Chaihuang Qingyi Huoxue granule ameliorates severe acute pancreatitis by modulating gut microbiota and repairing the intestinal mucosal barrier
BackgroundDuring severe acute pancreatitis (SAP), damage to the intestinal mucosal barrier and translocation of intestinal pathogenic bacteria are key mechanisms that accelerate the disease progression of SAP. Chaihuang Qingyi Huoxue Granule (CH) is a herbal formula used in the clinical treatment of...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1514201/full |
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| author | Xiaobin Zhang Xusen Zeng Wen Guo Xin Zhou Xin Zhou Yi Zhang Mingyun Tang Juan Fu Yuqing Deng Xin Liang Long Zhao Zhi Li Zhi Li Zhi Li Tiangang Wang Li Li Guohui Xiao |
| author_facet | Xiaobin Zhang Xusen Zeng Wen Guo Xin Zhou Xin Zhou Yi Zhang Mingyun Tang Juan Fu Yuqing Deng Xin Liang Long Zhao Zhi Li Zhi Li Zhi Li Tiangang Wang Li Li Guohui Xiao |
| author_sort | Xiaobin Zhang |
| collection | DOAJ |
| description | BackgroundDuring severe acute pancreatitis (SAP), damage to the intestinal mucosal barrier and translocation of intestinal pathogenic bacteria are key mechanisms that accelerate the disease progression of SAP. Chaihuang Qingyi Huoxue Granule (CH) is a herbal formula used in the clinical treatment of SAP. This study aims to investigate the role of CH in regulating gut microbiota and intestinal mucosal barrier in SAP rats.MethodsSodium taurocholate (3.5%) was retrogradely perfused into the biliopancreatic duct to establish the model of SAP in rats. CH (4.4 g/kg) was administered by gavage. Serum amylase, lipase, and endotoxin levels were measured. Hematoxylin-eosin (HE) staining was used to observe morphological changes in the pancreas and colon. The expression of zona occludens-1 (ZO-1) and occludin in the colon was examined by immunohistochemistry (IHC) and western blot. 16S rDNA gene sequencing was used to analyze the gut microbiota of the rats. The content of short-chain fatty acids (SCFAs) in the intestinal contents of the rats was determined by gas chromatography-mass spectrometry (GC-MS).ResultsCH reduced serum amylase, lipase, and endotoxin levels in SAP rats, alleviated pathological damage in the pancreas and colon, and restored the expression of ZO-1 and occludin. Moreover, CH alleviated gut microbiota dysbiosis in SAP rats, with restored gut microbiota diversity and structure. At the phylum level, the relative abundance of Firmicutes and Bacteroidetes increased, while that of Proteobacteria decreased. At the genus level, the abundance of Ruminococcus 1, Parabacteroides, Prevotellaceae UCG-001, Lachnospiraceae NK4A136 group, and Lactobacillus increased, while that of Escherichia-Shigella, Enterococcus, and Enterobacter decreased. In addition, CH increased the levels of SCFAs in the intestinal contents of SAP rats.ConclusionCH ameliorates SAP by maintaining the homeostasis and diversity of the gut microbiota, increasing the levels of SCFAs, and repairing the intestinal mucosal barrier. |
| format | Article |
| id | doaj-art-abc13b2aee7a4028831c7c02eb7706a6 |
| institution | DOAJ |
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| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-abc13b2aee7a4028831c7c02eb7706a62025-08-20T03:01:03ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-02-011510.3389/fcimb.2025.15142011514201Chaihuang Qingyi Huoxue granule ameliorates severe acute pancreatitis by modulating gut microbiota and repairing the intestinal mucosal barrierXiaobin Zhang0Xusen Zeng1Wen Guo2Xin Zhou3Xin Zhou4Yi Zhang5Mingyun Tang6Juan Fu7Yuqing Deng8Xin Liang9Long Zhao10Zhi Li11Zhi Li12Zhi Li13Tiangang Wang14Li Li15Guohui Xiao16School of Integrated Traditional Chinese and Western Medicine, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Spleen and Stomach Diseases, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, Sichuan, ChinaSchool of Integrated Traditional Chinese and Western Medicine, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Spleen and Stomach Diseases, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, Sichuan, ChinaThe Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Digestive System Diseases of Luzhou city, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Gastroenterological Surgery, Leshan Hospital of Traditional Chinese Medicine, Leshan, Sichuan, ChinaSchool of Integrated Traditional Chinese and Western Medicine, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Spleen and Stomach Diseases, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, Sichuan, ChinaSchool of Integrated Traditional Chinese and Western Medicine, Southwest Medical University, Luzhou, Sichuan, ChinaSchool of Integrated Traditional Chinese and Western Medicine, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Spleen