Dynamic Mitotic Localization of the Centrosomal Kinases CDK1, Plk, AurK, and Nek2 in <i>Dictyostelium amoebae</i>
The centrosome of the amoebozoan model <i>Dictyostelium discoideum</i> provides the best-established model for an acentriolar centrosome outside the <i>Opisthokonta</i>. <i>Dictyostelium</i> exhibits an unusual centrosome cycle, in which duplication is initiated o...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-09-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/13/18/1513 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850261340180971520 |
|---|---|
| author | Stefan Krüger Nathalie Pfaff Ralph Gräf Irene Meyer |
| author_facet | Stefan Krüger Nathalie Pfaff Ralph Gräf Irene Meyer |
| author_sort | Stefan Krüger |
| collection | DOAJ |
| description | The centrosome of the amoebozoan model <i>Dictyostelium discoideum</i> provides the best-established model for an acentriolar centrosome outside the <i>Opisthokonta</i>. <i>Dictyostelium</i> exhibits an unusual centrosome cycle, in which duplication is initiated only at the G2/M transition and occurs entirely during the M phase. Little is known about the role of conserved centrosomal kinases in this process. Therefore, we have generated knock-in strains for Aurora (AurK), CDK1, cyclin B, Nek2, and Plk, replacing the endogenous genes with constructs expressing the respective green fluorescent Neon fusion proteins, driven by the endogenous promoters, and studied their behavior in living cells. Our results show that CDK1 and cyclin B arrive at the centrosome first, already during G2, followed by Plk, Nek2, and AurK. Furthermore, CDK1/cyclin B and AurK were dynamically localized at kinetochores, and AurK in addition at nucleoli. The putative roles of all four kinases in centrosome duplication, mitosis, cytokinesis, and nucleolar dynamics are discussed. |
| format | Article |
| id | doaj-art-ab5e33371988425da3b7ec98b786e2fc |
| institution | OA Journals |
| issn | 2073-4409 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj-art-ab5e33371988425da3b7ec98b786e2fc2025-08-20T01:55:27ZengMDPI AGCells2073-44092024-09-011318151310.3390/cells13181513Dynamic Mitotic Localization of the Centrosomal Kinases CDK1, Plk, AurK, and Nek2 in <i>Dictyostelium amoebae</i>Stefan Krüger0Nathalie Pfaff1Ralph Gräf2Irene Meyer3Department of Cell Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam-Golm, GermanyDepartment of Cell Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam-Golm, GermanyDepartment of Cell Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam-Golm, GermanyDepartment of Cell Biology, University of Potsdam, Karl-Liebknecht-Str. 24-25, 14476 Potsdam-Golm, GermanyThe centrosome of the amoebozoan model <i>Dictyostelium discoideum</i> provides the best-established model for an acentriolar centrosome outside the <i>Opisthokonta</i>. <i>Dictyostelium</i> exhibits an unusual centrosome cycle, in which duplication is initiated only at the G2/M transition and occurs entirely during the M phase. Little is known about the role of conserved centrosomal kinases in this process. Therefore, we have generated knock-in strains for Aurora (AurK), CDK1, cyclin B, Nek2, and Plk, replacing the endogenous genes with constructs expressing the respective green fluorescent Neon fusion proteins, driven by the endogenous promoters, and studied their behavior in living cells. Our results show that CDK1 and cyclin B arrive at the centrosome first, already during G2, followed by Plk, Nek2, and AurK. Furthermore, CDK1/cyclin B and AurK were dynamically localized at kinetochores, and AurK in addition at nucleoli. The putative roles of all four kinases in centrosome duplication, mitosis, cytokinesis, and nucleolar dynamics are discussed.https://www.mdpi.com/2073-4409/13/18/1513kinasemitosiscentrosome<i>Dictyostelium</i>CDK1cyclin |
| spellingShingle | Stefan Krüger Nathalie Pfaff Ralph Gräf Irene Meyer Dynamic Mitotic Localization of the Centrosomal Kinases CDK1, Plk, AurK, and Nek2 in <i>Dictyostelium amoebae</i> Cells kinase mitosis centrosome <i>Dictyostelium</i> CDK1 cyclin |
| title | Dynamic Mitotic Localization of the Centrosomal Kinases CDK1, Plk, AurK, and Nek2 in <i>Dictyostelium amoebae</i> |
| title_full | Dynamic Mitotic Localization of the Centrosomal Kinases CDK1, Plk, AurK, and Nek2 in <i>Dictyostelium amoebae</i> |
| title_fullStr | Dynamic Mitotic Localization of the Centrosomal Kinases CDK1, Plk, AurK, and Nek2 in <i>Dictyostelium amoebae</i> |
| title_full_unstemmed | Dynamic Mitotic Localization of the Centrosomal Kinases CDK1, Plk, AurK, and Nek2 in <i>Dictyostelium amoebae</i> |
| title_short | Dynamic Mitotic Localization of the Centrosomal Kinases CDK1, Plk, AurK, and Nek2 in <i>Dictyostelium amoebae</i> |
| title_sort | dynamic mitotic localization of the centrosomal kinases cdk1 plk aurk and nek2 in i dictyostelium amoebae i |
| topic | kinase mitosis centrosome <i>Dictyostelium</i> CDK1 cyclin |
| url | https://www.mdpi.com/2073-4409/13/18/1513 |
| work_keys_str_mv | AT stefankruger dynamicmitoticlocalizationofthecentrosomalkinasescdk1plkaurkandnek2inidictyosteliumamoebaei AT nathaliepfaff dynamicmitoticlocalizationofthecentrosomalkinasescdk1plkaurkandnek2inidictyosteliumamoebaei AT ralphgraf dynamicmitoticlocalizationofthecentrosomalkinasescdk1plkaurkandnek2inidictyosteliumamoebaei AT irenemeyer dynamicmitoticlocalizationofthecentrosomalkinasescdk1plkaurkandnek2inidictyosteliumamoebaei |