Ecological dynamics and microbial community composition of Heliotropium arboreum in the coastal ecosystems of Hainan province

Heliotropium arboreum, a resilient plant species found in the coastal ecosystems of Hainan Province, thrives in nutrient-poor, isolated habitats. This study examines the microbial community composition and ecological dynamics of H. arboreum across several islands in the South China Sea, aiming to un...

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Main Authors: Xiaofeng Zhang, Fazhi Fang, Linhua Sha, Mir Muhammad Nizamani
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1611262/full
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author Xiaofeng Zhang
Xiaofeng Zhang
Xiaofeng Zhang
Fazhi Fang
Fazhi Fang
Fazhi Fang
Linhua Sha
Linhua Sha
Linhua Sha
Mir Muhammad Nizamani
author_facet Xiaofeng Zhang
Xiaofeng Zhang
Xiaofeng Zhang
Fazhi Fang
Fazhi Fang
Fazhi Fang
Linhua Sha
Linhua Sha
Linhua Sha
Mir Muhammad Nizamani
author_sort Xiaofeng Zhang
collection DOAJ
description Heliotropium arboreum, a resilient plant species found in the coastal ecosystems of Hainan Province, thrives in nutrient-poor, isolated habitats. This study examines the microbial community composition and ecological dynamics of H. arboreum across several islands in the South China Sea, aiming to understand how environmental factors like soil pH, nitrogen (N), and phosphorus (P) availability influence the plant's microbial communities and ecological success. The study was conducted across seven plots located on different islands, including Zhaoshu Island, Yongxing Island, and Wuzhizhou Island, among others. Soil samples were collected from three layers: 0–10 cm, 10–20 cm, and 20–30 cm. A total of 63 soil samples were processed for physicochemical analysis, measuring pH, organic matter, total nitrogen (TN), total phosphorus (TP), available phosphorus (AP), available potassium (AK), ammonium nitrogen (NH4+), and nitrate nitrogen (NO3-). Microbial DNA was extracted, amplified, and sequenced using Illumina-based platforms, producing 5,272,525 high-quality bacterial sequences and 5,273,089 fungal sequences. Bacterial communities were primarily composed of Proteobacteria (range: 29–50%), Planctomycetota, and Acidobacteriota, with significant correlations between certain bacterial genera and nutrient availability. Notably, Bryobacter (r = 0.810***) and Stenotrophobacter (r = 0.496***) showed strong positive correlations with nitrogen availability. Fungal communities were dominated by Ascomycota, followed by Basidiomycota. Preussia (r = 0.585***) and Metacordyceps (r = 0.616***) were positively correlated with nutrient availability. Diversity indices indicated that bacterial diversity was highest in pristine environments such as Zhaoshu Island (350 species), while fungal diversity was lower, with a maximum of 130 fungal species in less disturbed plots. The findings suggest that nutrient availability, particularly nitrogen and phosphorus, significantly shapes the microbial communities associated with H. arboreum. Bacterial diversity was positively correlated with nutrient-rich environments, while fungal diversity, especially Ascomycota, was more sensitive to disturbances, particularly in areas like Wuzhizhou Island and Yongxing Island, where human activity is higher. These results highlight the importance of maintaining nutrient availability and reducing human interference to preserve microbial diversity and the ecological resilience of H. arboreum in coastal ecosystems.
