A study on the mnemotropic and antidepressant-like effects of the low-molecular-weight mimetic of nerve growth factor, dipeptide GK-2, in experimental ischemic stroke
Relevance. The nerve growth factor (NGF), possessing neuroprotective and neuroregenerative properties, holds promise for the development of medications for the treatment of post-stroke conditions. At the Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies, a...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
LLC “Publisher OKI”
2024-06-01
|
| Series: | Фармакокинетика и Фармакодинамика |
| Subjects: | |
| Online Access: | https://www.pharmacokinetica.ru/jour/article/view/406 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849344888653479936 |
|---|---|
| author | P. Yu. Povarnina N. M. Sazonova D. M. Nikiforov T. A. Gudasheva V. L. Dorofeev |
| author_facet | P. Yu. Povarnina N. M. Sazonova D. M. Nikiforov T. A. Gudasheva V. L. Dorofeev |
| author_sort | P. Yu. Povarnina |
| collection | DOAJ |
| description | Relevance. The nerve growth factor (NGF), possessing neuroprotective and neuroregenerative properties, holds promise for the development of medications for the treatment of post-stroke conditions. At the Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies, a dimeric dipeptide mimic of NGF with the laboratory code GK-2 was designed and synthesized. Under conditions of experimental cerebral ischemia, it significantly reduced the volume of brain infarction and stimulated neuro- and synaptogenesis.Objective. The aim of this study was to investigate the effects of GK-2 on experimental post-stroke dementia and depression.Methods. Ischemic stroke was induced by occlusion of the middle cerebral artery (MCAO) in Wistar rats. GK-2 was administered intraperitoneally at a dose of 0.5 mg/kg for 21 days post MCAO. Short-term and long-term memory of the animals were assessed 30–40 days post MCAO using the novel object recognition test. Depressive-like state was evaluated through the forced swimming test and sucrose preference test.Results. In animals subjected to MCAO, both short-term and long-term memory exhibited a statistically significant decline of 80 %, along with a depressive-like state characterized by a 50 % increase in total immobility time and a 30 % reduction in sucrose preference, when compared to the sham-lesioned group. Dipeptide GK-2 completely averted the onset of these impairments.Conclusion. The dipeptide mimic of NGF, GK-2, mitigates the development of cognitive and psychomotional impairments in the setting of experimental stroke. |
| format | Article |
| id | doaj-art-abc97dadc5dd418eafc6078384111850 |
| institution | Kabale University |
| issn | 2587-7836 2686-8830 |
| language | Russian |
| publishDate | 2024-06-01 |
| publisher | LLC “Publisher OKI” |
| record_format | Article |
| series | Фармакокинетика и Фармакодинамика |
| spelling | doaj-art-abc97dadc5dd418eafc60783841118502025-08-20T03:42:33ZrusLLC “Publisher OKI”Фармакокинетика и Фармакодинамика2587-78362686-88302024-06-0101142210.37489/2587-7836-2024-1-14-22364A study on the mnemotropic and antidepressant-like effects of the low-molecular-weight mimetic of nerve growth factor, dipeptide GK-2, in experimental ischemic strokeP. Yu. Povarnina0N. M. Sazonova1D. M. Nikiforov2T. A. Gudasheva3V. L. Dorofeev4FSBSI «Federal research center for innovator and emerging biomedical and pharmaceutical technologies»FSBSI «Federal research center for innovator and emerging biomedical and pharmaceutical technologies»FSBSI «Federal research center for innovator and emerging biomedical and pharmaceutical technologies»FSBSI «Federal research center for innovator and emerging biomedical and pharmaceutical technologies»FSBSI «Federal research center for innovator and emerging biomedical and pharmaceutical technologies»Relevance. The nerve growth factor (NGF), possessing neuroprotective and neuroregenerative properties, holds promise for the development of medications for the treatment of post-stroke conditions. At the Federal Research Center for Innovator and Emerging Biomedical and Pharmaceutical Technologies, a dimeric dipeptide mimic of NGF with the laboratory code GK-2 was designed and synthesized. Under conditions of experimental cerebral ischemia, it significantly reduced the volume of brain infarction and stimulated neuro- and synaptogenesis.Objective. The