and Stomach Diseases, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Spleen and Stomach Diseases, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, Sichuan, ChinaThe Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Digestive System Diseases of Luzhou city, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, Sichuan, ChinaSchool of Integrated Traditional Chinese and Western Clinical Medicine, North Sichuan Medical College, Nanchong, Sichuan, ChinaDepartment of Spleen and Stomach Diseases, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Spleen and Stomach Diseases, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, Sichuan, ChinaDepartment of Spleen and Stomach Diseases, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, Sichuan, ChinaBackgroundDuring severe acute pancreatitis (SAP), damage to the intestinal mucosal barrier and translocation of intestinal pathogenic bacteria are key mechanisms that accelerate the disease progression of SAP. Chaihuang Qingyi Huoxue Granule (CH) is a herbal formula used in the clinical treatment of SAP. This study aims to investigate the role of CH in regulating gut microbiota and intestinal mucosal barrier in SAP rats.MethodsSodium taurocholate (3.5%) was retrogradely perfused into the biliopancreatic duct to establish the model of SAP in rats. CH (4.4 g/kg) was administered by gavage. Serum amylase, lipase, and endotoxin levels were measured. Hematoxylin-eosin (HE) staining was used to observe morphological changes in the pancreas and colon. The expression of zona occludens-1 (ZO-1) and occludin in the colon was examined by immunohistochemistry (IHC) and western blot. 16S rDNA gene sequencing was used to analyze the gut microbiota of the rats. The content of short-chain fatty acids (SCFAs) in the intestinal contents of the rats was determined by gas chromatography-mass spectrometry (GC-MS).ResultsCH reduced serum amylase, lipase, and endotoxin levels in SAP rats, alleviated pathological damage in the pancreas and colon, and restored the expression of ZO-1 and occludin. Moreover, CH alleviated gut microbiota dysbiosis in SAP rats, with restored gut microbiota diversity and structure. At the phylum level, the relative abundance of Firmicutes and Bacteroidetes increased, while that of Proteobacteria decreased. At the genus level, the abundance of Ruminococcus 1, Parabacteroides, Prevotellaceae UCG-001, Lachnospiraceae NK4A136 group, and Lactobacillus increased, while that of Escherichia-Shigella, Enterococcus, and Enterobacter decreased. In addition, CH increased the levels of SCFAs in the intestinal contents of SAP rats.ConclusionCH ameliorates SAP by maintaining the homeostasis and diversity of the gut microbiota, increasing the levels of SCFAs, and repairing the intestinal mucosal barrier.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1514201/fullsevere acute pancreatitistraditional Chinese medicineChaihuang Qingyi Huoxue Granulegut microbiotaintestinal mucosal barriershort-chain fatty acids |
| spellingShingle | Xiaobin Zhang Xusen Zeng Wen Guo Xin Zhou Xin Zhou Yi Zhang Mingyun Tang Juan Fu Yuqing Deng Xin Liang Long Zhao Zhi Li Zhi Li Zhi Li Tiangang Wang Li Li Guohui Xiao Chaihuang Qingyi Huoxue granule ameliorates severe acute pancreatitis by modulating gut microbiota and repairing the intestinal mucosal barrier Frontiers in Cellular and Infection Microbiology severe acute pancreatitis traditional Chinese medicine Chaihuang Qingyi Huoxue Granule gut microbiota intestinal mucosal barrier short-chain fatty acids |
| title | Chaihuang Qingyi Huoxue granule ameliorates severe acute pancreatitis by modulating gut microbiota and repairing the intestinal mucosal barrier |
| title_full | Chaihuang Qingyi Huoxue granule ameliorates severe acute pancreatitis by modulating gut microbiota and repairing the intestinal mucosal barrier |
| title_fullStr | Chaihuang Qingyi Huoxue granule ameliorates severe acute pancreatitis by modulating gut microbiota and repairing the intestinal mucosal barrier |
| title_full_unstemmed | Chaihuang Qingyi Huoxue granule ameliorates severe acute pancreatitis by modulating gut microbiota and repairing the intestinal mucosal barrier |
| title_short | Chaihuang Qingyi Huoxue granule ameliorates severe acute pancreatitis by modulating gut microbiota and repairing the intestinal mucosal barrier |
| title_sort | chaihuang qingyi huoxue granule ameliorates severe acute pancreatitis by modulating gut microbiota and repairing the intestinal mucosal barrier |
| topic | severe acute pancreatitis traditional Chinese medicine Chaihuang Qingyi Huoxue Granule gut microbiota intestinal mucosal barrier short-chain fatty acids |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1514201/full |
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