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spelling doaj-art-ab39fab05ce447b180fd29bccfc8bdd92025-08-20T02:38:46ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.16112621611262Ecological dynamics and microbial community composition of Heliotropium arboreum in the coastal ecosystems of Hainan provinceXiaofeng Zhang0Xiaofeng Zhang1Xiaofeng Zhang2Fazhi Fang3Fazhi Fang4Fazhi Fang5Linhua Sha6Linhua Sha7Linhua Sha8Mir Muhammad Nizamani9Hainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, ChinaKey Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, ChinaInnovation Platform for Academicians of Hainan Province, Haikou, ChinaHainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, ChinaKey Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, ChinaInnovation Platform for Academicians of Hainan Province, Haikou, ChinaHainan Academy of Forestry (Hainan Academy of Mangrove), Haikou, ChinaKey Laboratory of Tropical Forestry Resources Monitoring and Application of Hainan Province, Haikou, ChinaInnovation Platform for Academicians of Hainan Province, Haikou, ChinaInstitute of Marine Sciences, Guangdong Provincial Key Laboratory of Marine Disaster Prediction and Prevention, Shantou University, Shantou, ChinaHeliotropium arboreum, a resilient plant species found in the coastal ecosystems of Hainan Province, thrives in nutrient-poor, isolated habitats. This study examines the microbial community composition and ecological dynamics of H. arboreum across several islands in the South China Sea, aiming to understand how environmental factors like soil pH, nitrogen (N), and phosphorus (P) availability influence the plant's microbial communities and ecological success. The study was conducted across seven plots located on different islands, including Zhaoshu Island, Yongxing Island, and Wuzhizhou Island, among others. Soil samples were collected from three layers: 0–10 cm, 10–20 cm, and 20–30 cm. A total of 63 soil samples were processed for physicochemical analysis, measuring pH, organic matter, total nitrogen (TN), total phosphorus (TP), available phosphorus (AP), available potassium (AK), ammonium nitrogen (NH4+), and nitrate nitrogen (NO3-). Microbial DNA was extracted, amplified, and sequenced using Illumina-based platforms, producing 5,272,525 high-quality bacterial sequences and 5,273,089 fungal sequences. Bacterial communities were primarily composed of Proteobacteria (range: 29–50%), Planctomycetota, and Acidobacteriota, with significant correlations between certain bacterial genera and nutrient availability. Notably, Bryobacter (r = 0.810***) and Stenotrophobacter (r = 0.496***) showed strong positive correlations with nitrogen availability. Fungal communities were dominated by Ascomycota, followed by Basidiomycota. Preussia (r = 0.585***) and Metacordyceps (r = 0.616***) were positively correlated with nutrient availability. Diversity indices indicated that bacterial diversity was highest in pristine environments such as Zhaoshu Island (350 species), while fungal diversity was lower, with a maximum of 130 fungal species in less disturbed plots. The findings suggest that nutrient availability, particularly nitrogen and phosphorus, significantly shapes the microbial communities associated with H. arboreum. Bacterial diversity was positively correlated with nutrient-rich environments, while fungal diversity, especially Ascomycota, was more sensitive to disturbances, particularly in areas like Wuzhizhou Island and Yongxing Island, where human activity is higher. These results highlight the importance of maintaining nutrient availability and reducing human interference to preserve microbial diversity and the ecological resilience of H. arboreum in coastal ecosystems.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1611262/fullmicrobial diversitybacterial communitiesfungal communitiesenvironmental factorsSouth China Sea
spellingShingle Xiaofeng Zhang
Xiaofeng Zhang
Xiaofeng Zhang
Fazhi Fang
Fazhi Fang
Fazhi Fang
Linhua Sha
Linhua Sha
Linhua Sha
Mir Muhammad Nizamani
Ecological dynamics and microbial community composition of Heliotropium arboreum in the coastal ecosystems of Hainan province
Frontiers in Microbiology
microbial diversity
bacterial communities
fungal communities
environmental factors
South China Sea
title Ecological dynamics and microbial community composition of Heliotropium arboreum in the coastal ecosystems of Hainan province
title_full Ecological dynamics and microbial community composition of Heliotropium arboreum in the coastal ecosystems of Hainan province
title_fullStr Ecological dynamics and microbial community composition of Heliotropium arboreum in the coastal ecosystems of Hainan province
title_full_unstemmed Ecological dynamics and microbial community composition of Heliotropium arboreum in the coastal ecosystems of Hainan province
title_short Ecological dynamics and microbial community composition of Heliotropium arboreum in the coastal ecosystems of Hainan province
title_sort ecological dynamics and microbial community composition of heliotropium arboreum in the coastal ecosystems of hainan province
topic microbial diversity
bacterial communities
fungal communities
environmental factors
South China Sea
url https://www.frontiersin.org/articles/10.3389/fmicb.2025.1611262/full
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