Autonomous ribosome biogenesis in vitro
Abstract Ribosome biogenesis is pivotal in the self-replication of life. In Escherichia coli, three ribosomal RNAs and 54 ribosomal proteins are synthesized and subjected to cooperative hierarchical assembly facilitated by numerous accessory factors. Realizing ribosome biogenesis in vitro is a criti...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-55853-7 |
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| author | Yuishin Kosaka Yumi Miyawaki Megumi Mori Shunsuke Aburaya Chisato Nishizawa Takeshi Chujo Tatsuya Niwa Takumi Miyazaki Takashi Sugita Mao Fukuyama Hideki Taguchi Kazuhito Tomizawa Kenji Sugase Mitsuyoshi Ueda Wataru Aoki |
| author_facet | Yuishin Kosaka Yumi Miyawaki Megumi Mori Shunsuke Aburaya Chisato Nishizawa Takeshi Chujo Tatsuya Niwa Takumi Miyazaki Takashi Sugita Mao Fukuyama Hideki Taguchi Kazuhito Tomizawa Kenji Sugase Mitsuyoshi Ueda Wataru Aoki |
| author_sort | Yuishin Kosaka |
| collection | DOAJ |
| description | Abstract Ribosome biogenesis is pivotal in the self-replication of life. In Escherichia coli, three ribosomal RNAs and 54 ribosomal proteins are synthesized and subjected to cooperative hierarchical assembly facilitated by numerous accessory factors. Realizing ribosome biogenesis in vitro is a critical milestone for understanding the self-replication of life and creating artificial cells. Despite its importance, this goal has not yet been achieved owing to its complexity. In this study, we report the successful realization of ribosome biogenesis in vitro. Specifically, we developed a highly specific and sensitive reporter assay for the detection of nascent ribosomes. The reporter assay allowed for combinatorial and iterative exploration of reaction conditions for ribosome biogenesis, leading to the simultaneous, autonomous synthesis of both small and large subunits of ribosomes in vitro through transcription, translation, processing, and assembly in a single reaction space. Our achievement represents a crucial advancement toward revealing the fundamental principles underlying the self-replication of life and creating artificial cells. |
| format | Article |
| id | doaj-art-ab724158d22e4ef1b3c9fec0c63fd669 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-ab724158d22e4ef1b3c9fec0c63fd6692025-01-12T12:31:00ZengNature PortfolioNature Communications2041-17232025-01-0116111410.1038/s41467-025-55853-7Autonomous ribosome biogenesis in vitroYuishin Kosaka0Yumi Miyawaki1Megumi Mori2Shunsuke Aburaya3Chisato Nishizawa4Takeshi Chujo5Tatsuya Niwa6Takumi Miyazaki7Takashi Sugita8Mao Fukuyama9Hideki Taguchi10Kazuhito Tomizawa11Kenji Sugase12Mitsuyoshi Ueda13Wataru Aoki14Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto UniversityDivision of Applied Life Sciences, Graduate School of Agriculture, Kyoto UniversityDivision of Applied Life Sciences, Graduate School of Agriculture, Kyoto UniversityDivision of Metabolomics, Medical Institute of Bioregulation, Kyushu UniversityDivision of Applied Life Sciences, Graduate School of Agriculture, Kyoto UniversityDepartment of Molecular Physiology, Faculty of Life Sciences, Kumamoto UniversityCell Biology Center, Institute of Integrated Research, Institute of Science TokyoDivision of Applied Life Sciences, Graduate School of Agriculture, Kyoto UniversityTechnoPro, Inc. TechnoPro R&D, CompanyJST FORESTCell Biology Center, Institute of Integrated Research, Institute of Science TokyoDepartment of Molecular Physiology, Faculty of Life Sciences, Kumamoto UniversityDivision of Applied Life Sciences, Graduate School of Agriculture, Kyoto UniversityDivision of Applied Life Sciences, Graduate School of Agriculture, Kyoto UniversityJST FORESTAbstract Ribosome biogenesis is pivotal in the self-replication of life. In Escherichia coli, three ribosomal RNAs and 54 ribosomal proteins are synthesized and subjected to cooperative hierarchical assembly facilitated by numerous accessory factors. Realizing ribosome biogenesis in vitro is a critical milestone for understanding the self-replication of life and creating artificial cells. Despite its importance, this goal has not yet been achieved owing to its complexity. In this study, we report the successful realization of ribosome biogenesis in vitro. Specifically, we developed a highly specific and sensitive reporter assay for the detection of nascent ribosomes. The reporter assay allowed for combinatorial and iterative exploration of reaction conditions for ribosome biogenesis, leading to the simultaneous, autonomous synthesis of both small and large subunits of ribosomes in vitro through transcription, translation, processing, and assembly in a single reaction space. Our achievement represents a crucial advancement toward revealing the fundamental principles underlying the self-replication of life and creating artificial cells.https://doi.org/10.1038/s41467-025-55853-7 |
| spellingShingle | Yuishin Kosaka Yumi Miyawaki Megumi Mori Shunsuke Aburaya Chisato Nishizawa Takeshi Chujo Tatsuya Niwa Takumi Miyazaki Takashi Sugita Mao Fukuyama Hideki Taguchi Kazuhito Tomizawa Kenji Sugase Mitsuyoshi Ueda Wataru Aoki Autonomous ribosome biogenesis in vitro Nature Communications |
| title | Autonomous ribosome biogenesis in vitro |
| title_full | Autonomous ribosome biogenesis in vitro |
| title_fullStr | Autonomous ribosome biogenesis in vitro |
| title_full_unstemmed | Autonomous ribosome biogenesis in vitro |
| title_short | Autonomous ribosome biogenesis in vitro |
| title_sort | autonomous ribosome biogenesis in vitro |
| url | https://doi.org/10.1038/s41467-025-55853-7 |
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