In vitro slow-growth conservation, acclimatization, and genetic stability of virus-free apple plants
Abstract In vitro slow-growth storage has long played an important role in maintaining valuable horticultural materials. It is particularly applicable to the conservation of virus-free materials recovered from meristem culture or shoot-tip cryotherapy. In this study, the apple cultivar ‘Yanfu-6’ and...
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| Language: | English |
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Springer
2024-11-01
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| Series: | Horticulture Advances |
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| Online Access: | https://doi.org/10.1007/s44281-024-00049-0 |
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| author | Xian Lu Pengpeng Sun Ruihan Liu Caiwen Wang Lu Tong Muhammad Mobeen Tahir Xiaoyan Ma Junhua Bao Dong Zhang Minrui Wang Na An |
| author_facet | Xian Lu Pengpeng Sun Ruihan Liu Caiwen Wang Lu Tong Muhammad Mobeen Tahir Xiaoyan Ma Junhua Bao Dong Zhang Minrui Wang Na An |
| author_sort | Xian Lu |
| collection | DOAJ |
| description | Abstract In vitro slow-growth storage has long played an important role in maintaining valuable horticultural materials. It is particularly applicable to the conservation of virus-free materials recovered from meristem culture or shoot-tip cryotherapy. In this study, the apple cultivar ‘Yanfu-6’ and the rootstock genotype ‘Qingzhen-1’ obtained from a virus disinfection program were compared during the establishment of in vitro slow-growth storage programs. At room temperature (25℃), combining with 4.5% sucrose or 0.5% mannitol, extended the conservation period of ‘Yanfu-6’ and ‘Qingzhen-1’to 5 and 9 months, respectively. Decreasing the temperature to 12℃ led to further reduced shoot growth, extending the conservation period to 9 months for ‘Yanfu-6’, while more than 80% of ‘Qingzhen-1’ shoots could be recovered after one year of storage. Similarly, high rooting and acclimatization success levels were obtained for ‘Qingzhen-1’ after one-year storage at 12℃, as well as for the plants that underwent monthly subcultures, but not for ‘Yanfu-6’. The inability to root in ‘Yanfu-6’ was overcome by micrografting onto rootstock ‘Qingzhen-1’, which resulted in a rooting percentage of 83% and an acclimatization success of 77%. In the analysis of genetic stability by next-generation sequencing, reduced levels of single nucleotide polymorphism (SNPs) and insertions and deletions (InDels) were detected in ‘Qingzhen-1’ shoots recovered after one-year storage at 12℃, as compared with shoots that underwent regular subcultures. These results highlight the use of in vitro slow-growth program assisted with micrografting for the conservation of valuable horticultural species. |
| format | Article |
| id | doaj-art-ab941d2a8eba4157ac51d4bfe1a7bd1f |
| institution | OA Journals |
| issn | 2948-1104 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Springer |
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| series | Horticulture Advances |
| spelling | doaj-art-ab941d2a8eba4157ac51d4bfe1a7bd1f2025-08-20T02:22:20ZengSpringerHorticulture Advances2948-11042024-11-012111210.1007/s44281-024-00049-0In vitro slow-growth conservation, acclimatization, and genetic stability of virus-free apple plantsXian Lu0Pengpeng Sun1Ruihan Liu2Caiwen Wang3Lu Tong4Muhammad Mobeen Tahir5Xiaoyan Ma6Junhua Bao7Dong Zhang8Minrui Wang9Na An10College of Horticulture, Yangling Sub-Center of National Center for Apple Improvement, Northwest A&F UniversityCollege of Horticulture, Yangling Sub-Center of National Center for Apple Improvement, Northwest A&F UniversityCollege of Life Science, Northwest A&F UniversityCollege of Life Science, Northwest A&F UniversityCollege of Horticulture, Yangling Sub-Center of National Center for Apple Improvement, Northwest A&F UniversityCollege of Horticulture, Yangling Sub-Center of National Center for Apple Improvement, Northwest A&F UniversityCollege of Horticulture, Yangling Sub-Center of National Center for Apple Improvement, Northwest A&F UniversityCollege of Horticulture, Yangling Sub-Center of National Center for Apple Improvement, Northwest A&F UniversityCollege of Horticulture, Yangling Sub-Center of National Center for Apple Improvement, Northwest A&F UniversityCollege of Horticulture, Yangling Sub-Center of National Center for Apple Improvement, Northwest A&F UniversityCollege of Horticulture, Yangling Sub-Center of National Center for Apple Improvement, Northwest A&F UniversityAbstract In vitro slow-growth storage has long played an important role in maintaining valuable horticultural materials. It is particularly applicable to the conservation of virus-free materials recovered from meristem culture or shoot-tip cryotherapy. In this study, the apple cultivar ‘Yanfu-6’ and the rootstock genotype ‘Qingzhen-1’ obtained from a virus disinfection program were compared during the establishment of in vitro slow-growth storage programs. At room temperature (25℃), combining with 4.5% sucrose or 0.5% mannitol, extended the conservation period of ‘Yanfu-6’ and ‘Qingzhen-1’to 5 and 9 months, respectively. Decreasing the temperature to 12℃ led to further reduced shoot growth, extending the conservation period to 9 months for ‘Yanfu-6’, while more than 80% of ‘Qingzhen-1’ shoots could be recovered after one year of storage. Similarly, high rooting and acclimatization success levels were obtained for ‘Qingzhen-1’ after one-year storage at 12℃, as well as for the plants that underwent monthly subcultures, but not for ‘Yanfu-6’. The inability to root in ‘Yanfu-6’ was overcome by micrografting onto rootstock ‘Qingzhen-1’, which resulted in a rooting percentage of 83% and an acclimatization success of 77%. In the analysis of genetic stability by next-generation sequencing, reduced levels of single nucleotide polymorphism (SNPs) and insertions and deletions (InDels) were detected in ‘Qingzhen-1’ shoots recovered after one-year storage at 12℃, as compared with shoots that underwent regular subcultures. These results highlight the use of in vitro slow-growth program assisted with micrografting for the conservation of valuable horticultural species.https://doi.org/10.1007/s44281-024-00049-0Genetic stabilityMalusMannitolRooting analysisSlow-growth conservation |
| spellingShingle | Xian Lu Pengpeng Sun Ruihan Liu Caiwen Wang Lu Tong Muhammad Mobeen Tahir Xiaoyan Ma Junhua Bao Dong Zhang Minrui Wang Na An In vitro slow-growth conservation, acclimatization, and genetic stability of virus-free apple plants Horticulture Advances Genetic stability Malus Mannitol Rooting analysis Slow-growth conservation |
| title | In vitro slow-growth conservation, acclimatization, and genetic stability of virus-free apple plants |
| title_full | In vitro slow-growth conservation, acclimatization, and genetic stability of virus-free apple plants |
| title_fullStr | In vitro slow-growth conservation, acclimatization, and genetic stability of virus-free apple plants |
| title_full_unstemmed | In vitro slow-growth conservation, acclimatization, and genetic stability of virus-free apple plants |
| title_short | In vitro slow-growth conservation, acclimatization, and genetic stability of virus-free apple plants |
| title_sort | in vitro slow growth conservation acclimatization and genetic stability of virus free apple plants |
| topic | Genetic stability Malus Mannitol Rooting analysis Slow-growth conservation |
| url | https://doi.org/10.1007/s44281-024-00049-0 |
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