pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity
Experimental autoimmune encephalomyelitis (EAE) is a demyelinating pathology of the central nervous system (CNS) used as a model to study multiple sclerosis immunopathology. EAE has also been extensively employed to evaluate potentially therapeutic schemes. Considering the presence of an immune resp...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2017/6257958 |
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| author | Sofia Fernanda Gonçalves Zorzella-Pezavento Fernanda Chiuso-Minicucci Thais Graziela Donegá França Larissa Lumi Watanabe Ishikawa Larissa Camargo da Rosa Priscila Maria Colavite Bianca Balbino Camila Marques Maura Rosane Valerio Ikoma Ana Paula Masson Célio Lopes Silva Alexandrina Sartori |
| author_facet | Sofia Fernanda Gonçalves Zorzella-Pezavento Fernanda Chiuso-Minicucci Thais Graziela Donegá França Larissa Lumi Watanabe Ishikawa Larissa Camargo da Rosa Priscila Maria Colavite Bianca Balbino Camila Marques Maura Rosane Valerio Ikoma Ana Paula Masson Célio Lopes Silva Alexandrina Sartori |
| author_sort | Sofia Fernanda Gonçalves Zorzella-Pezavento |
| collection | DOAJ |
| description | Experimental autoimmune encephalomyelitis (EAE) is a demyelinating pathology of the central nervous system (CNS) used as a model to study multiple sclerosis immunopathology. EAE has also been extensively employed to evaluate potentially therapeutic schemes. Considering the presence of an immune response directed to heat shock proteins (hsps) in autoimmune diseases and the immunoregulatory potential of these molecules, we evaluated the effect of a previous immunization with a genetic vaccine containing the mycobacterial hsp65 gene on EAE development. C57BL/6 mice were immunized with 4 pVAXhsp65 doses and 14 days later were submitted to EAE induction by immunization with myelin oligodendrocyte glycoprotein (MOG35–55) emulsified in Complete Freund’s Adjuvant. Vaccinated mice presented significant lower clinical scores and lost less body weight. MOG35–55 immunization also determined less inflammation in lumbar spinal cord but did not change CD4+CD25+Foxp3+ T cells frequency in spleen and CNS. Infiltrating cells from the CNS stimulated with rhsp65 produced significantly higher levels of IL-10. These results suggest that the ability of pVAXhsp65 vaccination to control EAE development is associated with IL-10 induction. |
| format | Article |
| id | doaj-art-abccc886b45b473290fdb4e811f4296b |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-abccc886b45b473290fdb4e811f4296b2025-02-03T01:11:00ZengWileyJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/62579586257958pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis SeveritySofia Fernanda Gonçalves Zorzella-Pezavento0Fernanda Chiuso-Minicucci1Thais Graziela Donegá França2Larissa Lumi Watanabe Ishikawa3Larissa Camargo da Rosa4Priscila Maria Colavite5Bianca Balbino6Camila Marques7Maura Rosane Valerio Ikoma8Ana Paula Masson9Célio Lopes Silva10Alexandrina Sartori11Department of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, BrazilDepartment of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, BrazilDepartment of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, BrazilDepartment of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, BrazilDepartment of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, BrazilDepartment of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, BrazilDepartment of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, BrazilFlow Cytometry Laboratory, Amaral Carvalho Foundation, Jaú, SP, BrazilFlow Cytometry Laboratory, Amaral Carvalho Foundation, Jaú, SP, BrazilDepartment of Biochemistry and Immunology, University of São Paulo (USP), Ribeirão Preto, SP, BrazilDepartment of Biochemistry and Immunology, University of São Paulo (USP), Ribeirão Preto, SP, BrazilDepartment of Microbiology and Immunology, Institute of Biosciences of Botucatu, Universidade Estadual Paulista (UNESP), Botucatu, SP, BrazilExperimental autoimmune encephalomyelitis (EAE) is a demyelinating pathology of the central nervous system (CNS) used as a model to study multiple sclerosis immunopathology. EAE has also been extensively employed to evaluate potentially therapeutic schemes. Considering the presence of an immune response directed to heat shock proteins (hsps) in autoimmune diseases and the immunoregulatory potential of these molecules, we evaluated the effect of a previous immunization with a genetic vaccine containing the mycobacterial hsp65 gene on EAE development. C57BL/6 mice were immunized with 4 pVAXhsp65 doses and 14 days later were submitted to EAE induction by immunization with myelin oligodendrocyte glycoprotein (MOG35–55) emulsified in Complete Freund’s Adjuvant. Vaccinated mice presented significant lower clinical scores and lost less body weight. MOG35–55 immunization also determined less inflammation in lumbar spinal cord but did not change CD4+CD25+Foxp3+ T cells frequency in spleen and CNS. Infiltrating cells from the CNS stimulated with rhsp65 produced significantly higher levels of IL-10. These results suggest that the ability of pVAXhsp65 vaccination to control EAE development is associated with IL-10 induction.http://dx.doi.org/10.1155/2017/6257958 |
| spellingShingle | Sofia Fernanda Gonçalves Zorzella-Pezavento Fernanda Chiuso-Minicucci Thais Graziela Donegá França Larissa Lumi Watanabe Ishikawa Larissa Camargo da Rosa Priscila Maria Colavite Bianca Balbino Camila Marques Maura Rosane Valerio Ikoma Ana Paula Masson Célio Lopes Silva Alexandrina Sartori pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity Journal of Immunology Research |
| title | pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity |
| title_full | pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity |
| title_fullStr | pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity |
| title_full_unstemmed | pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity |
| title_short | pVAXhsp65 Vaccination Primes for High IL-10 Production and Decreases Experimental Encephalomyelitis Severity |
| title_sort | pvaxhsp65 vaccination primes for high il 10 production and decreases experimental encephalomyelitis severity |
| url | http://dx.doi.org/10.1155/2017/6257958 |
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