Abnormal expression of miR-330-3p predicts post-prostatectomy urinary incontinence and regulates the function of urethral fibroblasts by targeting MMP2
Abstract Background Post-prostatectomy urinary incontinence (PPUI) is a common complication for patients with prostate cancer after surgery. MicroRNA-330-3p (miR-330-3p) is down-regulated in stress urinary incontinence patients. However, its clinical role and regulatory mechanism in PPUI remain unkn...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-06-01
|
| Series: | Hereditas |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41065-025-00475-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850207448934121472 |
|---|---|
| author | Xiaoying Feng Yuanyuan Mi Mengye Weng |
| author_facet | Xiaoying Feng Yuanyuan Mi Mengye Weng |
| author_sort | Xiaoying Feng |
| collection | DOAJ |
| description | Abstract Background Post-prostatectomy urinary incontinence (PPUI) is a common complication for patients with prostate cancer after surgery. MicroRNA-330-3p (miR-330-3p) is down-regulated in stress urinary incontinence patients. However, its clinical role and regulatory mechanism in PPUI remain unknown. Objective To assess the clinical significance of miR-330-3p in PPUI and to explore the potential mechanisms via matrix metalloproteinase 2 (MMP2) regulation. Methods This study enrolled 135 ageing prostate cancer patients (86 without PPUI, 49 with PPUI). Reverse transcription PCR (RT-qPCR) was utilized to measure the levels of miR-330-3p, while Receiver operating characteristic (ROC) analysis was conducted to evaluate the predictive significance of miR-330-3p for PPUI. The proliferative of human urethral fibroblasts (HUFs) was assessed by Cell Counting Kit-8 (CCK-8) assay, while inflammatory cytokines were quantified via enzyme-linked immunosorbent assay (ELISA) kits. Western blot assay was employed to examine the protein levels of extracellular matrix (ECM) remodeling-related markers. The miR-330-3p/MMP2 interaction was validated by dual-luciferase assay. Result miR-330-3p was significantly downregulated in PPUI patients, with low expression predicting PPUI. In HUFs, miR-330-3p overexpression inhibited IL-1β-induced hyperproliferation, inflammation, and ECM degradation. Overexpression of MMP2 counteracted the influence of miR-330-3p mimic on HUFs. Conclusion miR-330-3p is a potential biomarker for PPUI and regulates the function of urethral fibroblasts by targeting MMP2. |
| format | Article |
| id | doaj-art-ab71f0e75c5647e8b0e275d2a92a8063 |
| institution | OA Journals |
| issn | 1601-5223 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Hereditas |
| spelling | doaj-art-ab71f0e75c5647e8b0e275d2a92a80632025-08-20T02:10:31ZengBMCHereditas1601-52232025-06-01162111110.1186/s41065-025-00475-8Abnormal expression of miR-330-3p predicts post-prostatectomy urinary incontinence and regulates the function of urethral fibroblasts by targeting MMP2Xiaoying Feng0Yuanyuan Mi1Mengye Weng2Department of Urology Surgery, Affiliated Hospital of Jiangnan UniversityDepartment of Urology Surgery, Affiliated Hospital of Jiangnan UniversityDepartment of Orthopedics, Affiliated Hospital of Jiangnan UniversityAbstract Background Post-prostatectomy urinary incontinence (PPUI) is a common complication for patients with prostate cancer after surgery. MicroRNA-330-3p (miR-330-3p) is down-regulated in stress urinary incontinence patients. However, its clinical role and regulatory mechanism in PPUI remain unknown. Objective To assess the clinical significance of miR-330-3p in PPUI and to explore the potential mechanisms via matrix metalloproteinase 2 (MMP2) regulation. Methods This study enrolled 135 ageing prostate cancer patients (86 without PPUI, 49 with PPUI). Reverse transcription PCR (RT-qPCR) was utilized to measure the levels of miR-330-3p, while Receiver operating characteristic (ROC) analysis was conducted to evaluate the predictive significance of miR-330-3p for PPUI. The proliferative of human urethral fibroblasts (HUFs) was assessed by Cell Counting Kit-8 (CCK-8) assay, while inflammatory cytokines were quantified via enzyme-linked immunosorbent assay (ELISA) kits. Western blot assay was employed to examine the protein levels of extracellular matrix (ECM) remodeling-related markers. The miR-330-3p/MMP2 interaction was validated by dual-luciferase assay. Result miR-330-3p was significantly downregulated in PPUI patients, with low expression predicting PPUI. In HUFs, miR-330-3p overexpression inhibited IL-1β-induced hyperproliferation, inflammation, and ECM degradation. Overexpression of MMP2 counteracted the influence of miR-330-3p mimic on HUFs. Conclusion miR-330-3p is a potential biomarker for PPUI and regulates the function of urethral fibroblasts by targeting MMP2.https://doi.org/10.1186/s41065-025-00475-8miR-330-3pMMP2Post-prostatectomy urinary incontinenceInflammationECM remodeling |
| spellingShingle | Xiaoying Feng Yuanyuan Mi Mengye Weng Abnormal expression of miR-330-3p predicts post-prostatectomy urinary incontinence and regulates the function of urethral fibroblasts by targeting MMP2 Hereditas miR-330-3p MMP2 Post-prostatectomy urinary incontinence Inflammation ECM remodeling |
| title | Abnormal expression of miR-330-3p predicts post-prostatectomy urinary incontinence and regulates the function of urethral fibroblasts by targeting MMP2 |
| title_full | Abnormal expression of miR-330-3p predicts post-prostatectomy urinary incontinence and regulates the function of urethral fibroblasts by targeting MMP2 |
| title_fullStr | Abnormal expression of miR-330-3p predicts post-prostatectomy urinary incontinence and regulates the function of urethral fibroblasts by targeting MMP2 |
| title_full_unstemmed | Abnormal expression of miR-330-3p predicts post-prostatectomy urinary incontinence and regulates the function of urethral fibroblasts by targeting MMP2 |
| title_short | Abnormal expression of miR-330-3p predicts post-prostatectomy urinary incontinence and regulates the function of urethral fibroblasts by targeting MMP2 |
| title_sort | abnormal expression of mir 330 3p predicts post prostatectomy urinary incontinence and regulates the function of urethral fibroblasts by targeting mmp2 |
| topic | miR-330-3p MMP2 Post-prostatectomy urinary incontinence Inflammation ECM remodeling |
| url | https://doi.org/10.1186/s41065-025-00475-8 |
| work_keys_str_mv | AT xiaoyingfeng abnormalexpressionofmir3303ppredictspostprostatectomyurinaryincontinenceandregulatesthefunctionofurethralfibroblastsbytargetingmmp2 AT yuanyuanmi abnormalexpressionofmir3303ppredictspostprostatectomyurinaryincontinenceandregulatesthefunctionofurethralfibroblastsbytargetingmmp2 AT mengyeweng abnormalexpressionofmir3303ppredictspostprostatectomyurinaryincontinenceandregulatesthefunctionofurethralfibroblastsbytargetingmmp2 |