendocrine tumors dysregulation of wnt mapk pi3k neddylation pathways and potential non invasive biomarkers |
| url | https://doi.org/10.1371/journal.pone.0325672 |
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