aim of this study was to investigate the effects of GK-2 on experimental post-stroke dementia and depression.Methods. Ischemic stroke was induced by occlusion of the middle cerebral artery (MCAO) in Wistar rats. GK-2 was administered intraperitoneally at a dose of 0.5 mg/kg for 21 days post MCAO. Short-term and long-term memory of the animals were assessed 30–40 days post MCAO using the novel object recognition test. Depressive-like state was evaluated through the forced swimming test and sucrose preference test.Results. In animals subjected to MCAO, both short-term and long-term memory exhibited a statistically significant decline of 80 %, along with a depressive-like state characterized by a 50 % increase in total immobility time and a 30 % reduction in sucrose preference, when compared to the sham-lesioned group. Dipeptide GK-2 completely averted the onset of these impairments.Conclusion. The dipeptide mimic of NGF, GK-2, mitigates the development of cognitive and psychomotional impairments in the setting of experimental stroke.https://www.pharmacokinetica.ru/jour/article/view/406ngfdipeptide mimeticpost-stroke depressionpost-stroke dementia |
| spellingShingle | P. Yu. Povarnina N. M. Sazonova D. M. Nikiforov T. A. Gudasheva V. L. Dorofeev A study on the mnemotropic and antidepressant-like effects of the low-molecular-weight mimetic of nerve growth factor, dipeptide GK-2, in experimental ischemic stroke Фармакокинетика и Фармакодинамика ngf dipeptide mimetic post-stroke depression post-stroke dementia |
| title | A study on the mnemotropic and antidepressant-like effects of the low-molecular-weight mimetic of nerve growth factor, dipeptide GK-2, in experimental ischemic stroke |
| title_full | A study on the mnemotropic and antidepressant-like effects of the low-molecular-weight mimetic of nerve growth factor, dipeptide GK-2, in experimental ischemic stroke |
| title_fullStr | A study on the mnemotropic and antidepressant-like effects of the low-molecular-weight mimetic of nerve growth factor, dipeptide GK-2, in experimental ischemic stroke |
| title_full_unstemmed | A study on the mnemotropic and antidepressant-like effects of the low-molecular-weight mimetic of nerve growth factor, dipeptide GK-2, in experimental ischemic stroke |
| title_short | A study on the mnemotropic and antidepressant-like effects of the low-molecular-weight mimetic of nerve growth factor, dipeptide GK-2, in experimental ischemic stroke |
| title_sort | study on the mnemotropic and antidepressant like effects of the low molecular weight mimetic of nerve growth factor dipeptide gk 2 in experimental ischemic stroke |
| topic | ngf dipeptide mimetic post-stroke depression post-stroke dementia |
| url | https://www.pharmacokinetica.ru/jour/article/view/406 |
| work_keys_str_mv | AT pyupovarnina astudyonthemnemotropicandantidepressantlikeeffectsofthelowmolecularweightmimeticofnervegrowthfactordipeptidegk2inexperimentalischemicstroke AT nmsazonova astudyonthemnemotropicandantidepressantlikeeffectsofthelowmolecularweightmimeticofnervegrowthfactordipeptidegk2inexperimentalischemicstroke AT dmnikiforov astudyonthemnemotropicandantidepressantlikeeffectsofthelowmolecularweightmimeticofnervegrowthfactordipeptidegk2inexperimentalischemicstroke AT tagudasheva astudyonthemnemotropicandantidepressantlikeeffectsofthelowmolecularweightmimeticofnervegrowthfactordipeptidegk2inexperimentalischemicstroke AT vldorofeev astudyonthemnemotropicandantidepressantlikeeffectsofthelowmolecularweightmimeticofnervegrowthfactordipeptidegk2inexperimentalischemicstroke AT pyupovarnina studyonthemnemotropicandantidepressantlikeeffectsofthelowmolecularweightmimeticofnervegrowthfactordipeptidegk2inexperimentalischemicstroke AT nmsazonova studyonthemnemotropicandantidepressantlikeeffectsofthelowmolecularweightmimeticofnervegrowthfactordipeptidegk2inexperimentalischemicstroke AT dmnikiforov studyonthemnemotropicandantidepressantlikeeffectsofthelowmolecularweightmimeticofnervegrowthfactordipeptidegk2inexperimentalischemicstroke AT tagudasheva studyonthemnemotropicandantidepressantlikeeffectsofthelowmolecularweightmimeticofnervegrowthfactordipeptidegk2inexperimentalischemicstroke AT vldorofeev studyonthemnemotropicandantidepressantlikeeffectsofthelowmolecularweightmimeticofnervegrowthfactordipeptidegk2inexperimentalischemicstroke |