Erythromycin effect on the intestinal microbial community of rats
The effect of erythromycin on the intestinal microbial community of laboratory rats was studied. The research is important for the practical purposes of animal farming and veterinary medicine, because a significant increase in the abundance of pathogenic microorganisms caused by antibiotic treatment...
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| Format: | Article |
| Language: | English |
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Kazan Federal University
2020-03-01
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| Series: | Учёные записки Казанского университета: Серия Естественные науки |
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| Online Access: | https://kpfu.ru/uz-eng-ns-2020-1-7.html |
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| author | E.V. Skvortsov Rish.S. Muhammadiev Rin.S. Muhammadiev L.R. Valiullin |
| author_facet | E.V. Skvortsov Rish.S. Muhammadiev Rin.S. Muhammadiev L.R. Valiullin |
| author_sort | E.V. Skvortsov |
| collection | DOAJ |
| description | The effect of erythromycin on the intestinal microbial community of laboratory rats was studied. The research is important for the practical purposes of animal farming and veterinary medicine, because a significant increase in the abundance of pathogenic microorganisms caused by antibiotic treatment turns the intestine into a reservoir of infections resistant to antibiotics, which need to be combated efficiently. To solve this problem, both rapid and accurate methods must be elaborated for quantitative determination of the main bacteria in the intestine during the use of antibiotics, such as erythromycin. We used genetic analysis (real-time polymerase chain reaction) to study the intestinal microflora of male rats aged 8 weeks. All rats were divided into two groups: the control group (n = 10); the experimental group (n = 10) that received orally administered erythromycin. The abundance of the main intestinal bacteria was determined from fecal samples. The content of several variations of erm (erythromycin resistance) genes in the intestinal microflora was also investigated. The obtained results demonstrate that erythromycin causes a significant increase in the abundance of Clostridium difficile and Staphylococcus aureus, as well as in the number of erm genes. These data can be considered a further advance in our understanding of prospects and consequences of antibiotic therapy in animal farming and veterinary medicine. |
| format | Article |
| id | doaj-art-ab96e6d1d8ce442ea0d4092b2ce64674 |
| institution | DOAJ |
| issn | 2542-064X 2500-218X |
| language | English |
| publishDate | 2020-03-01 |
| publisher | Kazan Federal University |
| record_format | Article |
| series | Учёные записки Казанского университета: Серия Естественные науки |
| spelling | doaj-art-ab96e6d1d8ce442ea0d4092b2ce646742025-08-20T02:42:56ZengKazan Federal UniversityУчёные записки Казанского университета: Серия Естественные науки2542-064X2500-218X2020-03-01162111212210.26907/2542-064X.2020.1.112-122Erythromycin effect on the intestinal microbial community of ratsE.V. Skvortsov0Rish.S. Muhammadiev1Rin.S. Muhammadiev2L.R. Valiullin3Federal Center of Toxicological, Radiation, and Biological Safety – All-Russian Research Veterinary Institute, Kazan, 420075 RussiaFederal Center of Toxicological, Radiation, and Biological Safety – All-Russian Research Veterinary Institute, Kazan, 420075 RussiaFederal Center of Toxicological, Radiation, and Biological Safety – All-Russian Research Veterinary Institute, Kazan, 420075 RussiaFederal Center of Toxicological, Radiation, and Biological Safety – All-Russian Research Veterinary Institute, Kazan, 420075 RussiaThe effect of erythromycin on the intestinal microbial community of laboratory rats was studied. The research is important for the practical purposes of animal farming and veterinary medicine, because a significant increase in the abundance of pathogenic microorganisms caused by antibiotic treatment turns the intestine into a reservoir of infections resistant to antibiotics, which need to be combated efficiently. To solve this problem, both rapid and accurate methods must be elaborated for quantitative determination of the main bacteria in the intestine during the use of antibiotics, such as erythromycin. We used genetic analysis (real-time polymerase chain reaction) to study the intestinal microflora of male rats aged 8 weeks. All rats were divided into two groups: the control group (n = 10); the experimental group (n = 10) that received orally administered erythromycin. The abundance of the main intestinal bacteria was determined from fecal samples. The content of several variations of erm (erythromycin resistance) genes in the intestinal microflora was also investigated. The obtained results demonstrate that erythromycin causes a significant increase in the abundance of Clostridium difficile and Staphylococcus aureus, as well as in the number of erm genes. These data can be considered a further advance in our understanding of prospects and consequences of antibiotic therapy in animal farming and veterinary medicine.https://kpfu.ru/uz-eng-ns-2020-1-7.htmlintestinal microbial communityerythromycingenetic analysiserythromycin resistance genes |
| spellingShingle | E.V. Skvortsov Rish.S. Muhammadiev Rin.S. Muhammadiev L.R. Valiullin Erythromycin effect on the intestinal microbial community of rats Учёные записки Казанского университета: Серия Естественные науки intestinal microbial community erythromycin genetic analysis erythromycin resistance genes |
| title | Erythromycin effect on the intestinal microbial community of rats |
| title_full | Erythromycin effect on the intestinal microbial community of rats |
| title_fullStr | Erythromycin effect on the intestinal microbial community of rats |
| title_full_unstemmed | Erythromycin effect on the intestinal microbial community of rats |
| title_short | Erythromycin effect on the intestinal microbial community of rats |
| title_sort | erythromycin effect on the intestinal microbial community of rats |
| topic | intestinal microbial community erythromycin genetic analysis erythromycin resistance genes |
| url | https://kpfu.ru/uz-eng-ns-2020-1-7.html |
| work_keys_str_mv | AT evskvortsov erythromycineffectontheintestinalmicrobialcommunityofrats AT rishsmuhammadiev erythromycineffectontheintestinalmicrobialcommunityofrats AT rinsmuhammadiev erythromycineffectontheintestinalmicrobialcommunityofrats AT lrvaliullin erythromycineffectontheintestinalmicrobialcommunityofrats |