Novel Halotolerant Bacteria from Saline Environments: Isolation and Biomolecule Production
Microorganisms from saline environments have garnered significant interest due to their unique adaptations, which enable them to thrive under high-salt conditions and synthesize valuable biomolecules. This study investigates the biosynthesis of biomolecules, such as extracellular hydrolytic enzymes,...
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2025-06-01
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| author | Simona Neagu Mihaela Marilena Stancu |
| author_facet | Simona Neagu Mihaela Marilena Stancu |
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| description | Microorganisms from saline environments have garnered significant interest due to their unique adaptations, which enable them to thrive under high-salt conditions and synthesize valuable biomolecules. This study investigates the biosynthesis of biomolecules, such as extracellular hydrolytic enzymes, biosurfactants, and carotenoid pigments, by four newly halotolerant bacterial strains isolated from saline environments in the Băicoi (soil, water) and Curmătura (mud) area (Prahova County, Romania). Isolation was performed on two selective culture media with different NaCl concentrations (1.7 M, 3.4 M). Based on their phenotypic and molecular characteristics, the four halotolerant bacteria were identified as <i>Halomonas elongata</i> SB8, <i>Bacillus altitudinis</i> CN6, <i>Planococcus rifietoensis</i> CN8, and <i>Halomonas stenophila</i> IB5. The two bacterial strains from the <i>Halomonas</i> genus exhibited growth in MH medium containing elevated NaCl concentrations (0–5 M), in contrast to the other two strains from <i>Bacillus</i> (0–2 M) and <i>Planococcus</i> (0–3 M). The growth of these bacteria under different salinity conditions, hydrocarbon tolerance, and biomolecule production were assessed through biochemical assays, spectrophotometry, and high-performance thin-layer chromatography. The antimicrobial properties of biosurfactants and carotenoids produced by <i>H. elongata</i> SB8, <i>B. altitudinis</i> CN6, <i>P. rifietoensis</i> CN8, and <i>H. stenophila</i> IB5 were evaluated against four reference pathogenic microorganisms from the genera <i>Escherichia</i>, <i>Pseudomonas</i>, <i>Staphylococcus</i>, and <i>Candida</i>. <i>H. elongata</i> SB8 showed the highest hydrocarbon tolerance. <i>B. altitudinis</i> CN6 exhibited multiple hydrolase activities and, along with <i>H. elongata</i> SB8, demonstrated biosurfactant production. <i>P. rifietoensis</i> CN8 produced the highest carotenoid concentration with antifungal and antimicrobial activity. Exploring these organisms opens new pathways for bioremediation, industrial bioprocessing, and sustainable biomolecule production. |
| format | Article |
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| institution | OA Journals |
| issn | 2673-6284 |
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| publishDate | 2025-06-01 |
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| spelling | doaj-art-ab3fa7efebe04df88bcbc75262b89bd92025-08-20T02:24:23ZengMDPI AGBioTech2673-62842025-06-011424910.3390/biotech14020049Novel Halotolerant Bacteria from Saline Environments: Isolation and Biomolecule ProductionSimona Neagu0Mihaela Marilena Stancu1Institute of Biology Bucharest of Romanian Academy, 296 Splaiul Independentei, 060031 Bucharest, RomaniaInstitute of Biology Bucharest of Romanian Academy, 296 Splaiul Independentei, 060031 Bucharest, RomaniaMicroorganisms from saline environments have garnered significant interest due to their unique adaptations, which enable them to thrive under high-salt conditions and synthesize valuable biomolecules. This study investigates the biosynthesis of biomolecules, such as extracellular hydrolytic enzymes, biosurfactants, and carotenoid pigments, by four newly halotolerant bacterial strains isolated from saline environments in the Băicoi (soil, water) and Curmătura (mud) area (Prahova County, Romania). Isolation was performed on two selective culture media with different NaCl concentrations (1.7 M, 3.4 M). Based on their phenotypic and molecular characteristics, the four halotolerant bacteria were identified as <i>Halomonas elongata</i> SB8, <i>Bacillus altitudinis</i> CN6, <i>Planococcus rifietoensis</i> CN8, and <i>Halomonas stenophila</i> IB5. The two bacterial strains from the <i>Halomonas</i> genus exhibited growth in MH medium containing elevated NaCl concentrations (0–5 M), in contrast to the other two strains from <i>Bacillus</i> (0–2 M) and <i>Planococcus</i> (0–3 M). The growth of these bacteria under different salinity conditions, hydrocarbon tolerance, and biomolecule production were assessed through biochemical assays, spectrophotometry, and high-performance thin-layer chromatography. The antimicrobial properties of biosurfactants and carotenoids produced by <i>H. elongata</i> SB8, <i>B. altitudinis</i> CN6, <i>P. rifietoensis</i> CN8, and <i>H. stenophila</i> IB5 were evaluated against four reference pathogenic microorganisms from the genera <i>Escherichia</i>, <i>Pseudomonas</i>, <i>Staphylococcus</i>, and <i>Candida</i>. <i>H. elongata</i> SB8 showed the highest hydrocarbon tolerance. <i>B. altitudinis</i> CN6 exhibited multiple hydrolase activities and, along with <i>H. elongata</i> SB8, demonstrated biosurfactant production. <i>P. rifietoensis</i> CN8 produced the highest carotenoid concentration with antifungal and antimicrobial activity. Exploring these organisms opens new pathways for bioremediation, industrial bioprocessing, and sustainable biomolecule production.https://www.mdpi.com/2673-6284/14/2/49saline environmentshalotolerant bacteriabiomoleculesantimicrobial activity |
| spellingShingle | Simona Neagu Mihaela Marilena Stancu Novel Halotolerant Bacteria from Saline Environments: Isolation and Biomolecule Production BioTech saline environments halotolerant bacteria biomolecules antimicrobial activity |
| title | Novel Halotolerant Bacteria from Saline Environments: Isolation and Biomolecule Production |
| title_full | Novel Halotolerant Bacteria from Saline Environments: Isolation and Biomolecule Production |
| title_fullStr | Novel Halotolerant Bacteria from Saline Environments: Isolation and Biomolecule Production |
| title_full_unstemmed | Novel Halotolerant Bacteria from Saline Environments: Isolation and Biomolecule Production |
| title_short | Novel Halotolerant Bacteria from Saline Environments: Isolation and Biomolecule Production |
| title_sort | novel halotolerant bacteria from saline environments isolation and biomolecule production |
| topic | saline environments halotolerant bacteria biomolecules antimicrobial activity |
| url | https://www.mdpi.com/2673-6284/14/2/49 |
| work_keys_str_mv | AT simonaneagu novelhalotolerantbacteriafromsalineenvironmentsisolationandbiomoleculeproduction AT mihaelamarilenastancu novelhalotolerantbacteriafromsalineenvironmentsisolationandbiomoleculeproduction |