Enhancing Biomass Production of <i>Chlorella vulgaris</i> in Anaerobically Digested Swine Wastewater Using Carbon Supplementation and Simultaneous Lipid Production
This study investigated anaerobically digested swine wastewater (ADSW) as a nutrient source for <i>Chlorella vulgaris</i> FACHB-8 cultivation under mixotrophic conditions with carbon supplementation. The microalgal strain was grown in ADSW supplemented with six carbon sources, followed b...
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MDPI AG
2025-05-01
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| Online Access: | https://www.mdpi.com/2076-3417/15/9/5103 |
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| author | Chenkai Zhou Mingmin Yuan Cuifeng Huang Qiqi Chen Jiamin Wang Xinting Chen Hua Yang Jun Fang Bo Yang |
| author_facet | Chenkai Zhou Mingmin Yuan Cuifeng Huang Qiqi Chen Jiamin Wang Xinting Chen Hua Yang Jun Fang Bo Yang |
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| description | This study investigated anaerobically digested swine wastewater (ADSW) as a nutrient source for <i>Chlorella vulgaris</i> FACHB-8 cultivation under mixotrophic conditions with carbon supplementation. The microalgal strain was grown in ADSW supplemented with six carbon sources, followed by concentration optimization. Under optimized conditions (20 g/L glucose), FACHB-8 demonstrated a high biomass productivity (271.31 mg/L/day) and a specific growth rate of 0.42 per day. The system achieved an 88.70% total nitrogen removal and an 82.93% total phosphorus removal. The biomass contained 45.59% lipids, 29.72% proteins, and 13.05% carbohydrates, with fatty acid methyl esters showing balanced proportions of saturated (50.77%) and unsaturated fatty acids (49.23%). These findings highlight the potential of glucose-based mixotrophic cultivation for simultaneous wastewater treatment, renewable biomass production, and value-added lipid production. This work proposes a scalable swine wastewater treatment system that synergizes bioremediation and renewable energy production via carbon-enhanced microalgae cultivation, offering a dual-functional strategy for sustainable livestock wastewater reuse. |
| format | Article |
| id | doaj-art-ab20fea539db473ebc1abd9cdbf20b18 |
| institution | OA Journals |
| issn | 2076-3417 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
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| series | Applied Sciences |
| spelling | doaj-art-ab20fea539db473ebc1abd9cdbf20b182025-08-20T01:49:24ZengMDPI AGApplied Sciences2076-34172025-05-01159510310.3390/app15095103Enhancing Biomass Production of <i>Chlorella vulgaris</i> in Anaerobically Digested Swine Wastewater Using Carbon Supplementation and Simultaneous Lipid ProductionChenkai Zhou0Mingmin Yuan1Cuifeng Huang2Qiqi Chen3Jiamin Wang4Xinting Chen5Hua Yang6Jun Fang7Bo Yang8College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, ChinaThis study investigated anaerobically digested swine wastewater (ADSW) as a nutrient source for <i>Chlorella vulgaris</i> FACHB-8 cultivation under mixotrophic conditions with carbon supplementation. The microalgal strain was grown in ADSW supplemented with six carbon sources, followed by concentration optimization. Under optimized conditions (20 g/L glucose), FACHB-8 demonstrated a high biomass productivity (271.31 mg/L/day) and a specific growth rate of 0.42 per day. The system achieved an 88.70% total nitrogen removal and an 82.93% total phosphorus removal. The biomass contained 45.59% lipids, 29.72% proteins, and 13.05% carbohydrates, with fatty acid methyl esters showing balanced proportions of saturated (50.77%) and unsaturated fatty acids (49.23%). These findings highlight the potential of glucose-based mixotrophic cultivation for simultaneous wastewater treatment, renewable biomass production, and value-added lipid production. This work proposes a scalable swine wastewater treatment system that synergizes bioremediation and renewable energy production via carbon-enhanced microalgae cultivation, offering a dual-functional strategy for sustainable livestock wastewater reuse.https://www.mdpi.com/2076-3417/15/9/5103wastewatermicroalgaecarbon supplementationbiomassmixotrophy |
| spellingShingle | Chenkai Zhou Mingmin Yuan Cuifeng Huang Qiqi Chen Jiamin Wang Xinting Chen Hua Yang Jun Fang Bo Yang Enhancing Biomass Production of <i>Chlorella vulgaris</i> in Anaerobically Digested Swine Wastewater Using Carbon Supplementation and Simultaneous Lipid Production Applied Sciences wastewater microalgae carbon supplementation biomass mixotrophy |
| title | Enhancing Biomass Production of <i>Chlorella vulgaris</i> in Anaerobically Digested Swine Wastewater Using Carbon Supplementation and Simultaneous Lipid Production |
| title_full | Enhancing Biomass Production of <i>Chlorella vulgaris</i> in Anaerobically Digested Swine Wastewater Using Carbon Supplementation and Simultaneous Lipid Production |
| title_fullStr | Enhancing Biomass Production of <i>Chlorella vulgaris</i> in Anaerobically Digested Swine Wastewater Using Carbon Supplementation and Simultaneous Lipid Production |
| title_full_unstemmed | Enhancing Biomass Production of <i>Chlorella vulgaris</i> in Anaerobically Digested Swine Wastewater Using Carbon Supplementation and Simultaneous Lipid Production |
| title_short | Enhancing Biomass Production of <i>Chlorella vulgaris</i> in Anaerobically Digested Swine Wastewater Using Carbon Supplementation and Simultaneous Lipid Production |
| title_sort | enhancing biomass production of i chlorella vulgaris i in anaerobically digested swine wastewater using carbon supplementation and simultaneous lipid production |
| topic | wastewater microalgae carbon supplementation biomass mixotrophy |
| url | https://www.mdpi.com/2076-3417/15/9/5103 |
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