Dataset of metazoan identification using 18s rRNA eDNA metabarcoding from seawater at Hybrid-Ocean Thermal Energy Conversion (H-OTEC) site of Peninsular MalaysiaNational Center for Biotechnology Information
Environmental DNA (eDNA) is a very useful method for studying the biodiversity as it obtains genetic materials from environmental samples such as soil, water and air. This highly sensitive detection could attain information despite the scarcity of population densities. This non-invasive approach is...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-04-01
|
| Series: | Data in Brief |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340925001337 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849773138050547712 |
|---|---|
| author | Herryawan Ryadi Eziwar Dyari Muhammad Adam Muhammad Rasib Jaafar Abu Bakar Su Nyun Pau Suriyanti |
| author_facet | Herryawan Ryadi Eziwar Dyari Muhammad Adam Muhammad Rasib Jaafar Abu Bakar Su Nyun Pau Suriyanti |
| author_sort | Herryawan Ryadi Eziwar Dyari |
| collection | DOAJ |
| description | Environmental DNA (eDNA) is a very useful method for studying the biodiversity as it obtains genetic materials from environmental samples such as soil, water and air. This highly sensitive detection could attain information despite the scarcity of population densities. This non-invasive approach is feasible particularly in impact assessment of marine development. Hybrid Ocean Thermal Energy Conversion (H-OTEC) is a renewable energy technology that generates electricity by using sea water temperature differentials. While the H-OTEC is currently in its pre-commissioning state, it is crucial to provide a baseline data on the marine biota in this site. This study targets the 18s rRNA genes of the marine metazoans at the H-OTEC plant site at Port Dickson, Negeri Sembilan, Malaysia. Ten litres of seawater were collected from the site and immediately filtered through a 0.2 µm cellulose membrane filter. The eDNA extraction was carried out using cetyltrimethylammonium bromide (CTAB) protocol and sent for next generation sequencing. High-throughput sequencing using the Illumina platform produced 113,980 filtered reads, corresponding to 73 genera, 64 families, and 6 phyla of marine metazoans in the area under examination. The highest relative abundance belongs to the phylum Arthropoda (85%), family Paracalanidae (45%) and genus Bestiolina (38%). The diversity indices indicate a great deal of biodiversity in the ecosystem. |
| format | Article |
| id | doaj-art-ab3d71c887c340f1851405cc3a8d30e0 |
| institution | DOAJ |
| issn | 2352-3409 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj-art-ab3d71c887c340f1851405cc3a8d30e02025-08-20T03:02:07ZengElsevierData in Brief2352-34092025-04-015911140110.1016/j.dib.2025.111401Dataset of metazoan identification using 18s rRNA eDNA metabarcoding from seawater at Hybrid-Ocean Thermal Energy Conversion (H-OTEC) site of Peninsular MalaysiaNational Center for Biotechnology InformationHerryawan Ryadi Eziwar Dyari0Muhammad Adam Muhammad Rasib1Jaafar Abu Bakar2Su Nyun Pau Suriyanti3Department of Earth Science and Environment, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia; Marine Ecosystem Research Centre, The Centre for Natural and Physical Laboratory Management, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia; Department of Aquaculture, Fisheries and Marine Faculty, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, East Java, IndonesiaDepartment of Earth Science and Environment, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, MalaysiaOcean Thermal Energy Centre (OTEC), Institute of Future Energy, Universiti Teknologi Malaysia, Block Q, Jalan Sultan Yahya Petra, 54100 Kuala Lumpur, MalaysiaDepartment of Earth Science and Environment, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia; Marine Ecosystem Research Centre, The Centre for Natural and Physical Laboratory Management, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia; Department of Aquaculture, Fisheries and Marine Faculty, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, East Java, Indonesia; Corresponding author.Environmental DNA (eDNA) is a very useful method for studying the biodiversity as it obtains genetic materials from environmental