OPTIMISATION OF A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY METHOD FOR CATECHOLAMINES AND RELATED MOLECULES ANALYSIS
A simple and specific method for the analysis of 11 compounds (catecholamines, their precursors and their metabolites) has been developed using hydrophilic interaction chromatography. Adrenaline, noradrenalin, dopamine, serotonin, 3,4-dihydroxy-phenylalanine, 3-methoxytyramine, tryptophan, homovanil...
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Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2017-03-01
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| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
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| Online Access: | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201701&vol=1&aid=4487 |
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| author | RALUCA-IOANA CHIRITA-TAMPU ADRIANA FINARU CLAIRE ELFAKIR |
| author_facet | RALUCA-IOANA CHIRITA-TAMPU ADRIANA FINARU CLAIRE ELFAKIR |
| author_sort | RALUCA-IOANA CHIRITA-TAMPU |
| collection | DOAJ |
| description | A simple and specific method for the analysis of 11 compounds (catecholamines, their precursors and their metabolites) has been developed using hydrophilic interaction chromatography. Adrenaline, noradrenalin, dopamine, serotonin, 3,4-dihydroxy-phenylalanine, 3-methoxytyramine, tryptophan, homovanillic acid, tyrosine, 3,4-dihydroxy-phenylacetic acid, 5-hydroxyindole-3-acetic acid and 3,4-dihydroxybenzylalanine (as internal standard) were separated on a TSK gel amide 80 column. The influence of parameters such as organic modifier type and content, salt nature and concentration, pH as well as column temperature on the selectivity were investigated. The optimized mobile phase consisted of a 20 mM ammonium acetate aqueous solution buffered at pH 3 and acetonitrile (20:80 v/v) mixture. |
| format | Article |
| id | doaj-art-abb9f636364443bfabfe54671d3df5f4 |
| institution | OA Journals |
| issn | 1582-540X 1582-540X |
| language | English |
| publishDate | 2017-03-01 |
| publisher | Alma Mater Publishing House "Vasile Alecsandri" University of Bacau |
| record_format | Article |
| series | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| spelling | doaj-art-abb9f636364443bfabfe54671d3df5f42025-08-20T02:20:13ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauScientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry1582-540X1582-540X2017-03-011813141OPTIMISATION OF A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY METHOD FOR CATECHOLAMINES AND RELATED MOLECULES ANALYSISRALUCA-IOANA CHIRITA-TAMPU 0ADRIANA FINARU1CLAIRE ELFAKIR21. ”Vasile Alecsandri” University of Bacau, 157, Calea Marasesti, 600115, Bacau, Romania 2. Institute of Organic and Analytical Chemistry, CNRS UMR 7311, University of Orleans, BP 6759, 45067 Orléans, France ”Vasile Alecsandri” University of Bacau, 157, Calea Marasesti, 600115, Bacau, Romania Institute of Organic and Analytical Chemistry, CNRS UMR 7311, University of Orleans, BP 6759, 45067 Orléans, FranceA simple and specific method for the analysis of 11 compounds (catecholamines, their precursors and their metabolites) has been developed using hydrophilic interaction chromatography. Adrenaline, noradrenalin, dopamine, serotonin, 3,4-dihydroxy-phenylalanine, 3-methoxytyramine, tryptophan, homovanillic acid, tyrosine, 3,4-dihydroxy-phenylacetic acid, 5-hydroxyindole-3-acetic acid and 3,4-dihydroxybenzylalanine (as internal standard) were separated on a TSK gel amide 80 column. The influence of parameters such as organic modifier type and content, salt nature and concentration, pH as well as column temperature on the selectivity were investigated. The optimized mobile phase consisted of a 20 mM ammonium acetate aqueous solution buffered at pH 3 and acetonitrile (20:80 v/v) mixture.http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201701&vol=1&aid=4487catecholamineHILIC optimizationindolamineneurotransmittersTSK gel Amide 80 |
| spellingShingle | RALUCA-IOANA CHIRITA-TAMPU ADRIANA FINARU CLAIRE ELFAKIR OPTIMISATION OF A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY METHOD FOR CATECHOLAMINES AND RELATED MOLECULES ANALYSIS Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry catecholamine HILIC optimization indolamine neurotransmitters TSK gel Amide 80 |
| title | OPTIMISATION OF A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY METHOD FOR CATECHOLAMINES AND RELATED MOLECULES ANALYSIS |
| title_full | OPTIMISATION OF A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY METHOD FOR CATECHOLAMINES AND RELATED MOLECULES ANALYSIS |
| title_fullStr | OPTIMISATION OF A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY METHOD FOR CATECHOLAMINES AND RELATED MOLECULES ANALYSIS |
| title_full_unstemmed | OPTIMISATION OF A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY METHOD FOR CATECHOLAMINES AND RELATED MOLECULES ANALYSIS |
| title_short | OPTIMISATION OF A HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY METHOD FOR CATECHOLAMINES AND RELATED MOLECULES ANALYSIS |
| title_sort | optimisation of a hydrophilic interaction liquid chromatography method for catecholamines and related molecules analysis |
| topic | catecholamine HILIC optimization indolamine neurotransmitters TSK gel Amide 80 |
| url | http://pubs.ub.ro/?pg=revues&rev=cscc6&num=201701&vol=1&aid=4487 |
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