Expression of interleukin 1-alpha, interleukin 6 and CD 10 in predicting recurrence of ameloblastoma

Abstract Purpose Ameloblastoma (AM) is a clinically significant odontogenic tumour known for its local invasive and high post-treatment recurrence risk. Despite advancements in surgical techniques, predicting recurrence remains challenging. The role of immunomarkers in predicting recurrence of amelo...

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Main Authors: Nurhazwani Mohd Danil, Bogahawatte Samarakoon Mudiyanselage Samadarani Siriwardena, Yet Ching Goh, Wanninayake Mudiyanselage Tilakaratne
Format: Article
Language:English
Published: Springer 2025-07-01
Series:Discover Oncology
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Online Access:https://doi.org/10.1007/s12672-025-03235-2
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author Nurhazwani Mohd Danil
Bogahawatte Samarakoon Mudiyanselage Samadarani Siriwardena
Yet Ching Goh
Wanninayake Mudiyanselage Tilakaratne
author_facet Nurhazwani Mohd Danil
Bogahawatte Samarakoon Mudiyanselage Samadarani Siriwardena
Yet Ching Goh
Wanninayake Mudiyanselage Tilakaratne
author_sort Nurhazwani Mohd Danil
collection DOAJ
description Abstract Purpose Ameloblastoma (AM) is a clinically significant odontogenic tumour known for its local invasive and high post-treatment recurrence risk. Despite advancements in surgical techniques, predicting recurrence remains challenging. The role of immunomarkers in predicting recurrence of ameloblastoma remains non-conclusive. The aim of this study is to investigate the predictability of recurrence using selected immune markers. Methods 42 AM samples comprising 16 non-recurrent AM (AMNR), 13 primary tumour of recurrent AM (PAMR), and 13 recurrent AM from the same patient as PAMR (RAM) were immunohistochemically examined for the expression of interleukin 1-alpha (IL-1α), interleukin 6 (IL-6) and CD 10. Immunoreactive scoring (IRS) was used to assess the expression levels. The expression levels were further analyzed for the correlation with demographic and clinicopathological parameters of interest. Results The three markers were heterogeneously expressed in the ameloblastoma samples (IL-1α = 97.6%; IL-6 = 97.6% and CD 10 = 90.5%). Major findings were the upregulation of IL-6 (RAM > PAMR > AMNR) in RAM, while IL-1α and CD 10 scored higher in AMNR (AMNR > PAMR > RAM). Further correlation with clinicopathological parameters showed significant association between IL-1α expression with histopathological variants in AMNR (p = 0.03) and PAMR (p = 0.002). IL-6 expression was significantly correlated with tumour locality in AMNR (p = 0.01), and CD 10 showed significant correlation with tumour locality in PAMR (p = 0.02) and subsites of tumour locality in PAMR (p = 0.005) and RAM (p = 0.002). Conclusion The upregulation of IL-6 in recurrent ameloblastoma may predict its potential for recurrence. However, this interpretation should be considered tentative due to the inherent limitations of the study. Immunoexpression of IL-1α and CD 10 requires further investigation to elucidate their roles in tumourigenesis and invasiveness of ameloblastoma.
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spelling doaj-art-abe9742614aa41a8b5a4809c0022c2172025-08-20T04:02:54ZengSpringerDiscover Oncology2730-60112025-07-0116111610.1007/s12672-025-03235-2Expression of interleukin 1-alpha, interleukin 6 and CD 10 in predicting recurrence of ameloblastomaNurhazwani Mohd Danil0Bogahawatte Samarakoon Mudiyanselage Samadarani Siriwardena1Yet Ching Goh2Wanninayake Mudiyanselage Tilakaratne3Department of Oral and Maxillofacial Clinical Sciences, Faculty of Dentistry, Universiti MalayaDepartment of Oral Pathology, Faculty of Dental Sciences, University of PeradeniyaDepartment of Oral and Maxillofacial Clinical Sciences, Faculty of Dentistry, Universiti MalayaDepartment of Oral and Maxillofacial Clinical Sciences, Faculty of Dentistry, Universiti MalayaAbstract Purpose Ameloblastoma (AM) is a clinically significant odontogenic tumour known for its local invasive and high post-treatment recurrence risk. Despite advancements in surgical techniques, predicting recurrence remains challenging. The role of immunomarkers in predicting recurrence of ameloblastoma remains non-conclusive. The aim of this study is to investigate the predictability of recurrence using selected immune markers. Methods 42 AM samples comprising 16 non-recurrent AM (AMNR), 13 primary tumour of recurrent AM (PAMR), and 13 recurrent AM from the same patient as PAMR (RAM) were immunohistochemically examined for the expression of interleukin 1-alpha (IL-1α), interleukin 6 (IL-6) and CD 10. Immunoreactive scoring (IRS) was used to assess the expression levels. The expression levels were further analyzed for the correlation with demographic and clinicopathological parameters of interest. Results The three markers were heterogeneously expressed in the ameloblastoma samples (IL-1α = 97.6%; IL-6 = 97.6% and CD 10 = 90.5%). Major findings were the upregulation of IL-6 (RAM > PAMR > AMNR) in RAM, while IL-1α and CD 10 scored higher in AMNR (AMNR > PAMR > RAM). Further correlation with clinicopathological parameters showed significant association between IL-1α expression with histopathological variants in AMNR (p = 0.03) and PAMR (p = 0.002). IL-6 expression was significantly correlated with tumour locality in AMNR (p = 0.01), and CD 10 showed significant correlation with tumour locality in PAMR (p = 0.02) and subsites of tumour locality in PAMR (p = 0.005) and RAM (p = 0.002). Conclusion The upregulation of IL-6 in recurrent ameloblastoma may predict its potential for recurrence. However, this interpretation should be considered tentative due to the inherent limitations of the study. Immunoexpression of IL-1α and CD 10 requires further investigation to elucidate their roles in tumourigenesis and invasiveness of ameloblastoma.https://doi.org/10.1007/s12672-025-03235-2AmeloblastomaIL-1αIL-6CD10Recurrence
spellingShingle Nurhazwani Mohd Danil
Bogahawatte Samarakoon Mudiyanselage Samadarani Siriwardena
Yet Ching Goh
Wanninayake Mudiyanselage Tilakaratne
Expression of interleukin 1-alpha, interleukin 6 and CD 10 in predicting recurrence of ameloblastoma
Discover Oncology
Ameloblastoma
IL-1α
IL-6
CD10
Recurrence
title Expression of interleukin 1-alpha, interleukin 6 and CD 10 in predicting recurrence of ameloblastoma
title_full Expression of interleukin 1-alpha, interleukin 6 and CD 10 in predicting recurrence of ameloblastoma
title_fullStr Expression of interleukin 1-alpha, interleukin 6 and CD 10 in predicting recurrence of ameloblastoma
title_full_unstemmed Expression of interleukin 1-alpha, interleukin 6 and CD 10 in predicting recurrence of ameloblastoma
title_short Expression of interleukin 1-alpha, interleukin 6 and CD 10 in predicting recurrence of ameloblastoma
title_sort expression of interleukin 1 alpha interleukin 6 and cd 10 in predicting recurrence of ameloblastoma
topic Ameloblastoma
IL-1α
IL-6
CD10
Recurrence
url https://doi.org/10.1007/s12672-025-03235-2
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