Distribution of TEM, SHV, OXA AND CTX-M Genes among ESBL- producing E. coli Isolates from Patients with Urinary Tract Infections.
The emergence of extended-spectrum beta-lactamases (ESBLs) are as a result of transmission among Gram negative bacteria, usually by horizontal gene transfer. The epidemiology of ESBL genes is changing rapidly and shows marked geographic differences in distribution of genotypes. In this study, isolat...
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Hammer Head Production Limited
2020-03-01
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| Series: | Sokoto Journal of Medical Laboratory Science |
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| Online Access: | https://sokjmls.com.ng/index.php/SJMLS/article/view/146 |
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| author | Olley Mitsan , Maureen U. Okwu , Osakue Eguagie Osareniro , Alao James |
| author_facet | Olley Mitsan , Maureen U. Okwu , Osakue Eguagie Osareniro , Alao James |
| author_sort | Olley Mitsan , Maureen U. Okwu , Osakue Eguagie Osareniro , Alao James |
| collection | DOAJ |
| description | The emergence of extended-spectrum beta-lactamases (ESBLs) are as a result of transmission among Gram negative bacteria, usually by horizontal gene transfer. The epidemiology of ESBL genes is changing rapidly and shows marked geographic differences in distribution of genotypes. In this study, isolation of extended- spectrum beta- lactamase producing E. coli in patients with bacteriuria in parts of Edo state, Nigeria was carried out using the urine of patients with UTI in selected teaching hospitals namely : University of Benin Teaching Hospital (UBTH), Irrua Specialist Teaching Hospital, Irrua (ISTH) and Igbinedion University Teaching Hospital, Okada
(IUTH). A total of 3,378 urine samples were cultured into Blood agar, Cysteine lactose electrolyte deficient (CLED) agar and MacConkey agar and incubated aerobically at 37°C. Isolated E. coli had resistance of 38.5%, 43.9%, 36.4%, 38.5% and 52% to cefuroxime, ceftazidime, cefixime, ceftatriaxone and cefotaxime respectively. Phenotypic detection of ESBL producing E. coli was done using the double disk synergism. ESBL- producing E. coli had prevalence of 1.5%, genomic DNA extraction was carried out on the E. coli that were ESBL positive and hybridization effected using specific primers for the assayed genes. The BlaTEM, BlaCTX, BlaOXA and BlaSHV genes had prevalence of 33.3%, 53.3%, 6.7% and 6.7% respectively. This study has established the presence of these resistant genes among the E. coli isolates from patients with urinary tract infections. |
| format | Article |
| id | doaj-art-ab32ab092fc6485bb701d190ed52bcde |
| institution | DOAJ |
| issn | 2536-7153 |
| language | English |
| publishDate | 2020-03-01 |
| publisher | Hammer Head Production Limited |
| record_format | Article |
| series | Sokoto Journal of Medical Laboratory Science |
| spelling | doaj-art-ab32ab092fc6485bb701d190ed52bcde2025-08-20T02:51:38ZengHammer Head Production LimitedSokoto Journal of Medical Laboratory Science2536-71532020-03-0151146Distribution of TEM, SHV, OXA AND CTX-M Genes among ESBL- producing E. coli Isolates from Patients with Urinary Tract Infections.Olley Mitsan , Maureen U. Okwu , Osakue Eguagie Osareniro , Alao JamesThe emergence of extended-spectrum beta-lactamases (ESBLs) are as a result of transmission among Gram negative bacteria, usually by horizontal gene transfer. The epidemiology of ESBL genes is changing rapidly and shows marked geographic differences in distribution of genotypes. In this study, isolation of extended- spectrum beta- lactamase producing E. coli in patients with bacteriuria in parts of Edo state, Nigeria was carried out using the urine of patients with UTI in selected teaching hospitals namely : University of Benin Teaching Hospital (UBTH), Irrua Specialist Teaching Hospital, Irrua (ISTH) and Igbinedion University Teaching Hospital, Okada (IUTH). A total of 3,378 urine samples were cultured into Blood agar, Cysteine lactose electrolyte deficient (CLED) agar and MacConkey agar and incubated aerobically at 37°C. Isolated E. coli had resistance of 38.5%, 43.9%, 36.4%, 38.5% and 52% to cefuroxime, ceftazidime, cefixime, ceftatriaxone and cefotaxime respectively. Phenotypic detection of ESBL producing E. coli was done using the double disk synergism. ESBL- producing E. coli had prevalence of 1.5%, genomic DNA extraction was carried out on the E. coli that were ESBL positive and hybridization effected using specific primers for the assayed genes. The BlaTEM, BlaCTX, BlaOXA and BlaSHV genes had prevalence of 33.3%, 53.3%, 6.7% and 6.7% respectively. This study has established the presence of these resistant genes among the E. coli isolates from patients with urinary tract infections.https://sokjmls.com.ng/index.php/SJMLS/article/view/146extended spectrum beta- lactamase, e. coli |
| spellingShingle | Olley Mitsan , Maureen U. Okwu , Osakue Eguagie Osareniro , Alao James Distribution of TEM, SHV, OXA AND CTX-M Genes among ESBL- producing E. coli Isolates from Patients with Urinary Tract Infections. Sokoto Journal of Medical Laboratory Science extended spectrum beta- lactamase, e. coli |
| title | Distribution of TEM, SHV, OXA AND CTX-M Genes among ESBL- producing E. coli Isolates from Patients with Urinary Tract Infections. |
| title_full | Distribution of TEM, SHV, OXA AND CTX-M Genes among ESBL- producing E. coli Isolates from Patients with Urinary Tract Infections. |
| title_fullStr | Distribution of TEM, SHV, OXA AND CTX-M Genes among ESBL- producing E. coli Isolates from Patients with Urinary Tract Infections. |
| title_full_unstemmed | Distribution of TEM, SHV, OXA AND CTX-M Genes among ESBL- producing E. coli Isolates from Patients with Urinary Tract Infections. |
| title_short | Distribution of TEM, SHV, OXA AND CTX-M Genes among ESBL- producing E. coli Isolates from Patients with Urinary Tract Infections. |
| title_sort | distribution of tem shv oxa and ctx m genes among esbl producing e coli isolates from patients with urinary tract infections |
| topic | extended spectrum beta- lactamase, e. coli |
| url | https://sokjmls.com.ng/index.php/SJMLS/article/view/146 |
| work_keys_str_mv | AT olleymitsanmaureenuokwuosakueeguagieosareniroalaojames distributionoftemshvoxaandctxmgenesamongesblproducingecoliisolatesfrompatientswithurinarytractinfections |