Phenotypic and Complete Reference Whole Genome Sequence Analyses of Two <i>Paenibacillus</i> spp. Isolates from a Gray Wolf (<i>Canis lupus</i>) Gastrointestinal Tract
Inflammatory bowel disease (IBD) is increasing among mammals around the world, and domestic dogs are no exception. There is no approved cure for canine IBD with limited treatment options. Novel probiotic bacteria discovery from free-ranging animals for the treatment of IBD in domestic pets can likel...
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2025-01-01
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| author | Jessika L. Bryant Jennifer McCabe C. Cristoph Klews MiCayla Johnson Ariel N. Atchley Thomas W. Cousins Maya Barnard-Davidson Kristina M. Smith Mark R. Ackermann Michael Netherland Nur A. Hasan Peter A. Jordan Evan S. Forsythe Patrick N. Ball Bruce S. Seal |
| author_facet | Jessika L. Bryant Jennifer McCabe C. Cristoph Klews MiCayla Johnson Ariel N. Atchley Thomas W. Cousins Maya Barnard-Davidson Kristina M. Smith Mark R. Ackermann Michael Netherland Nur A. Hasan Peter A. Jordan Evan S. Forsythe Patrick N. Ball Bruce S. Seal |
| author_sort | Jessika L. Bryant |
| collection | DOAJ |
| description | Inflammatory bowel disease (IBD) is increasing among mammals around the world, and domestic dogs are no exception. There is no approved cure for canine IBD with limited treatment options. Novel probiotic bacteria discovery from free-ranging animals for the treatment of IBD in domestic pets can likely yield promising probiotic candidates. Consequently, the overall aim was to isolate bacteria from free-ranging animals that could potentially be utilized as novel probiotics. Two bacteria identified as unique <i>Paenibacillus</i> spp. strains by small ribosomal RNA (16S) gene sequencing were isolated from the gastrointestinal tract of a North American Gray Wolf (<i>Canis lupus</i>). The bacteria were typed as Gram-variable, and both were catalase/oxidase positive as well as sensitive to commonly used antibiotics. The bacteria digested complex carbohydrates and lipids by standard assays. The isolated bacteria also inhibited the growth of <i>Staphylococcus aureus</i> and <i>Micrococcus luteus</i>. The whole genome sequence (WGS) length of bacterial isolate ClWae17B was 6,939,193 bp, while ClWae19 was 7,032,512 bp, both similar in size to other <i>Paenibacillus</i> spp. The genomes of both bacteria encoded enzymes involved with the metabolism of complex starches and lipids, such as lyases and pectinases, along with encoding antimicrobials such as lanthipeptides, lasso peptides, and cyclic-lactone-autoinducers. No pernicious virulence genes were identified in the WGS of either bacterial isolate. Phylogenetically, the most closely related bacteria based on 16S gene sequences and WGS were <i>P. taichungensis</i> for ClWae17B and <i>P. amylolyticus</i> for ClWae19. WGS analyses and phenotypic assays supported the hypothesis that the isolates described constitute two novel candidate probiotic bacteria for potential use in dogs. |
| format | Article |
| id | doaj-art-ab29ca0b8ebb4cb08bb0bf1e8c8e557c |
| institution | Kabale University |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Veterinary Sciences |
| spelling | doaj-art-ab29ca0b8ebb4cb08bb0bf1e8c8e557c2025-01-24T13:52:07ZengMDPI AGVeterinary Sciences2306-73812025-01-011215110.3390/vetsci12010051Phenotypic and Complete Reference Whole Genome Sequence Analyses of Two <i>Paenibacillus</i> spp. Isolates from a Gray Wolf (<i>Canis lupus</i>) Gastrointestinal TractJessika L. Bryant0Jennifer McCabe1C. Cristoph Klews2MiCayla Johnson3Ariel N. Atchley4Thomas W. Cousins5Maya Barnard-Davidson6Kristina M. Smith7Mark R. Ackermann8Michael Netherland9Nur A. Hasan10Peter A. Jordan11Evan S. Forsythe12Patrick N. Ball13Bruce S. Seal14Biology Program, Oregon State University-Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University-Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University-Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University-Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University-Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University-Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University-Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University-Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USAOregon