Protocol for click labeling of HIV-1 envelope on amber-free virions prepared using genetic code expansion

Protocol for click labeling of HIV-1 envelope on amber-free virions prepared using genetic code expansion

Summary: Site-specific fluorescent labeling of HIV-1 proteins enables direct visualization of their dynamics and trafficking. Here, we present a protocol to label HIV-1 envelope (Env) glycoproteins on virions using genetic code expansion and click chemistry. We describe steps for using HEK293T cells...

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Bibliographic Details
Main Authors: Wang Xu, Narendra Kumar Gonepudi, Maolin Lu
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Cell-based Assays
Microbiology
Chemistry
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725004010
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