Massive regime shifts and high activity of heterotrophic bacteria in an ice-covered lake.
In winter 2009/10, a sudden under-ice bloom of heterotrophic bacteria occurred in the seasonally ice-covered, temperate, deep, oligotrophic Lake Stechlin (Germany). Extraordinarily high bacterial abundance and biomass were fueled by the breakdown of a massive bloom of Aphanizomenon flos-aquae after...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0113611&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850191609390432256 |
|---|---|
| author | Mina Bižić-Ionescu Rudolf Amann Hans-Peter Grossart |
| author_facet | Mina Bižić-Ionescu Rudolf Amann Hans-Peter Grossart |
| author_sort | Mina Bižić-Ionescu |
| collection | DOAJ |
| description | In winter 2009/10, a sudden under-ice bloom of heterotrophic bacteria occurred in the seasonally ice-covered, temperate, deep, oligotrophic Lake Stechlin (Germany). Extraordinarily high bacterial abundance and biomass were fueled by the breakdown of a massive bloom of Aphanizomenon flos-aquae after ice formation. A reduction in light resulting from snow coverage exerted a pronounced physiological stress on the cyanobacteria. Consequently, these were rapidly colonized, leading to a sudden proliferation of attached and subsequently of free-living heterotrophic bacteria. Total bacterial protein production reached 201 µg C L(-1) d(-1), ca. five times higher than spring-peak values that year. Fluorescence in situ hybridization and denaturing gradient gel electrophoresis at high temporal resolution showed pronounced changes in bacterial community structure coinciding with changes in the physiology of the cyanobacteria. Pyrosequencing of 16S rRNA genes revealed that during breakdown of the cyanobacterial population, the diversity of attached and free-living bacterial communities were reduced to a few dominant families. Some of these were not detectable during the early stages of the cyanobacterial bloom indicating that only specific, well adapted bacterial communities can colonize senescent cyanobacteria. Our study suggests that in winter, unlike commonly postulated, carbon rather than temperature is the limiting factor for bacterial growth. Frequent phytoplankton blooms in ice-covered systems highlight the need for year-round studies of aquatic ecosystems including the winter season to correctly understand element and energy cycling through aquatic food webs, particularly the microbial loop. On a global scale, such knowledge is required to determine climate change induced alterations in carbon budgets in polar and temperate aquatic systems. |
| format | Article |
| id | doaj-art-ab2f868ccedc4e059d16f72b2680a561 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-ab2f868ccedc4e059d16f72b2680a5612025-08-20T02:14:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11361110.1371/journal.pone.0113611Massive regime shifts and high activity of heterotrophic bacteria in an ice-covered lake.Mina Bižić-IonescuRudolf AmannHans-Peter GrossartIn winter 2009/10, a sudden under-ice bloom of heterotrophic bacteria occurred in the seasonally ice-covered, temperate, deep, oligotrophic Lake Stechlin (Germany). Extraordinarily high bacterial abundance and biomass were fueled by the breakdown of a massive bloom of Aphanizomenon flos-aquae after ice formation. A reduction in light resulting from snow coverage exerted a pronounced physiological stress on the cyanobacteria. Consequently, these were rapidly colonized, leading to a sudden proliferation of attached and subsequently of free-living heterotrophic bacteria. Total bacterial protein production reached 201 µg C L(-1) d(-1), ca. five times higher than spring-peak values that year. Fluorescence in situ hybridization and denaturing gradient gel electrophoresis at high temporal resolution showed pronounced changes in bacterial community structure coinciding with changes in the physiology of the cyanobacteria. Pyrosequencing of 16S rRNA genes revealed that during breakdown of the cyanobacterial population, the diversity of attached and free-living bacterial communities were reduced to a few dominant families. Some of these were not detectable during the early stages of the cyanobacterial bloom indicating that only specific, well adapted bacterial communities can colonize senescent cyanobacteria. Our study suggests that in winter, unlike commonly postulated, carbon rather than temperature is the limiting factor for bacterial growth. Frequent phytoplankton blooms in ice-covered systems highlight the need for year-round studies of aquatic ecosystems including the winter season to correctly understand element and energy cycling through aquatic food webs, particularly the microbial loop. On a global scale, such knowledge is required to determine climate change induced alterations in carbon budgets in polar and temperate aquatic systems.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0113611&type=printable |
| spellingShingle | Mina Bižić-Ionescu Rudolf Amann Hans-Peter Grossart Massive regime shifts and high activity of heterotrophic bacteria in an ice-covered lake. PLoS ONE |
| title | Massive regime shifts and high activity of heterotrophic bacteria in an ice-covered lake. |
| title_full | Massive regime shifts and high activity of heterotrophic bacteria in an ice-covered lake. |
| title_fullStr | Massive regime shifts and high activity of heterotrophic bacteria in an ice-covered lake. |
| title_full_unstemmed | Massive regime shifts and high activity of heterotrophic bacteria in an ice-covered lake. |
| title_short | Massive regime shifts and high activity of heterotrophic bacteria in an ice-covered lake. |
| title_sort | massive regime shifts and high activity of heterotrophic bacteria in an ice covered lake |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0113611&type=printable |
| work_keys_str_mv | AT minabizicionescu massiveregimeshiftsandhighactivityofheterotrophicbacteriainanicecoveredlake AT rudolfamann massiveregimeshiftsandhighactivityofheterotrophicbacteriainanicecoveredlake AT hanspetergrossart massiveregimeshiftsandhighactivityofheterotrophicbacteriainanicecoveredlake |