Potential application of α-amylase from germinating cucurbita moschata duchesne seeds in eco-friendly bioethanol production
Abstract The α-amylase from Cucurbita moschata Duchesne seeds (CmAmy) was purified using ammonium sulfate fractionation, followed by β-cyclodextrin sepharose 6B affinity chromatography. The enzyme exhibited a specific activity of 475.00 U/mg, resulting in a 208.54-fold and a 30.89% yield. The optima...
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Nature Portfolio
2025-07-01
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| author | Saijai Posoongnoen Theera Thummavongsa Jatupol Junthip Jinda Jandaruang Sutthidech Preecharram |
| author_facet | Saijai Posoongnoen Theera Thummavongsa Jatupol Junthip Jinda Jandaruang Sutthidech Preecharram |
| author_sort | Saijai Posoongnoen |
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| description | Abstract The α-amylase from Cucurbita moschata Duchesne seeds (CmAmy) was purified using ammonium sulfate fractionation, followed by β-cyclodextrin sepharose 6B affinity chromatography. The enzyme exhibited a specific activity of 475.00 U/mg, resulting in a 208.54-fold and a 30.89% yield. The optimal pH and temperature for CmAmy were determined to be 7.0 and 60 °C, respectively. The enzyme demonstrated high stability across a temperature range of 40–80 °C and a pH range of 5–9. The CmAmy has been identified as an eco-friendly candidate for bioethanol production by utilizing cassava and rice waste. The optimal condition for sugar production through hydrolysis with CmAmy involved an enzyme concentration of 7.5 mU, a substrate concentration of 2.0% w/v, and a hydrolysis duration of 4 h for cassava waste. For rice waste, the optimal parameters consisted of an enzyme amount of 10 mU, a substrate concentration of 1.5% w/v, and a hydrolysis time of 3 h. The resulting sugars were fermented using Saccharomyces cerevisiae to yield bioethanol, achieving maximum concentrations of 19.6 g/L (0.98 g ethanol/g cassava waste) and 20.1 g/L (1.33 g ethanol/g rice waste) for cassava and rice waste, respectively. This investigation highlights the potential of CmAmy for eco-friendly bioethanol production, offering advantages of simplicity, cost-effectiveness, and sustainability. |
| format | Article |
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| institution | DOAJ |
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| spelling | doaj-art-ab8a97e00735448e8780cc080786bce82025-08-20T03:04:39ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-11653-zPotential application of α-amylase from germinating cucurbita moschata duchesne seeds in eco-friendly bioethanol productionSaijai Posoongnoen0Theera Thummavongsa1Jatupol Junthip2Jinda Jandaruang3Sutthidech Preecharram4Division of Chemistry, Faculty of Science and Technology, Nakhon Ratchasima Rajabhat UniversityDivision of Biology, Faculty of Science and Technology, Nakhon Ratchasima Rajabhat UniversityDivision of Chemistry, Faculty of Science and Technology, Nakhon Ratchasima Rajabhat UniversityChemistry Program, Faculty of Science and Technology, Sakon Nakhon Rajabhat UniversityDepartment of General Science, Faculty of Science and Engineering, Kasetsart University Chalermphrakiat Sakon Nakhon Province CampusAbstract The α-amylase from Cucurbita moschata Duchesne seeds (CmAmy) was purified using ammonium sulfate fractionation, followed by β-cyclodextrin sepharose 6B affinity chromatography. The enzyme exhibited a specific activity of 475.00 U/mg, resulting in a 208.54-fold and a 30.89% yield. The optimal pH and temperature for CmAmy were determined to be 7.0 and 60 °C, respectively. The enzyme demonstrated high stability across a temperature range of 40–80 °C and a pH range of 5–9. The CmAmy has been identified as an eco-friendly candidate for bioethanol production by utilizing cassava and rice waste. The optimal condition for sugar production through hydrolysis with CmAmy involved an enzyme concentration of 7.5 mU, a substrate concentration of 2.0% w/v, and a hydrolysis duration of 4 h for cassava waste. For rice waste, the optimal parameters consisted of an enzyme amount of 10 mU, a substrate concentration of 1.5% w/v, and a hydrolysis time of 3 h. The resulting sugars were fermented using Saccharomyces cerevisiae to yield bioethanol, achieving maximum concentrations of 19.6 g/L (0.98 g ethanol/g cassava waste) and 20.1 g/L (1.33 g ethanol/g rice waste) for cassava and rice waste, respectively. This investigation highlights the potential of CmAmy for eco-friendly bioethanol production, offering advantages of simplicity, cost-effectiveness, and sustainability.https://doi.org/10.1038/s41598-025-11653-zα-AmylasesBioethanolCucurbita moschata Duchesne seedsCassava wasteRice waste |
| spellingShingle | Saijai Posoongnoen Theera Thummavongsa Jatupol Junthip Jinda Jandaruang Sutthidech Preecharram Potential application of α-amylase from germinating cucurbita moschata duchesne seeds in eco-friendly bioethanol production Scientific Reports α-Amylases Bioethanol Cucurbita moschata Duchesne seeds Cassava waste Rice waste |
| title | Potential application of α-amylase from germinating cucurbita moschata duchesne seeds in eco-friendly bioethanol production |
| title_full | Potential application of α-amylase from germinating cucurbita moschata duchesne seeds in eco-friendly bioethanol production |
| title_fullStr | Potential application of α-amylase from germinating cucurbita moschata duchesne seeds in eco-friendly bioethanol production |
| title_full_unstemmed | Potential application of α-amylase from germinating cucurbita moschata duchesne seeds in eco-friendly bioethanol production |
| title_short | Potential application of α-amylase from germinating cucurbita moschata duchesne seeds in eco-friendly bioethanol production |
| title_sort | potential application of α amylase from germinating cucurbita moschata duchesne seeds in eco friendly bioethanol production |
| topic | α-Amylases Bioethanol Cucurbita moschata Duchesne seeds Cassava waste Rice waste |
| url | https://doi.org/10.1038/s41598-025-11653-z |
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