samples such as soil, water and air. This highly sensitive detection could attain information despite the scarcity of population densities. This non-invasive approach is feasible particularly in impact assessment of marine development. Hybrid Ocean Thermal Energy Conversion (H-OTEC) is a renewable energy technology that generates electricity by using sea water temperature differentials. While the H-OTEC is currently in its pre-commissioning state, it is crucial to provide a baseline data on the marine biota in this site. This study targets the 18s rRNA genes of the marine metazoans at the H-OTEC plant site at Port Dickson, Negeri Sembilan, Malaysia. Ten litres of seawater were collected from the site and immediately filtered through a 0.2 µm cellulose membrane filter. The eDNA extraction was carried out using cetyltrimethylammonium bromide (CTAB) protocol and sent for next generation sequencing. High-throughput sequencing using the Illumina platform produced 113,980 filtered reads, corresponding to 73 genera, 64 families, and 6 phyla of marine metazoans in the area under examination. The highest relative abundance belongs to the phylum Arthropoda (85%), family Paracalanidae (45%) and genus Bestiolina (38%). The diversity indices indicate a great deal of biodiversity in the ecosystem.http://www.sciencedirect.com/science/article/pii/S2352340925001337Marine biodiversityPort DicksonRenewable energySustainable development goalsLife below water |
| spellingShingle | Herryawan Ryadi Eziwar Dyari Muhammad Adam Muhammad Rasib Jaafar Abu Bakar Su Nyun Pau Suriyanti Dataset of metazoan identification using 18s rRNA eDNA metabarcoding from seawater at Hybrid-Ocean Thermal Energy Conversion (H-OTEC) site of Peninsular MalaysiaNational Center for Biotechnology Information Data in Brief Marine biodiversity Port Dickson Renewable energy Sustainable development goals Life below water |
| title | Dataset of metazoan identification using 18s rRNA eDNA metabarcoding from seawater at Hybrid-Ocean Thermal Energy Conversion (H-OTEC) site of Peninsular MalaysiaNational Center for Biotechnology Information |
| title_full | Dataset of metazoan identification using 18s rRNA eDNA metabarcoding from seawater at Hybrid-Ocean Thermal Energy Conversion (H-OTEC) site of Peninsular MalaysiaNational Center for Biotechnology Information |
| title_fullStr | Dataset of metazoan identification using 18s rRNA eDNA metabarcoding from seawater at Hybrid-Ocean Thermal Energy Conversion (H-OTEC) site of Peninsular MalaysiaNational Center for Biotechnology Information |
| title_full_unstemmed | Dataset of metazoan identification using 18s rRNA eDNA metabarcoding from seawater at Hybrid-Ocean Thermal Energy Conversion (H-OTEC) site of Peninsular MalaysiaNational Center for Biotechnology Information |
| title_short | Dataset of metazoan identification using 18s rRNA eDNA metabarcoding from seawater at Hybrid-Ocean Thermal Energy Conversion (H-OTEC) site of Peninsular MalaysiaNational Center for Biotechnology Information |
| title_sort | dataset of metazoan identification using 18s rrna edna metabarcoding from seawater at hybrid ocean thermal energy conversion h otec site of peninsular malaysianational center for biotechnology information |
| topic | Marine biodiversity Port Dickson Renewable energy Sustainable development goals Life below water |
| url | http://www.sciencedirect.com/science/article/pii/S2352340925001337 |
| work_keys_str_mv | AT herryawanryadieziwardyari datasetofmetazoanidentificationusing18srrnaednametabarcodingfromseawaterathybridoceanthermalenergyconversionhotecsiteofpeninsularmalaysianationalcenterforbiotechnologyinformation AT muhammadadammuhammadrasib datasetofmetazoanidentificationusing18srrnaednametabarcodingfromseawaterathybridoceanthermalenergyconversionhotecsiteofpeninsularmalaysianationalcenterforbiotechnologyinformation AT jaafarabubakar datasetofmetazoanidentificationusing18srrnaednametabarcodingfromseawaterathybridoceanthermalenergyconversionhotecsiteofpeninsularmalaysianationalcenterforbiotechnologyinformation AT sunyunpausuriyanti datasetofmetazoanidentificationusing18srrnaednametabarcodingfromseawaterathybridoceanthermalenergyconversionhotecsiteofpeninsularmalaysianationalcenterforbiotechnologyinformation |