Chikungunya Particle and RNA Induce Mechanical and Heat Hypersensitivities in a TRPV1-Dependent Manner
Chikungunya virus (CHIKV), the causative agent of the chikungunya fever, is an alphavirus widely transmitted by the bite of the female mosquito of the genus <i>Aedes</i> sp., especially in tropical and subtropical regions. Brazil is the country most affected by the microorganism. CHIKV c...
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2025-01-01
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| author | Liziane C. M. da Silva Andressa C. dos Santos Maia Nágila C. F. de Sousa Catielen P. Pavi Beatriz P. Savi Seigo Nagashima Samara Damasceno Ayda H. Schneider Lucas Z. Mascarin João F. S. Rodrigues Cinara R. A. V. Monteiro Izabella T. Silva Gislaine Fongaro Valério Monteiro-Neto Maria R. Q. Bomfim Thiago M. Cunha João de Sousa Valente João B. Calixto Lúcia de Noronha Susan D. Brain Elizabeth S. Fernandes |
| author_facet | Liziane C. M. da Silva Andressa C. dos Santos Maia Nágila C. F. de Sousa Catielen P. Pavi Beatriz P. Savi Seigo Nagashima Samara Damasceno Ayda H. Schneider Lucas Z. Mascarin João F. S. Rodrigues Cinara R. A. V. Monteiro Izabella T. Silva Gislaine Fongaro Valério Monteiro-Neto Maria R. Q. Bomfim Thiago M. Cunha João de Sousa Valente João B. Calixto Lúcia de Noronha Susan D. Brain Elizabeth S. Fernandes |
| author_sort | Liziane C. M. da Silva |
| collection | DOAJ |
| description | Chikungunya virus (CHIKV), the causative agent of the chikungunya fever, is an alphavirus widely transmitted by the bite of the female mosquito of the genus <i>Aedes</i> sp., especially in tropical and subtropical regions. Brazil is the country most affected by the microorganism. CHIKV classically induces articular pain, which can become long lasting for even years in a great number of the infected individuals, reducing their quality of life. The mechanisms of CHIKV-induced pain are poorly understood, but recent evidence indicated a role for the transient receptor potential vanilloid 1 (TRPV1) in this pathology. Herein, we assessed the ability of intra-articularly injected inactivated CHIKV or its RNA to trigger nociception in mice. Both stimuli induced bilateral secondary hyperalgesia to mechanical and heat stimuli. These responses were attenuated by TRPV1 ablation or antagonism. Joint structural alterations and increased cartilage TRPV1 protein expression were detected in the ipsilateral knee joints injected with either CHIKV or viral RNA. However, the lack of this receptor did not influence the histological changes triggered by CHIKV or RNA. The results further support the role of TRPV1 in CHIKV-induced pain and highlight its importance in the chronic phase of the disease. |
| format | Article |
| id | doaj-art-ab982050d47e4fa490b6c5a85b4e32eb |
| institution | DOAJ |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-01-01 |
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| series | Biomolecules |
| spelling | doaj-art-ab982050d47e4fa490b6c5a85b4e32eb2025-08-20T02:44:36ZengMDPI AGBiomolecules2218-273X2025-01-0115217110.3390/biom15020171Chikungunya Particle and RNA Induce Mechanical and Heat Hypersensitivities in a TRPV1-Dependent MannerLiziane C. M. da Silva0Andressa C. dos Santos Maia1Nágila C. F. de Sousa2Catielen P. Pavi3Beatriz P. Savi4Seigo Nagashima5Samara Damasceno6Ayda H. Schneider7Lucas Z. Mascarin8João F. S. Rodrigues9Cinara R. A. V. Monteiro10Izabella T. Silva11Gislaine Fongaro12Valério Monteiro-Neto13Maria R. Q. Bomfim14Thiago M. Cunha15João de Sousa Valente16João B. Calixto17Lúcia de Noronha18Susan D. Brain19Elizabeth S. Fernandes20Programa de Pós-Graduação em Biotecnologia Aplicada à Saúde da Criança e do Adolescente, Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, BrazilPrograma de Pós-Graduação em Biotecnologia Aplicada à Saúde da Criança e do Adolescente, Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, BrazilPrograma de Pós-Graduação, Universidade CEUMA, São Luís 65075-120, MA, BrazilDepartamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, BrazilDepartamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, BrazilEscola de Medicina e Ciências da Vida, Pontifícia Universidade Católica do Paraná, Curitiba 