Dietary isobutyric acid supplementation improves intestinal mucosal barrier function and meat quality by regulating cecal microbiota and serum metabolites in weaned piglets
This study aimed to provide evidence for the effects of isobutyric acid on the intestinal mucosal barrier and gut microbiota in weaned piglets. In this study, 30 piglets were divided into two groups: one group was fed a standard diet (CON group), and the other group was fed a diet supplemented with...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1565216/full |
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| author | Binjie Wang Junjie Hou Yundong Cao Haibo Wei Kangle Sun Xiang Ji Xiaoran Chu Yu Zhang Sen Jiang Linlin Shi Ke Liu Zhen Song Zhen Song Fengyun Wen Fengyun Wen |
| author_facet | Binjie Wang Junjie Hou Yundong Cao Haibo Wei Kangle Sun Xiang Ji Xiaoran Chu Yu Zhang Sen Jiang Linlin Shi Ke Liu Zhen Song Zhen Song Fengyun Wen Fengyun Wen |
| author_sort | Binjie Wang |
| collection | DOAJ |
| description | This study aimed to provide evidence for the effects of isobutyric acid on the intestinal mucosal barrier and gut microbiota in weaned piglets. In this study, 30 piglets were divided into two groups: one group was fed a standard diet (CON group), and the other group was fed a diet supplemented with 0.5% isobutyric acid (IB group) for 21 days. The results showed that isobutyric acid significantly increased (p < 0.05) serum immunity and antioxidant capacity in weaned piglets. In small intestine of piglets, the ratio of villus height to crypt depth was significantly increased (p < 0.05). Administration of isobutyric acid also increased (p < 0.05) the expression of genes related to intestinal mucosal barrier function. Cecal microbiota analysis revealed that isobutyric acid significantly increased (p < 0.05) the abundance of the Eubacterium coprostanoligenes group. Untargeted serum metabolomics analysis indicated that the top three categories of metabolites were lipids and lipid-like molecules, organic acids and derivatives, and organic heterocyclic compounds. Additionally, in longissimus thoracis muscle, isobutyric acid significantly increased (p < 0 0.05) intramuscular fat and triglyceride content compared with the CON group. Overall, isobutyric acid can improve small intestinal mucosal barrier function, and may influence the fat deposition through the regulation of serum metabolites in weaned piglets. |
| format | Article |
| id | doaj-art-abcdce9e2a834ac487d58bf7678131af |
| institution | OA Journals |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-abcdce9e2a834ac487d58bf7678131af2025-08-20T02:13:28ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-05-011210.3389/fvets.2025.15652161565216Dietary isobutyric acid supplementation improves intestinal mucosal barrier function and meat quality by regulating cecal microbiota and serum metabolites in weaned pigletsBinjie Wang0Junjie Hou1Yundong Cao2Haibo Wei3Kangle Sun4Xiang Ji5Xiaoran Chu6Yu Zhang7Sen Jiang8Linlin Shi9Ke Liu10Zhen Song11Zhen Song12Fengyun Wen13Fengyun Wen14College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, ChinaAnqiu County Animal Husbandry Development Center, Weifang, Shandong, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, ChinaThe Kay Laboratory of High Quality Livestock and Poultry Germplasm Resources and Genetic Breeding of Luoyang, Luoyang, Henan, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang, Henan, ChinaThe Kay Laboratory of High Quality Livestock and Poultry Germplasm Resources and Genetic Breeding of Luoyang, Luoyang, Henan, ChinaThis study aimed to provide evidence for the effects of isobutyric acid on the intestinal mucosal barrier and gut microbiota in weaned piglets. In this study, 30 piglets were divided into two groups: one group was fed a standard diet (CON group), and the other group was fed a diet supplemented with 0.5% isobutyric acid (IB group) for 21 days. The results showed that isobutyric acid significantly increased (p < 0.05) serum immunity and antioxidant capacity in weaned piglets. In small intestine of piglets, the ratio of villus height to crypt depth was significantly increased (p < 0.05). Administration of isobutyric acid also increased (p < 0.05) the expression of genes related to intestinal mucosal barrier function. Cecal microbiota analysis revealed that isobutyric acid significantly increased (p < 0.05) the abundance of the Eubacterium coprostanoligenes group. Untargeted serum metabolomics analysis indicated that the top three categories of metabolites were lipids and lipid-like molecules, organic acids and derivatives, and organic heterocyclic compounds. Additionally, in longissimus thoracis muscle, isobutyric acid significantly increased (p < 0 0.05) intramuscular fat and triglyceride content compared with the CON group. Overall, isobutyric acid can improve small intestinal mucosal barrier function, and may influence the fat deposition through the regulation of serum metabolites in weaned piglets.https://www.frontiersin.org/articles/10.3389/fvets.2025.1565216/fullisobutyric acidintestinal mucosal barrier functiongut microbiotaintramuscular fatserum metabolomeweaned piglets |
| spellingShingle | Binjie Wang Junjie Hou Yundong Cao Haibo Wei Kangle Sun Xiang Ji Xiaoran Chu Yu Zhang Sen Jiang Linlin Shi Ke Liu Zhen Song Zhen Song Fengyun Wen Fengyun Wen Dietary isobutyric acid supplementation improves intestinal mucosal barrier function and meat quality by regulating cecal microbiota and serum metabolites in weaned piglets Frontiers in Veterinary Science isobutyric acid intestinal mucosal barrier function gut microbiota intramuscular fat serum metabolome weaned piglets |
| title | Dietary isobutyric acid supplementation improves intestinal mucosal barrier function and meat quality by regulating cecal microbiota and serum metabolites in weaned piglets |
| title_full | Dietary isobutyric acid supplementation improves intestinal mucosal barrier function and meat quality by regulating cecal microbiota and serum metabolites in weaned piglets |
| title_fullStr | Dietary isobutyric acid supplementation improves intestinal mucosal barrier function and meat quality by regulating cecal microbiota and serum metabolites in weaned piglets |
| title_full_unstemmed | Dietary isobutyric acid supplementation improves intestinal mucosal barrier function and meat quality by regulating cecal microbiota and serum metabolites in weaned piglets |
| title_short | Dietary isobutyric acid supplementation improves intestinal mucosal barrier function and meat quality by regulating cecal microbiota and serum metabolites in weaned piglets |
| title_sort | dietary isobutyric acid supplementation improves intestinal mucosal barrier function and meat quality by regulating cecal microbiota and serum metabolites in weaned piglets |
| topic | isobutyric acid intestinal mucosal barrier function gut microbiota intramuscular fat serum metabolome weaned piglets |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1565216/full |
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