Effect of Water Clover (Marsilea crenata) Ethanol Extracts on Follicle and Oocyte Diameter of Goat: In Vitro Study
Water clover (Marsilea crenata) is one of the herbal plants that has been using in alternative medicine. It possesses pharmacologically active compounds like flavonoid, which has cellular activities such as antioxidant and estrogenic activity. This study aimed to evaluate the impact of water clover...
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University of Brawijaya
2020-06-01
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| Series: | Journal of Experimental Life Science |
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| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/397 |
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| author | Siska Nanda Widhaningrum Septiawan Putranto Sri Rahayu Gatot Ciptadi |
| author_facet | Siska Nanda Widhaningrum Septiawan Putranto Sri Rahayu Gatot Ciptadi |
| author_sort | Siska Nanda Widhaningrum |
| collection | DOAJ |
| description | Water clover (Marsilea crenata) is one of the herbal plants that has been using in alternative medicine. It possesses pharmacologically active compounds like flavonoid, which has cellular activities such as antioxidant and estrogenic activity. This study aimed to evaluate the impact of water clover ethanol extract (WCE) at different concentrations on the growth of follicles and oocytes based on follicles and oocytes diameter, respectively, after six days of culture. This experimental study used 24 isolated antral follicles (2.5-3.2 mm), which were randomly divided into four groups including control (without supplemented WCE) and experimental groups that supplemented with different concentrations of WCE (21.6, 43.2, and 86.4 µg ml-1) in culture medium for six days culture. The diameter of follicles was measured on days 0, 3, and 6. Additionally, oocytes diameter was also measured on day 6. The results indicate that the mean diameter of antral follicles and oocyte diameter of WCE 43.2 µg ml-1 was significantly increase compared to the other groups (P≤0.05). According to our results, WCE exerts its effect on the growth of the antral follicle and oocyte based on follicles and oocytes diameter respectively in a dose-dependent manner after six days of the antral follicle cultured |
| format | Article |
| id | doaj-art-ab800ae3fa5f4f9a8b038cddac30f368 |
| institution | DOAJ |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Experimental Life Science |
| spelling | doaj-art-ab800ae3fa5f4f9a8b038cddac30f3682025-08-20T03:09:57ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552020-06-01102113118https://doi.org/10.21776/ub.jels.2020.010.02.06Effect of Water Clover (Marsilea crenata) Ethanol Extracts on Follicle and Oocyte Diameter of Goat: In Vitro StudySiska Nanda Widhaningrum0Septiawan Putranto1Sri Rahayu2Gatot Ciptadi3Universitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaUniversitas BrawijayaWater clover (Marsilea crenata) is one of the herbal plants that has been using in alternative medicine. It possesses pharmacologically active compounds like flavonoid, which has cellular activities such as antioxidant and estrogenic activity. This study aimed to evaluate the impact of water clover ethanol extract (WCE) at different concentrations on the growth of follicles and oocytes based on follicles and oocytes diameter, respectively, after six days of culture. This experimental study used 24 isolated antral follicles (2.5-3.2 mm), which were randomly divided into four groups including control (without supplemented WCE) and experimental groups that supplemented with different concentrations of WCE (21.6, 43.2, and 86.4 µg ml-1) in culture medium for six days culture. The diameter of follicles was measured on days 0, 3, and 6. Additionally, oocytes diameter was also measured on day 6. The results indicate that the mean diameter of antral follicles and oocyte diameter of WCE 43.2 µg ml-1 was significantly increase compared to the other groups (P≤0.05). According to our results, WCE exerts its effect on the growth of the antral follicle and oocyte based on follicles and oocytes diameter respectively in a dose-dependent manner after six days of the antral follicle culturedhttps://jels.ub.ac.id/index.php/jels/article/view/397antral follicleflavonoidin vitrooocytewater clover. |
| spellingShingle | Siska Nanda Widhaningrum Septiawan Putranto Sri Rahayu Gatot Ciptadi Effect of Water Clover (Marsilea crenata) Ethanol Extracts on Follicle and Oocyte Diameter of Goat: In Vitro Study Journal of Experimental Life Science antral follicle flavonoid in vitro oocyte water clover. |
| title | Effect of Water Clover (Marsilea crenata) Ethanol Extracts on Follicle and Oocyte Diameter of Goat: In Vitro Study |
| title_full | Effect of Water Clover (Marsilea crenata) Ethanol Extracts on Follicle and Oocyte Diameter of Goat: In Vitro Study |
| title_fullStr | Effect of Water Clover (Marsilea crenata) Ethanol Extracts on Follicle and Oocyte Diameter of Goat: In Vitro Study |
| title_full_unstemmed | Effect of Water Clover (Marsilea crenata) Ethanol Extracts on Follicle and Oocyte Diameter of Goat: In Vitro Study |
| title_short | Effect of Water Clover (Marsilea crenata) Ethanol Extracts on Follicle and Oocyte Diameter of Goat: In Vitro Study |
| title_sort | effect of water clover marsilea crenata ethanol extracts on follicle and oocyte diameter of goat in vitro study |
| topic | antral follicle flavonoid in vitro oocyte water clover. |
| url | https://jels.ub.ac.id/index.php/jels/article/view/397 |
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