Relevance of TNF-α, IL-6 and IRAK1 gene expression for assessing disease severity and therapy effects in tuberculosis patients
Introduction: Tuberculosis (TBC) is a contagious chronic respiratory disease which despite the known cause, Mycobacterium tuberculosis (Mtb), and many decades of successful therapy, remains one of the leading global health problems. Immune responses against Mtb infection involve both of types of im...
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The Journal of Infection in Developing Countries
2019-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/10949 |
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| author | Ivana Buha Vesna Škodrić-Trifunović Tatjana Adžić-Vukičević Aleksandra Ilić Ana Blanka-Protić Mihailo Stjepanovic Marina Anđelković Miša Vreća Jelena Milin-Lazović Vesna Spasovski Sonja Pavlović |
| author_facet | Ivana Buha Vesna Škodrić-Trifunović Tatjana Adžić-Vukičević Aleksandra Ilić Ana Blanka-Protić Mihailo Stjepanovic Marina Anđelković Miša Vreća Jelena Milin-Lazović Vesna Spasovski Sonja Pavlović |
| author_sort | Ivana Buha |
| collection | DOAJ |
| description |
Introduction: Tuberculosis (TBC) is a contagious chronic respiratory disease which despite the known cause, Mycobacterium tuberculosis (Mtb), and many decades of successful therapy, remains one of the leading global health problems. Immune responses against Mtb infection involve both of types of immunity, but cellular immunity, in which certain cytokines and Th1 cells play a key role, is crucial. A better understanding of the functions of the cytokine network involved in the state and progression of TBC could identify specific molecular markers for monitoring of disease activity as well as therapy outcomes in TBC patients.
Methodology: We investigated expression of TNF-α, IL-6 and IRAK1 genes using an RT-qPCR technique in peripheral blood mononuclear cells of 33 TBC patients and 10 healthy individuals.
Results: Comparison between TBC patients and healthy individuals revealed statistically significant differences for all analyzed genes. The levels of expression of TNF-α and IL-6 mRNA were higher, while the level of IRAK1 mRNA was lower in the TBC group compared to controls. Moreover, a strong positive correlation was observed between TNF-α and IL-6 gene expression. When clinical parameters were analyzed, increased levels of TNF-α mRNA were detected in patients with a longer duration of therapy (>2 months) compared to those with a shorter therapy duration (< 2 months), and in patients without anemia.
Conclusions: Our results indicate that the inflammatory genes we examined play a crucial role in the pathogenesis of tuberculosis, and that the expression of the TNF-α gene could be a marker for monitoring the clinical effect of the ant-tuberculosis drugs during therapy.
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| format | Article |
| id | doaj-art-abb34114cb8640f7afa5aee4de582a3e |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2019-05-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-abb34114cb8640f7afa5aee4de582a3e2025-08-20T02:27:09ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802019-05-01130510.3855/jidc.10949Relevance of TNF-α, IL-6 and IRAK1 gene expression for assessing disease severity and therapy effects in tuberculosis patientsIvana Buha0Vesna Škodrić-Trifunović1Tatjana Adžić-Vukičević2Aleksandra Ilić3Ana Blanka-Protić4Mihailo Stjepanovic5Marina Anđelković6Miša Vreća7Jelena Milin-Lazović8Vesna Spasovski9Sonja Pavlović10Clinic of Pulmonology, Clinical Centre of Serbia, Belgrade, SerbiaClinic of Pulmonology, Clinical Centre of Serbia, Belgrade, SerbiaClinic of Pulmonology, Clinical Centre of Serbia, Belgrade, SerbiaClinic of Pulmonology, Clinical Centre of Serbia, Belgrade, SerbiaClinic of Pulmonology, Clinical Centre of Serbia, Belgrade, SerbiaClinic of Pulmonology, Clinical Centre of Serbia, Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, SerbiaSchool of Medicine, University of Belgrade, Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, SerbiaInstitute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia Introduction: Tuberculosis (TBC) is a contagious chronic respiratory disease which despite the known cause, Mycobacterium tuberculosis (Mtb), and many decades of successful therapy, remains one of the leading global health problems. Immune responses against Mtb infection involve both of types of immunity, but cellular immunity, in which certain cytokines and Th1 cells play a key role, is crucial. A better understanding of the functions of the cytokine network involved in the state and progression of TBC could identify specific molecular markers for monitoring of disease activity as well as therapy outcomes in TBC patients. Methodology: We investigated expression of TNF-α, IL-6 and IRAK1 genes using an RT-qPCR technique in peripheral blood mononuclear cells of 33 TBC patients and 10 healthy individuals. Results: Comparison between TBC patients and healthy individuals revealed statistically significant differences for all analyzed genes. The levels of expression of TNF-α and IL-6 mRNA were higher, while the level of IRAK1 mRNA was lower in the TBC group compared to controls. Moreover, a strong positive correlation was observed between TNF-α and IL-6 gene expression. When clinical parameters were analyzed, increased levels of TNF-α mRNA were detected in patients with a longer duration of therapy (>2 months) compared to those with a shorter therapy duration (< 2 months), and in patients without anemia. Conclusions: Our results indicate that the inflammatory genes we examined play a crucial role in the pathogenesis of tuberculosis, and that the expression of the TNF-α gene could be a marker for monitoring the clinical effect of the ant-tuberculosis drugs during therapy. https://jidc.org/index.php/journal/article/view/10949gene expressionmonitoring therapypulmonary tuberculosiscytokines |
| spellingShingle | Ivana Buha Vesna Škodrić-Trifunović Tatjana Adžić-Vukičević Aleksandra Ilić Ana Blanka-Protić Mihailo Stjepanovic Marina Anđelković Miša Vreća Jelena Milin-Lazović Vesna Spasovski Sonja Pavlović Relevance of TNF-α, IL-6 and IRAK1 gene expression for assessing disease severity and therapy effects in tuberculosis patients Journal of Infection in Developing Countries gene expression monitoring therapy pulmonary tuberculosis cytokines |
| title | Relevance of TNF-α, IL-6 and IRAK1 gene expression for assessing disease severity and therapy effects in tuberculosis patients |
| title_full | Relevance of TNF-α, IL-6 and IRAK1 gene expression for assessing disease severity and therapy effects in tuberculosis patients |
| title_fullStr | Relevance of TNF-α, IL-6 and IRAK1 gene expression for assessing disease severity and therapy effects in tuberculosis patients |
| title_full_unstemmed | Relevance of TNF-α, IL-6 and IRAK1 gene expression for assessing disease severity and therapy effects in tuberculosis patients |
| title_short | Relevance of TNF-α, IL-6 and IRAK1 gene expression for assessing disease severity and therapy effects in tuberculosis patients |
| title_sort | relevance of tnf α il 6 and irak1 gene expression for assessing disease severity and therapy effects in tuberculosis patients |
| topic | gene expression monitoring therapy pulmonary tuberculosis cytokines |
| url | https://jidc.org/index.php/journal/article/view/10949 |
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