80215-901, PR, BrazilFaculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14049-900, SP, BrazilFaculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14049-900, SP, BrazilDepartamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, BrazilPrograma de Pós-Graduação, Universidade CEUMA, São Luís 65075-120, MA, BrazilPrograma de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, São Luís 65085-040, MA, BrazilDepartamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, BrazilDepartamento de Microbiologia, Imunologia e Parasitologia, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, BrazilPrograma de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, São Luís 65085-040, MA, BrazilPrograma de Pós-Graduação, Universidade CEUMA, São Luís 65075-120, MA, BrazilFaculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto 14049-900, SP, BrazilThe School of Cardiovascular and Metabolic Medicine (South Bank), King’s College London, London SE1 9NH, UKCentro de Inovação e Ensaios Pré-Clínicos, Florianópolis 88056-000, SC, BrazilEscola de Medicina e Ciências da Vida, Pontifícia Universidade Católica do Paraná, Curitiba 80215-901, PR, BrazilThe School of Cardiovascular and Metabolic Medicine (South Bank), King’s College London, London SE1 9NH, UKPrograma de Pós-Graduação em Biotecnologia Aplicada à Saúde da Criança e do Adolescente, Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, BrazilChikungunya virus (CHIKV), the causative agent of the chikungunya fever, is an alphavirus widely transmitted by the bite of the female mosquito of the genus <i>Aedes</i> sp., especially in tropical and subtropical regions. Brazil is the country most affected by the microorganism. CHIKV classically induces articular pain, which can become long lasting for even years in a great number of the infected individuals, reducing their quality of life. The mechanisms of CHIKV-induced pain are poorly understood, but recent evidence indicated a role for the transient receptor potential vanilloid 1 (TRPV1) in this pathology. Herein, we assessed the ability of intra-articularly injected inactivated CHIKV or its RNA to trigger nociception in mice. Both stimuli induced bilateral secondary hyperalgesia to mechanical and heat stimuli. These responses were attenuated by TRPV1 ablation or antagonism. Joint structural alterations and increased cartilage TRPV1 protein expression were detected in the ipsilateral knee joints injected with either CHIKV or viral RNA. However, the lack of this receptor did not influence the histological changes triggered by CHIKV or RNA. The results further support the role of TRPV1 in CHIKV-induced pain and highlight its importance in the chronic phase of the disease.https://www.mdpi.com/2218-273X/15/2/171Chikungunyaviral RNAarthralgiaTRPV1nociception |
| spellingShingle | Liziane C. M. da Silva Andressa C. dos Santos Maia Nágila C. F. de Sousa Catielen P. Pavi Beatriz P. Savi Seigo Nagashima Samara Damasceno Ayda H. Schneider Lucas Z. Mascarin João F. S. Rodrigues Cinara R. A. V. Monteiro Izabella T. Silva Gislaine Fongaro Valério Monteiro-Neto Maria R. Q. Bomfim Thiago M. Cunha João de Sousa Valente João B. Calixto Lúcia de Noronha Susan D. Brain Elizabeth S. Fernandes Chikungunya Particle and RNA Induce Mechanical and Heat Hypersensitivities in a TRPV1-Dependent Manner Biomolecules Chikungunya viral RNA arthralgia TRPV1 nociception |
| title | Chikungunya Particle and RNA Induce Mechanical and Heat Hypersensitivities in a TRPV1-Dependent Manner |
| title_full | Chikungunya Particle and RNA Induce Mechanical and Heat Hypersensitivities in a TRPV1-Dependent Manner |
| title_fullStr | Chikungunya Particle and RNA Induce Mechanical and Heat Hypersensitivities in a TRPV1-Dependent Manner |
| title_full_unstemmed | Chikungunya Particle and RNA Induce Mechanical and Heat Hypersensitivities in a TRPV1-Dependent Manner |
| title_short | Chikungunya Particle and RNA Induce Mechanical and Heat Hypersensitivities in a TRPV1-Dependent Manner |
| title_sort | chikungunya particle and rna induce mechanical and heat hypersensitivities in a trpv1 dependent manner |
| topic | Chikungunya viral RNA arthralgia TRPV1 nociception |
| url | https://www.mdpi.com/2218-273X/15/2/171 |
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