Veterinary Diagnostic Laboratory, OSU Carlson College of Veterinary Medicine, 134 Magruder Hall, 700 SW 30th, Corvallis, OR 97331, USAEzBiome Inc., 704 Quince Orchard Rd Suite 250, Gaithersburg, MD 20878, USAEzBiome Inc., 704 Quince Orchard Rd Suite 250, Gaithersburg, MD 20878, USABiology Program, Oregon State University-Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University-Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University-Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USABiology Program, Oregon State University-Cascades, 1500 SW Chandler Avenue, Bend, OR 97702, USAInflammatory bowel disease (IBD) is increasing among mammals around the world, and domestic dogs are no exception. There is no approved cure for canine IBD with limited treatment options. Novel probiotic bacteria discovery from free-ranging animals for the treatment of IBD in domestic pets can likely yield promising probiotic candidates. Consequently, the overall aim was to isolate bacteria from free-ranging animals that could potentially be utilized as novel probiotics. Two bacteria identified as unique <i>Paenibacillus</i> spp. strains by small ribosomal RNA (16S) gene sequencing were isolated from the gastrointestinal tract of a North American Gray Wolf (<i>Canis lupus</i>). The bacteria were typed as Gram-variable, and both were catalase/oxidase positive as well as sensitive to commonly used antibiotics. The bacteria digested complex carbohydrates and lipids by standard assays. The isolated bacteria also inhibited the growth of <i>Staphylococcus aureus</i> and <i>Micrococcus luteus</i>. The whole genome sequence (WGS) length of bacterial isolate ClWae17B was 6,939,193 bp, while ClWae19 was 7,032,512 bp, both similar in size to other <i>Paenibacillus</i> spp. The genomes of both bacteria encoded enzymes involved with the metabolism of complex starches and lipids, such as lyases and pectinases, along with encoding antimicrobials such as lanthipeptides, lasso peptides, and cyclic-lactone-autoinducers. No pernicious virulence genes were identified in the WGS of either bacterial isolate. Phylogenetically, the most closely related bacteria based on 16S gene sequences and WGS were <i>P. taichungensis</i> for ClWae17B and <i>P. amylolyticus</i> for ClWae19. WGS analyses and phenotypic assays supported the hypothesis that the isolates described constitute two novel candidate probiotic bacteria for potential use in dogs.https://www.mdpi.com/2306-7381/12/1/51genomicsprobioticcanineantimicrobial<i>Paenibacillaceae</i> |
| spellingShingle | Jessika L. Bryant Jennifer McCabe C. Cristoph Klews MiCayla Johnson Ariel N. Atchley Thomas W. Cousins Maya Barnard-Davidson Kristina M. Smith Mark R. Ackermann Michael Netherland Nur A. Hasan Peter A. Jordan Evan S. Forsythe Patrick N. Ball Bruce S. Seal Phenotypic and Complete Reference Whole Genome Sequence Analyses of Two <i>Paenibacillus</i> spp. Isolates from a Gray Wolf (<i>Canis lupus</i>) Gastrointestinal Tract Veterinary Sciences genomics probiotic canine antimicrobial <i>Paenibacillaceae</i> |
| title | Phenotypic and Complete Reference Whole Genome Sequence Analyses of Two <i>Paenibacillus</i> spp. Isolates from a Gray Wolf (<i>Canis lupus</i>) Gastrointestinal Tract |
| title_full | Phenotypic and Complete Reference Whole Genome Sequence Analyses of Two <i>Paenibacillus</i> spp. Isolates from a Gray Wolf (<i>Canis lupus</i>) Gastrointestinal Tract |
| title_fullStr | Phenotypic and Complete Reference Whole Genome Sequence Analyses of Two <i>Paenibacillus</i> spp. Isolates from a Gray Wolf (<i>Canis lupus</i>) Gastrointestinal Tract |
| title_full_unstemmed | Phenotypic and Complete Reference Whole Genome Sequence Analyses of Two <i>Paenibacillus</i> spp. Isolates from a Gray Wolf (<i>Canis lupus</i>) Gastrointestinal Tract |
| title_short | Phenotypic and Complete Reference Whole Genome Sequence Analyses of Two <i>Paenibacillus</i> spp. Isolates from a Gray Wolf (<i>Canis lupus</i>) Gastrointestinal Tract |
| title_sort | phenotypic and complete reference whole genome sequence analyses of two i paenibacillus i spp isolates from a gray wolf i canis lupus i gastrointestinal tract |
| topic | genomics probiotic canine antimicrobial <i>Paenibacillaceae</i> |
| url | https://www.mdpi.com/2306-7381/12/1/51 |
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