Study of Testicular Structure in Fetuses with Prune Belly Syndrome
Purpose. To compare the structure of the testis in fetuses with prune belly syndrome (PBS) to normal controls. Materials and Methods. We studied 6 testes obtained from 3 fetuses with PBS and 14 testes from 7 male fetuses. The testicular specimens were cut into 5-μm thick sections and stained with he...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Advances in Urology |
| Online Access: | http://dx.doi.org/10.1155/2017/3254980 |
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| author | Luciano A. Favorito Suelen F. Costa Waldemar S. Costa Rodrigo Vieiralves Fabio O. Bernardo Francisco J. B. Sampaio |
| author_facet | Luciano A. Favorito Suelen F. Costa Waldemar S. Costa Rodrigo Vieiralves Fabio O. Bernardo Francisco J. B. Sampaio |
| author_sort | Luciano A. Favorito |
| collection | DOAJ |
| description | Purpose. To compare the structure of the testis in fetuses with prune belly syndrome (PBS) to normal controls. Materials and Methods. We studied 6 testes obtained from 3 fetuses with PBS and 14 testes from 7 male fetuses. The testicular specimens were cut into 5-μm thick sections and stained with hematoxylin and eosin (HE), to observe the seminiferous tubules; Weigert’s solution to observe elastic fibers; and picrosirius red to observe collagen. The images were captured with an Olympus BX51 microscope and Olympus DP70 camera. The stereological analysis was done with the Image Pro and Image J programs. Means were statistically compared using the Mann–Whitney U test (p<0.005). Results. Quantitative analysis documented no differences (p=0.4) in number of seminiferous tubules (ST) in PBS testes (mean = 8.87%, SD=1.59), when compared to the control (mean = 11.4%, SD=2.99) and no differences (p=0.8) in diameter of ST in PBS testes (mean = 52.85 μm, SD=1.58) when compared to the control group (mean = 53.17 μm, SD=1.55), but we did observe a lower number (p=0.0002) of Leydig cells in the PBS testes (mean = 67.03% and SD=3.697) when compared to the control group (mean = 90.1% and SD=2.986). Conclusions. Our study showed a lower concentration of Leydig cells in the triad syndrome fetuses. |
| format | Article |
| id | doaj-art-ab7e514fd4bc4761a3d3e56d7d9c2677 |
| institution | OA Journals |
| issn | 1687-6369 1687-6377 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Urology |
| spelling | doaj-art-ab7e514fd4bc4761a3d3e56d7d9c26772025-08-20T02:20:13ZengWileyAdvances in Urology1687-63691687-63772017-01-01201710.1155/2017/32549803254980Study of Testicular Structure in Fetuses with Prune Belly SyndromeLuciano A. Favorito0Suelen F. Costa1Waldemar S. Costa2Rodrigo Vieiralves3Fabio O. Bernardo4Francisco J. B. Sampaio5Urogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, RJ, BrazilUrogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, RJ, BrazilUrogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, RJ, BrazilUrogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, RJ, BrazilUrogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, RJ, BrazilUrogenital Research Unit, State University of Rio de Janeiro, Rio de Janeiro, RJ, BrazilPurpose. To compare the structure of the testis in fetuses with prune belly syndrome (PBS) to normal controls. Materials and Methods. We studied 6 testes obtained from 3 fetuses with PBS and 14 testes from 7 male fetuses. The testicular specimens were cut into 5-μm thick sections and stained with hematoxylin and eosin (HE), to observe the seminiferous tubules; Weigert’s solution to observe elastic fibers; and picrosirius red to observe collagen. The images were captured with an Olympus BX51 microscope and Olympus DP70 camera. The stereological analysis was done with the Image Pro and Image J programs. Means were statistically compared using the Mann–Whitney U test (p<0.005). Results. Quantitative analysis documented no differences (p=0.4) in number of seminiferous tubules (ST) in PBS testes (mean = 8.87%, SD=1.59), when compared to the control (mean = 11.4%, SD=2.99) and no differences (p=0.8) in diameter of ST in PBS testes (mean = 52.85 μm, SD=1.58) when compared to the control group (mean = 53.17 μm, SD=1.55), but we did observe a lower number (p=0.0002) of Leydig cells in the PBS testes (mean = 67.03% and SD=3.697) when compared to the control group (mean = 90.1% and SD=2.986). Conclusions. Our study showed a lower concentration of Leydig cells in the triad syndrome fetuses.http://dx.doi.org/10.1155/2017/3254980 |
| spellingShingle | Luciano A. Favorito Suelen F. Costa Waldemar S. Costa Rodrigo Vieiralves Fabio O. Bernardo Francisco J. B. Sampaio Study of Testicular Structure in Fetuses with Prune Belly Syndrome Advances in Urology |
| title | Study of Testicular Structure in Fetuses with Prune Belly Syndrome |
| title_full | Study of Testicular Structure in Fetuses with Prune Belly Syndrome |
| title_fullStr | Study of Testicular Structure in Fetuses with Prune Belly Syndrome |
| title_full_unstemmed | Study of Testicular Structure in Fetuses with Prune Belly Syndrome |
| title_short | Study of Testicular Structure in Fetuses with Prune Belly Syndrome |
| title_sort | study of testicular structure in fetuses with prune belly syndrome |
| url | http://dx.doi.org/10.1155/2017/3254980 |
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