Functional analysis of GmCPDs and investigation of their roles in flowering.
The onset of floral development is a pivotal switch in the life of soybean. Brassinosteroids (BRs), a group of steroidal phytohormones with essential roles in plant growth and development, are associated with flowering induction. Genes involved in BR biosynthesis have been studied to a great extent...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0118476&type=printable |
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| author | Miao Wang Xin Xu Xinxin Zhang Shi Sun Cunxiang Wu Wensheng Hou Qingyu Wang Tianfu Han |
| author_facet | Miao Wang Xin Xu Xinxin Zhang Shi Sun Cunxiang Wu Wensheng Hou Qingyu Wang Tianfu Han |
| author_sort | Miao Wang |
| collection | DOAJ |
| description | The onset of floral development is a pivotal switch in the life of soybean. Brassinosteroids (BRs), a group of steroidal phytohormones with essential roles in plant growth and development, are associated with flowering induction. Genes involved in BR biosynthesis have been studied to a great extent in Arabidopsis, but the study of these genes has been limited in soybean. In this study, four CPD homologs (GmCPDs) catalyzing BR synthesis were isolated from soybean. Transcripts were mainly confined to cotyledons and leaves and were down-regulated in response to exogenous BR. Bioinformatic analysis showed strong sequence and structure similarity between GmCPDs and AtCPD as well as CPDs of other species. Overexpression of GmCPDs in an Arabidopsis BR-deficient mutant rescued the phenotype by restoring the biosynthesis pathway, revealing the functional roles of each GmCPDs in. Except for the rescue of root development, leaf expansion and plant type architecture, GmCPDs in expression also complemented the late flowering phenotype of Arabidopsis mutants deficient in CPD. Further evidence in soybean plants is that the expression levels of GmCPDs in are under photoperiod control in Zigongdongdou, a photoperiod-sensitive variety, and show a sudden peak upon floral meristem initiation. Together with increased GmCPDs in expression in the leaves and cotyledons of photoperiod-insensitive early-maturity soybean, it is clear that GmCPDs in contribute to flowering development and are essential in the early stages of flowering regulation. |
| format | Article |
| id | doaj-art-abc8d6e5693746c3854ca9beaaee96e9 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-abc8d6e5693746c3854ca9beaaee96e92025-08-20T03:10:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e011847610.1371/journal.pone.0118476Functional analysis of GmCPDs and investigation of their roles in flowering.Miao WangXin XuXinxin ZhangShi SunCunxiang WuWensheng HouQingyu WangTianfu HanThe onset of floral development is a pivotal switch in the life of soybean. Brassinosteroids (BRs), a group of steroidal phytohormones with essential roles in plant growth and development, are associated with flowering induction. Genes involved in BR biosynthesis have been studied to a great extent in Arabidopsis, but the study of these genes has been limited in soybean. In this study, four CPD homologs (GmCPDs) catalyzing BR synthesis were isolated from soybean. Transcripts were mainly confined to cotyledons and leaves and were down-regulated in response to exogenous BR. Bioinformatic analysis showed strong sequence and structure similarity between GmCPDs and AtCPD as well as CPDs of other species. Overexpression of GmCPDs in an Arabidopsis BR-deficient mutant rescued the phenotype by restoring the biosynthesis pathway, revealing the functional roles of each GmCPDs in. Except for the rescue of root development, leaf expansion and plant type architecture, GmCPDs in expression also complemented the late flowering phenotype of Arabidopsis mutants deficient in CPD. Further evidence in soybean plants is that the expression levels of GmCPDs in are under photoperiod control in Zigongdongdou, a photoperiod-sensitive variety, and show a sudden peak upon floral meristem initiation. Together with increased GmCPDs in expression in the leaves and cotyledons of photoperiod-insensitive early-maturity soybean, it is clear that GmCPDs in contribute to flowering development and are essential in the early stages of flowering regulation.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0118476&type=printable |
| spellingShingle | Miao Wang Xin Xu Xinxin Zhang Shi Sun Cunxiang Wu Wensheng Hou Qingyu Wang Tianfu Han Functional analysis of GmCPDs and investigation of their roles in flowering. PLoS ONE |
| title | Functional analysis of GmCPDs and investigation of their roles in flowering. |
| title_full | Functional analysis of GmCPDs and investigation of their roles in flowering. |
| title_fullStr | Functional analysis of GmCPDs and investigation of their roles in flowering. |
| title_full_unstemmed | Functional analysis of GmCPDs and investigation of their roles in flowering. |
| title_short | Functional analysis of GmCPDs and investigation of their roles in flowering. |
| title_sort | functional analysis of gmcpds and investigation of their roles in flowering |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0118476&type=printable |
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